Cited by 1,023Neurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene RegulationPluripotent Stem Cells Research

Conversion of Xenopus Ectoderm into Neurons by NeuroD, a Basic Helix-Loop-Helix Protein

Lee, Jacqueline E., Hollenberg, Stanley M., Snider, Lauren, Turner, David L., Lipnick, Naomi, Weintraub, Harold·1995·Science

Abstract

Basic helix-loop-helix (bHLH) proteins are instrumental in determining cell type during development. A bHLH protein, termed NeuroD, for neurogenic differentiation, has now been identified as a differentiation factor for neurogenesis because (i) it is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons and (ii) ectopic expression of neuroD in Xenopus embryos causes premature differentiation of neuronal precursors. Furthermore, neuroD can convert presumptive epidermal cells into neurons and also act as a neuronal determination gene. However, unlike another previously identified proneural gene (XASH-3), neuroD seems competent to bypass the normal inhibitory influences that usually prevent neurogenesis in ventral and lateral ectoderm and is capable of converting most of the embryonic ectoderm into neurons. The data suggest that neuroD may participate in the terminal differentiation step during vertebrate neuronal development.

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Crossref · 10.1126/science.7754368

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Authors
Lee, J. E., Hollenberg, S. M., Snider, L., Turner, D. L., Lipnick, N., & Weintraub, H.
Affiliations
  • Fred Hutch Cancer Center (US)
  • Oregon Health & Science University (US)
  • Howard Hughes Medical Institute (US)
Venue
Science
Published
1995-05-12
Volume
268
Issue
5212
Pages
836-844
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1972676659
Cited by
1,023
References
101
Citations by year
2026: 6 · 2025: 10 · 2024: 9 · 2023: 12 · 2022: 11 · 2021: 16 · 2020: 16 · 2019: 10 · 2018: 10 · 2017: 13 · 2016: 12 · 2015: 22 · 2014: 23 · 2013: 32 · 2012: 26
FWCI
21.69
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
neurogenic differentiation, basic helix-loop-helix proteins, neuronal differentiation, neurogenesis, Xenopus, epidermal cells, terminal differentiation, proneural genes, ectopic expression
MeSH terms
Amino Acid Sequence, Animals, Cell Differentiation, Cloning, Molecular, Ectoderm, Molecular Sequence Data, Nerve Tissue Proteins, Nervous System, Neural Crest, Neurons, Xenopus, Cell Adhesion Molecules, Neuronal, Sequence Alignment, Helix-Loop-Helix Motifs, Gene Expression Regulation, Developmental, Mice, Basic Helix-Loop-Helix Transcription Factors, Basic Helix-Loop-Helix Proteins

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