An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

Schoof, Michael; Faust, Bryan; Saunders, Reuben A.; Sangwan, Smriti; Rezelj, Veronica; Hoppe, Nick; Boone, Morgane; Billesbølle, Christian B.; Puchades, Cristina; Azumaya, Caleigh M.; Kratochvil, Huong T.; Zimanyi, Marcell; Deshpande, Ishan; Liang, Jiahao; Dickinson, Sasha; Nguyen, Henry C.; Chio, Cynthia M.; Merz, Gregory E.; Thompson, Michael C.; Diwanji, Devan; Schaefer, Kaitlin; Anand, Aditya A.; Dobzinski, Niv; Zha, Beth Shoshana; Simoneau, Camille R.; Leon, Kristoffer; White, Kris M.; Chio, Un Seng; Gupta, Meghna; Jin, Mingliang; Li, Fei; Liu, Yanxin; Zhang, Kaihua; Bulkley, David; Sun, Ming; Smith, Amber M.; Rizo, Alexandrea N.; Moss, Frank; Brilot, Axel F.; Pourmal, Sergei; Trenker, Raphael; Pospiech, Thomas; Gupta, Sayan; Barsi-Rhyne, Benjamin; Belyy, Vladislav; Barile-Hill, Andrew W.; Nock, Silke; Liu, Yuwei; Krogan, Nevan J.; Ralston, Corie Y.

doi:10.1126/science.abe3255

Cited by 449

The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.

An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

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Schoof, Michael, Faust, Bryan, Saunders, Reuben A., Sangwan, Smriti, Rezelj, Veronica, Hoppe, Nick, Boone, Morgane, Billesbølle, Christian B., Puchades, Cristina, Azumaya, Caleigh M., Kratochvil, Huong T., Zimanyi, Marcell, Deshpande, Ishan, Liang, Jiahao, Dickinson, Sasha, Nguyen, Henry C., Chio, Cynthia M., Merz, Gregory E., Thompson, Michael C., Diwanji, Devan, Schaefer, Kaitlin, Anand, Aditya A., Dobzinski, Niv, Zha, Beth Shoshana, Simoneau, Camille R., Leon, Kristoffer, White, Kris M., Chio, Un Seng, Gupta, Meghna, Jin, Mingliang, Li, Fei, Liu, Yanxin, Zhang, Kaihua, Bulkley, David, Sun, Ming, Smith, Amber M., Rizo, Alexandrea N., Moss, Frank, Brilot, Axel F., Pourmal, Sergei, Trenker, Raphael, Pospiech, Thomas, Gupta, Sayan, Barsi-Rhyne, Benjamin, Belyy, Vladislav, Barile-Hill, Andrew W., Nock, Silke, Liu, Yuwei, Krogan, Nevan J., Ralston, Corie Y., Swaney, Danielle L., García-Sastre, Adolfo, Ott, Melanie, Vignuzzi, Marco, QCRG Structural Biology Consortium, Walter, Peter, Manglik, Aashish, Azumaya, Caleigh M., Puchades, Cristina, Sun, Ming, Braxton, Julian R., Brilot, Axel F., Gupta, Meghna, Li, Fei, Lopez, Kyle E., Melo, Arthur, Merz, Gregory E., Moss, Frank, Paulino, Joana, Pospiech, Thomas H., Pourmal, Sergei, Rizo, Alexandrea N., Smith, Amber M., Thomas, Paul V., Wang, Feng, Yu, Zanlin, Dickinson, Miles Sasha, Nguyen, Henry C., Asarnow, Daniel, Campbell, Melody G., Chio, Cynthia M., Chio, Un Seng, Diwanji, Devan, Faust, Bryan, Gupta, Meghna, Hoppe, Nick, Jin, Mingliang, Li, Junrui, Liu, Yanxin, Merz, Gregory E., Sangwan, Smriti, Tsui, Tsz Kin Martin, Trenker, Raphael, Trinidad, Donovan, Tse, Eric, Zhang, Kaihua, Zhou, Fengbo, Herrera, Nadia, Kratochvil, Huong T., Schulze-Gahmen, Ursula, Thompson, Michael C., Young, Iris D., Biel, Justin, Deshpande, Ishan, Liu, Xi, Billesbølle, Christian Bache, Nowotny, Carlos, Smith, Amber M., Zhao, Jianhua, Bowen, Alisa, Hoppe, Nick, Li, Yen-Li, Nguyen, Phuong, Safari, Mali, Schaefer, Kaitlin, Whitis, Natalie, Moritz, Michelle, Owens, Tristan W., Diallo, Amy, Kim, Kate, Peters, Jessica K., Titus, Erron W., Chen, Jenny, Doan, Loan, Flores, Sebastian, Lam, Victor L., Li, Yang, Lo, Megan, Thwin, Aye C., Wankowicz, Stephanie, Zhang, Yang, Bulkley, David, Joves, Arceli, Joves, Almarie, McKay, Liam, Tabios, Mariano, Rosenberg, Oren S., Verba, Kliment A., Agard, David A., Cheng, Yifan, Fraser, James S., Frost, Adam, Jura, Natalia, Kortemme, Tanja, Krogan, Nevan J., Manglik, Aashish, Southworth, Daniel R., Stroud, Robert M.·2020·Science

