The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Microencapsulation of Bacteriophage Felix O1 into Chitosan-Alginate Microspheres for Oral Delivery
Open accessAbstract
This paper reports the development of microencapsulated bacteriophage Felix O1 for oral delivery using a chitosan-alginate-CaCl 2 system. In vitro studies were used to determine the effects of simulated gastric fluid (SGF) and bile salts on the viability of free and encapsulated phage. Free phage Felix O1 was found to be extremely sensitive to acidic environments and was not detectable after a 5-min exposure to pHs below 3.7. In contrast, the number of microencapsulated phage decreased by 0.67 log units only, even at pH 2.4, for the same period of incubation. The viable count of microencapsulated phage decreased only 2.58 log units during a 1-h exposure to SGF with pepsin at pH 2.4. After 3 h of incubation in 1 and 2% bile solutions, the free phage count decreased by 1.29 and 1.67 log units, respectively, while the viability of encapsulated phage was fully maintained. Encapsulated phage was completely released from the microspheres upon exposure to simulated intestinal fluid (pH 6.8) within 6 h. The encapsulated phage in wet microspheres retained full viability when stored at 4°C for the duration of the testing period (6 weeks). With the use of trehalose as a stabilizing agent, the microencapsulated phage in dried form had a 12.6% survival rate after storage for 6 weeks. The current encapsulation technique enables a large proportion of bacteriophage Felix O1 to remain bioactive in a simulated gastrointestinal tract environment, which indicates that these microspheres may facilitate delivery of therapeutic phage to the gut.
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Cited by
Formulation, stabilisation and encapsulation of bacteriophage for phage therapy
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Ménage à trois in the human gut: interactions between host, bacteria and phages
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The factors affecting effectiveness of treatment in phages therapy
Ly-Chatain, M. H. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00051 · Cited by 331
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Metadata
- Authors
- Ma, Y., Pacan, J. C., Wang, Q., Xu, Y., Huang, X., Korenevsky, A., & Sabour, P. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-08
- Volume
- 74
- Issue
- 15
- Pages
- 4799-4805
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2132954220
- Cited by
- 306
- References
- 43
- FWCI
- 3.77
- Type
- journal article
- Language
- en
- Open access
- green
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- Ma, Y., Pacan, J. C., Wang, Q., Xu, Y., Huang, X., Korenevsky, A., & Sabour, P. M. (2008). Microencapsulation of Bacteriophage Felix O1 into Chitosan-Alginate Microspheres for Oral Delivery. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.00246-08
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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