The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG , nirK , and nosZ Genes in Soils
Abstract
Nitrous oxide (N 2 O) is an important greenhouse gas in the troposphere controlling ozone concentration in the stratosphere through nitric oxide production. In order to quantify bacteria capable of N 2 O reduction, we developed a SYBR green quantitative real-time PCR assay targeting the nosZ gene encoding the catalytic subunit of the nitrous oxide reductase. Two independent sets of nosZ primers flanking the nosZ fragment previously used in diversity studies were designed and tested (K. Kloos, A. Mergel, C. Rösch, and H. Bothe, Aust. J. Plant Physiol. 28:991-998, 2001). The utility of these real-time PCR assays was demonstrated by quantifying the nosZ gene present in six different soils. Detection limits were between 10 1 and 10 2 target molecules per reaction for all assays. Sequence analysis of 128 cloned quantitative PCR products confirmed the specificity of the designed primers. The abundance of nosZ genes ranged from 10 5 to 10 7 target copies g −1 of dry soil, whereas genes for 16S rRNA were found at 10 8 to 10 9 target copies g −1 of dry soil. The abundance of narG and nirK genes was within the upper and lower limits of the 16S rRNA and nosZ gene copy numbers. The two sets of nosZ primers gave similar gene copy numbers for all tested soils. The maximum abundance of nosZ and nirK relative to 16S rRNA was 5 to 6%, confirming the low proportion of denitrifiers to total bacteria in soils.
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Cites
Cell biology and molecular basis of denitrification.
Zumft, W. G. (1997) · Microbiology and molecular biology reviews : MMBR · doi:10.1128/.61.4.533-616.1997 · Cited by 3,471
Biology of anaerobic microorganisms
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The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58
Wood, D. W., Setubal, J. C., Kaul, R., Monks, D. E., Kitajima, J. P., Okura, V. K., Zhou, Y., Chen, L., Wood, G. E., Almeida, N. F., et al. (2001) · Science · doi:10.1126/science.1066804 · Cited by 789
DNA Extraction from Soils: Old Bias for New Microbial Diversity Analysis Methods
Martin-Laurent, F., Philippot, L., Hallet, S., Chaussod, R., Germon, J. C., Soulas, G., & Catroux, G. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.5.2354-2359.2001 · Cited by 727
Self-citationQuantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR
Henry, S., Baudoin, E., López-Gutiérrez, J. C., Martin-Laurent, F., Brauman, A., & Philippot, L. (2004) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2004.07.002 · Cited by 698
Self-citationBiodiversity of Denitrifying and Dinitrogen-Fixing Bacteria in an Acid Forest Soil
Rösch, C., Mergel, A., & Bothe, H. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.8.3818-3829.2002 · Cited by 585
Denitrifying genes in bacterial and Archaeal genomes
Philippot, L. (2002) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(02)00420-7 · Cited by 508
Self-citationQuantitative Detection of Methanotrophs in Soil by Novel pmoA -Targeted Real-Time PCR Assays
Kolb, S., Knief, C., Stubner, S., & Conrad, R. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.5.2423-2429.2003 · Cited by 482
Quantification of a novel group of nitrate-reducing bacteria in the environment by real-time PCR
López-Gutiérrez, J. C., Henry, S., Hallet, S., Martin-Laurent, F., Catroux, G., & Philippot, L. (2004) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2004.02.009 · Cited by 424
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Cited by
Smith, C. J., & Osborn, A. M. (2009) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2008.00629.x · Cited by 937
Jones, C. M., Graf, D. R. H., Bru, D., Philippot, L., & Hallin, S. (2013) · The ISME Journal · doi:10.1038/ismej.2012.125 · Cited by 707
Self-citationKandeler, E., Deiglmayr, K., Tscherko, D., Bru, D., & Philippot, L. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.00439-06 · Cited by 630
Self-citationSaggar, S., Jha, N., Deslippe, J., Bolan, N. S., Luo, J., Giltrap, D. L., Kim, D.-G., Zaman, M., & Tillman, R. W. (2013) · Science of The Total Environment · doi:10.1016/j.scitotenv.2012.11.050 · Cited by 543
Ecology of Denitrifying Prokaryotes in Agricultural Soil
Philippot, L., Hallin, S., & Schloter, M. (2007) · Advances in Agronomy · doi:10.1016/s0065-2113(07)96003-4 · Cited by 469
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Metadata
- Authors
- Henry, S., Bru, D., Stres, B., Hallet, S., & Philippot, L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2006-08
- Volume
- 72
- Issue
- 8
- Pages
- 5181-5189
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1970341101
- Cited by
- 984
- References
- 28
- FWCI
- 21.40
- Type
- journal article
- Language
- en
- Open access
- closed
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