Cited by 363Neutrophil, Myeloperoxidase and Oxidative MechanismsAntimicrobial Peptides and ActivitiesProtease and Inhibitor Mechanisms

Degradation of Outer Membrane Protein A in Escherichia coli Killing by Neutrophil Elastase

Belaaouaj, Abderr azzaq, Kim, Kwang Sik, Shapiro, Steven D.·2000·Science

Abstract

In determining the mechanism of neutrophil elastase (NE)-mediated killing of Escherichia coli, we found that NE degraded outer membrane protein A (OmpA), localized on the surface of Gram-negative bacteria. NE killed wild-type, but not OmpA-deficient, E. coli. Also, whereas NE-deficient mice had impaired survival in response to E. coli sepsis, as compared to wild-type mice, the presence or absence of NE had no influence on survival in response to sepsis that had been induced with OmpA-deficient E. coli. These findings define a mechanism of nonoxidative bacterial killing by NE and point to OmpA as a bacterial target in host defense.

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Crossref · 10.1126/science.289.5482.1185

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15 of 15 references verified0 citation errors · 0 not checkable

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Authors
Belaaouaj, A. A., Kim, K. S., & Shapiro, S. D.
Affiliations
  • Washington University in St. Louis (US)
  • Children's Hospital Los Angeles (US)
Corresponding author
Belaaouaj, Abderr azzaq
Venue
Science
Published
2000-08-18
Volume
289
Issue
5482
Pages
1185-1187
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2027297714
Cited by
363
References
15
Citations by year
2026: 7 · 2025: 10 · 2024: 9 · 2023: 10 · 2022: 11 · 2021: 15 · 2020: 15 · 2019: 20 · 2018: 24 · 2017: 12 · 2016: 13 · 2015: 16 · 2014: 15 · 2013: 17 · 2012: 15
FWCI
4.95
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords
neutrophil elastase, OmpA, Escherichia coli, neutrophil killing, Gram-negative bacteria, sepsis, host defense, bacterial surface proteins
MeSH terms
Animals, Bacterial Outer Membrane Proteins, Blood Bactericidal Activity, Cell Membrane, Escherichia coli, Escherichia coli Infections, Humans, Microscopy, Electron, Mutation, Neutrophils, Phagosomes, Proteins, Colony Count, Microbial, Serine Proteinase Inhibitors, Leukocyte Elastase, Mice, Proteinase Inhibitory Proteins, Secretory

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