The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Stoichiometry controls activity of phase-separated clusters of actin signaling proteins
Open accessAbstract
Organized for action: It is becoming increasingly clear that biomolecular condensates, which are concentrations of macromolecules not surrounded by a membrane, are a key organizational structure in eukaryotic cells (see the Perspective by Martin and Mittag). Now, two papers show how such condensates function in actin assembly or in a Ras signaling pathway. In both cases, the condensates form at the plasma membrane and increase the activity of signaling proteins by increasing their membrane dwell times. Case et al. show that the dwell time is dependent on cluster stoichiometry, so that stoichiometry of regulatory proteins can control actin assembly. Huang et al. demonstrate that the longer dwell time allows kinetic proofreading in receptor-mediated activation of Ras. Science , this issue p. 1093 , p. 1098 ; see also p. 1036
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Cites
Biomolecular condensates: organizers of cellular biochemistry
Banani, S. F., Lee, H. O., Hyman, A. A., & Rosen, M. K. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.7 · Cited by 6,100
Self-citationLiquid phase condensation in cell physiology and disease
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Phase transitions in the assembly of multivalent signalling proteins
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Self-citationPhase separation of signaling molecules promotes T cell receptor signal transduction
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Self-citationPhase transitions of multivalent proteins can promote clustering of membrane receptors
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Self-citationA molecular assembly phase transition and kinetic proofreading modulate Ras activation by SOS
Huang, W. Y. C., Alvarez, S., Kondo, Y., Lee, Y. K., Chung, J. K., Lam, H. Y. M., Biswas, K. H., Kuriyan, J., & Groves, J. T. (2019) · Science · doi:10.1126/science.aau5721 · Cited by 347
Arp2/3 complex is bound and activated by two WASP proteins
Padrick, S. B., Doolittle, L. K., Brautigam, C. A., King, D. S., & Rosen, M. K. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1100236108 · Cited by 210
Self-citationHierarchical Regulation of WASP/WAVE Proteins
Padrick, S. B., Cheng, H.-C., Ismail, A. M., Panchal, S. C., Doolittle, L. K., Kim, S., Skehan, B. M., Umetani, J., Brautigam, C. A., Leong, J. M., et al. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.10.012 · Cited by 203
Self-citationConserved interdomain linker promotes phase separation of the multivalent adaptor protein Nck
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Self-citation[19] Methods to measure actin polymerization
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Cited by
Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing
Alberti, S., & Hyman, A. A. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00326-6 · Cited by 1,160
A framework for understanding the functions of biomolecular condensates across scales
Lyon, A. S., Peeples, W. B., & Rosen, M. K. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00303-z · Cited by 895
Self-citationPhase separation and neurodegenerative diseases: a disturbance in the force
Zbinden, A., Pérez-Berlanga, M., De Rossi, P., & Polymenidou, M. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.09.014 · Cited by 500
The RHO Family GTPases: Mechanisms of Regulation and Signaling
Mosaddeghzadeh, N., & Ahmadian, M. R. (2021) · Cells · doi:10.3390/cells10071831 · Cited by 361
Regulation of Transmembrane Signaling by Phase Separation
Case, L. B., Ditlev, J. A., & Rosen, M. K. (2019) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-052118-115534 · Cited by 303
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Metadata
- Authors
- Case, L. B., Zhang, X., Ditlev, J. A., & Rosen, M. K.
- Venue
- Science
- Published
- 2019-03-08
- Volume
- 363
- Issue
- 6431
- Pages
- 1093-1097
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2922246560
- Cited by
- 499
- References
- 26
- FWCI
- 18.12
- Type
- journal article
- Language
- en
- Open access
- green
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