DOI 10.1126/science.abe3255

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Abstract

Nanobodies that neutralize: Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bacteria or yeast, and their stability may enable aerosol delivery. Two papers now report nanobodies that bind tightly to spike and efficiently neutralize SARS-CoV-2 in cells. Schoof et al. screened a yeast surface display of synthetic nanobodies and Xiang et al. screened anti-spike nanobodies produced by a llama. Both groups identified highly potent nanobodies that lock the spike protein in an inactive conformation. Multivalent constructs of selected nanobodies achieved even more potent neutralization. Science , this issue p. 1473 , p. 1479

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Authors: Schoof, M., Faust, B., Saunders, R. A., Sangwan, S., Rezelj, V., Hoppe, N., Boone, M., Billesbølle, C. B., Puchades, C., Azumaya, C. M., Kratochvil, H. T., Zimanyi, M., Deshpande, I., Liang, J., Dickinson, S., Nguyen, H. C., Chio, C. M., Merz, G. E., Thompson, M. C., Diwanji, D., Schaefer, K., Anand, A. A., Dobzinski, N., Zha, B. S., Simoneau, C. R., Leon, K., White, K. M., Chio, U. S., Gupta, M., Jin, M., Li, F., Liu, Y., Zhang, K., Bulkley, D., Sun, M., Smith, A. M., Rizo, A. N., Moss, F., Brilot, A. F., Pourmal, S., Trenker, R., Pospiech, T., Gupta, S., Barsi-Rhyne, B., Belyy, V., Barile-Hill, A. W., Nock, S., Liu, Y., Krogan, N. J., Ralston, C. Y., Swaney, D. L., García-Sastre, A., Ott, M., Vignuzzi, M., QCRG Structural Biology Consortium, Walter, P., Manglik, A., Azumaya, C. M., Puchades, C., Sun, M., Braxton, J. R., Brilot, A. F., Gupta, M., Li, F., Lopez, K. E., Melo, A., Merz, G. E., Moss, F., Paulino, J., Pospiech, T. H., Pourmal, S., Rizo, A. N., Smith, A. M., Thomas, P. V., Wang, F., Yu, Z., Dickinson, M. S., Nguyen, H. C., Asarnow, D., Campbell, M. G., Chio, C. M., Chio, U. S., Diwanji, D., Faust, B., Gupta, M., Hoppe, N., Jin, M., Li, J., Liu, Y., Merz, G. E., Sangwan, S., Tsui, T. K. M., Trenker, R., Trinidad, D., Tse, E., Zhang, K., Zhou, F., Herrera, N., Kratochvil, H. T., Schulze-Gahmen, U., Thompson, M. C., Young, I. D., Biel, J., Deshpande, I., Liu, X., Billesbølle, C. B., Nowotny, C., Smith, A. M., Zhao, J., Bowen, A., Hoppe, N., Li, Y.-L., Nguyen, P., Safari, M., Schaefer, K., Whitis, N., Moritz, M., Owens, T. W., Diallo, A., Kim, K., Peters, J. K., Titus, E. W., Chen, J., Doan, L., Flores, S., Lam, V. L., Li, Y., Lo, M., Thwin, A. C., Wankowicz, S., Zhang, Y., Bulkley, D., Joves, A., Joves, A., McKay, L., Tabios, M., Rosenberg, O. S., Verba, K. A., Agard, D. A., Cheng, Y., Fraser, J. S., Frost, A., Jura, N., Kortemme, T., Krogan, N. J., Manglik, A., Southworth, D. R., & Stroud, R. M.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2020-12-18
Volume: 370
Issue: 6523
Pages: 1473-1479
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.abe3255
OpenAlex: W3096751000
Cited by: 449
References: 22
FWCI: 10.89
Type: journal article
Language: en
Open access: hybrid

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This page: CoChat. (2026). Citation check: An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike. https://app.cochat.ai/papers/10.1126/science.abe3255
The paper: Schoof, M., Faust, B., Saunders, R. A., Sangwan, S., Rezelj, V., Hoppe, N., Boone, M., Billesbølle, C. B., Puchades, C., Azumaya, C. M., Kratochvil, H. T., Zimanyi, M., Deshpande, I., Liang, J., Dickinson, S., Nguyen, H. C., Chio, C. M., Merz, G. E., Thompson, M. C., . . . Stroud, R. M. (2020). An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike. Science. https://doi.org/10.1126/science.abe3255

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An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

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