The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
A Transgenic Mouse Model of the Chronic Hepatitis B Surface Antigen Carrier State
Abstract
In an attempt to establish a model of the healthy carrier state in hepatitis B virus (HBV) infections, transgenic mice expressing HBV genes were produced. Fertilized one-cell eggs were microinjected with subgenomic fragments of HBV DNA containing the coding regions for the HBV surface antigen (HBsAg) and pre-S and X antigens. Either the normal (HBV) or metallothionein promoters were used to obtain expression of the HBV genes. There was no evidence of viral replication or tissue pathology. The integrated HBV DNA sequences were inherited in a normal Mendelian fashion. Three of 16 transgenic mice expressed HBV-encoded gene products to which they were immunologically tolerant. Expression was not tissue specific and may be influenced by the genomic integration site and cellular factors. Both HBsAg and pre-S antigen were detectable within the cytoplasm of hepatocytes and renal tubular epithelial cells. High serum concentrations of HBsAg were detectable and the secreted product appeared authentic as judged by mean density, morphology, mean particle diameter, polypeptide composition, and antigenicity. The absence of tissue pathology in these immunologically tolerant animals supports the hypothesis that cellular injury under these conditions is not a direct consequence of expression of the pre-S or HBs regions of the HBV genome.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
HEPATOCELLULAR CARCINOMA AND HEPATITIS B VIRUS
Beasley, R. P., Lin, C.-C., Hwang, L.-Y., & Chien, C.-S. (1981) · The Lancet · doi:10.1016/s0140-6736(81)90585-7 · Cited by 2,865
Summers, J., & Mason, W. S. (1982) · Cell · doi:10.1016/0092-8674(82)90157-x · Cited by 1,443
Palmiter, R. D., Brinster, R. L., Hammer, R. E., Trumbauer, M. E., Rosenfeld, M. G., Birnberg, N. C., & Evans, R. M. (1982) · Nature · doi:10.1038/300611a0 · Cited by 1,340
Self-citationFactors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs.
Brinster, R. L., Chen, H. Y., Trumbauer, M. E., Yagle, M. K., & Palmiter, R. D. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.13.4438 · Cited by 1,027
Self-citationLarge surface proteins of hepatitis B virus containing the pre-s sequence
Heermann, K. H., Goldmann, U., Schwartz, W., Seyffarth, T., Baumgarten, H., & Gerlich, W. H. (1984) · Journal of Virology · doi:10.1128/jvi.52.2.396-402.1984 · Cited by 823
An octamer of histones in chromatin and free in solution.
Thomas, J. O., & Kornberg, R. D. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.7.2626 · Cited by 723
Metallothionein-Human GH Fusion Genes Stimulate Growth of Mice
Palmiter, R. D., Norstedt, G., Gelinas, R. E., Hammer, R. E., & Brinster, R. L. (1983) · Science · doi:10.1126/science.6356363 · Cited by 676
Self-citationLerner, R. A., Green, N., Alexander, H., Liu, F. T., Sutcliffe, J. G., & Shinnick, T. M. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.6.3403 · Cited by 409
Holmes, D. S., & Bonner, J. (1973) · Biochemistry · doi:10.1021/bi00736a023 · Cited by 381
Palmiter, R. D., Chen, H. Y., & Brinster, R. L. (1982) · Cell · doi:10.1016/0092-8674(82)90186-6 · Cited by 369
Self-citation
Cited by
HBx gene of hepatitis B virus induces liver cancer in transgenic mice
Kim, C.-M., Koike, K., Saito, I., Miyamura, T., & Jay, G. (1991) · Nature · doi:10.1038/351317a0 · Cited by 1,158
GERM-LINE TRANSFORMATION OF MICE
Palmiter, R. D., & Brinster, R. L. (1986) · Annual Review of Genetics · doi:10.1146/annurev.ge.20.120186.002341 · Cited by 1,157
Self-citationModels of liver fibrosis: exploring the dynamic nature of inflammation and repair in a solid organ
Iredale, J. P. (2007) · Journal of Clinical Investigation · doi:10.1172/jci30542 · Cited by 866
Molecular pathogenesis of hepatocellular carcinoma in hepatitis B virus transgenic mice
Chisari, F. V., Klopchin, K., Moriyama, T., Pasquinelli, C., Dunsford, H. A., Sell, S., Pinkert, C. A., Brinster, R. L., & Palmiter, R. D. (1989) · Cell · doi:10.1016/0092-8674(89)90770-8 · Cited by 697
Self-citationJaenisch, R. (1988) · Science · doi:10.1126/science.3287623 · Cited by 485
Metadata
- Authors
- Chisari, F. V., Pinkert, C. A., Milich, D. R., Filippi, P., McLachlan, A., Palmiter, R. D., & Brinster, R. L.
- Venue
- Science
- Published
- 1985-12-06
- Volume
- 230
- Issue
- 4730
- Pages
- 1157-1160
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2007706733
- Cited by
- 290
- References
- 16
- FWCI
- 12.31
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A Transgenic Mouse Model of the Chronic Hepatitis B Surface Antigen Carrier State. https://app.cochat.ai/papers/10.1126/science.3865369
- The paper
- Chisari, F. V., Pinkert, C. A., Milich, D. R., Filippi, P., McLachlan, A., Palmiter, R. D., & Brinster, R. L. (1985). A Transgenic Mouse Model of the Chronic Hepatitis B Surface Antigen Carrier State. Science. https://doi.org/10.1126/science.3865369
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
