The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Multiplex PCR Strategy for Rapid Identification of Structural Types and Variants of the mec Element in Methicillin-Resistant Staphylococcus aureus
Open accessAbstract
Full characterization of methicillin-resistant Staphylococcus aureus (MRSA) requires definition of not only the bacterial genetic background but also the structure of the complex and heterologous mec element these bacteria carry, which is associated with drug resistance determinant mecA . We report the development, validation, and application of a multiplex PCR strategy that allows quick presumptive characterization of the mec element types based on the structural features that were shown to be typical of mec elements carried by several MRSA clones. The strategy was validated by using a representative collection of pandemic MRSA clones in which the full structure of the associated mec elements was previously determined by hybridization and PCR screenings and also by DNA sequencing. The method was tested together with multilocus sequence typing and other typing methods for the characterization of 18 isolates representative of the MRSA clones recovered during a hospital outbreak in Barcelona, Spain. The multiplex PCR was shown to be rapid, robust, and capable in a single assay of identifying five structural types of the mec element among these strains, three major and two minor variants, each one of which has been already been seen among MRSA characterized earlier. This technique should be a useful addition to the armamentarium of molecular typing tools for the characterization of MRSA clonal types and for the rapid tentative identification of structural variants of the mec element.
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Oliveira, D. C., Tomasz, A., & de Lencastre, H. (2002) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(02)00227-x · Cited by 494
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Cited by
Miller, L. G., Perdreau-Remington, F., Rieg, G., Mehdi, S., Perlroth, J., Bayer, A. S., Tang, A. W., Phung, T. O., & Spellberg, B. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa042683 · Cited by 1,053
Community-acquired meticillin-resistant Staphylococcus aureus: an emerging threat
Zetola, N., Francis, J. S., Nuermberger, E. L., & Bishai, W. R. (2005) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(05)70112-2 · Cited by 791
The molecular evolution of methicillin-resistant Staphylococcus aureus
Deurenberg, R. H., Vink, C., Kalenic, S., Friedrich, A. W., Bruggeman, C. A., & Stobberingh, E. E. (2007) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2006.01573.x · Cited by 597
Frazee, B. W., Lynn, J., Charlebois, E. D., Lambert, L., Lowery, D., & Perdreau-Remington, F. (2005) · Annals of Emergency Medicine · doi:10.1016/j.annemergmed.2004.10.011 · Cited by 494
Saiman, L., Keefe, M. O., Graham, P. L., Wu, F., Salim, B. S., Kreiswirth, B., LaSala, A., Schlievert, P. M., & Latta, P. D. (2003) · Clinical Infectious Diseases · doi:10.1086/379022 · Cited by 372
Liu, C., Graber, C. J., Karr, M., Diep, B. A., Basuino, L., Schwartz, B. S., Enright, M. C., O’Hanlon, S. J., Thomas, J. C., Perdreau‐Remington, F., et al. (2008) · Clinical Infectious Diseases · doi:10.1086/587893 · Cited by 205
Bae, I., Federspiel, J. J., Miró, J. M., Woods, C. W., Park, L., Rybak, M. J., Rude, T. H., Bradley, S., Bukovski, S., de la Maria, C. G., et al. (2009) · The Journal of Infectious Diseases · doi:10.1086/606027 · Cited by 122
Comparative Genomics of Staphylococcus aureus Musculoskeletal Isolates
Cassat, J. E., Dunman, P. M., McAleese, F., Murphy, E., Projan, S. J., & Smeltzer, M. S. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.2.576-592.2005 · Cited by 112
Simor, A. E., Gilbert, N. L., Gravel, D., Mulvey, M. R., Bryce, E., Loeb, M., Matlow, A., McGeer, A., Louie, L., Campbell, J., et al. (2010) · Infection Control & Hospital Epidemiology · doi:10.1086/651313 · Cited by 87
Donnio, P.-Y., Oliveira, D. C., Faria, N. A., Wilhelm, N., Le Coustumier, A., & de Lencastre, H. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.8.4191-4193.2005 · Cited by 82
Self-citationNovel Mobile Variants of Staphylococcal Cassette Chromosome mec in Staphylococcus aureus
Jansen, W. T. M., Beitsma, M. M., Koeman, C. J., van Wamel, W. J. B., Verhoef, J., & Fluit, A. C. (2006) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01539-05 · Cited by 57
May, A. K., Brady, J. S., Romano-Keeler, J., Drake, W. P., Norris, P. R., Jenkins, J. M., Isaacs, R. J., & Boczko, E. M. (2015) · Chest · doi:10.1378/chest.14-1687 · Cited by 40
Corkill, J. E. (2004) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkh284 · Cited by 38
Chen, C.-H., Kuo, K.-C., Hwang, K.-P., Lin, T.-Y., & Huang, Y.-C. (2019) · Journal of Microbiology, Immunology and Infection · doi:10.1016/j.jmii.2018.09.003 · Cited by 18
Metadata
- Authors
- Oliveira, D. C., & Lencastre, H. D.
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2002-07
- Volume
- 46
- Issue
- 7
- Pages
- 2155-2161
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- OpenAlex
- W2149425658
- Cited by
- 1,343
- References
- 1
- FWCI
- 23.95
- Type
- journal article
- Language
- en
- Open access
- green
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