The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
2,3-Butanediol Production by Acetogenic Bacteria, an Alternative Route to Chemical Synthesis, Using Industrial Waste Gas
Open accessAbstract
2,3-Butanediol (23BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, the best microbial 23BD production rates have been observed using pathogenic bacteria in fermentation systems that depend on sugars as the carbon and energy sources for product synthesis. Here we present evidence of 23BD production by three nonpathogenic acetogenic Clostridium species—Clostridium autoethanogenum,C. ljungdahlii, and C. ragsdalei—using carbon monoxide-containing industrial waste gases or syngas as the sole source of carbon and energy. Through an analysis of the C. ljungdahlii genome, the complete pathway from carbon monoxide to 23BD has been proposed. Homologues of the genes involved in this pathway were also confirmed for the other two species investigated. A gene expression study demonstrates a correlation between mRNA accumulation from 23BD biosynthetic genes and the onset of 23BD production, while a broader expression study of Wood-Ljungdahl pathway genes provides a transcription-level view of one of the oldest existing biochemical pathways.
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Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485
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Self-citationMaking quantitative sense of electromicrobial production
Claassens, N. J., Cotton, C. A. R., Kopljar, D., & Bar-Even, A. (2019) · Nature Catalysis · doi:10.1038/s41929-019-0272-0 · Cited by 303
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Metadata
- Authors
- Köpke, M., Mihalcea, C., Liew, F., Tizard, J. H., Ali, M. S., Conolly, J. J., Al-Sinawi, B., & Simpson, S. D.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-08
- Volume
- 77
- Issue
- 15
- Pages
- 5467-5475
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2112168274
- Cited by
- 417
- References
- 51
- FWCI
- 7.76
- Type
- journal article
- Language
- en
- Open access
- green
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- Köpke, M., Mihalcea, C., Liew, F., Tizard, J. H., Ali, M. S., Conolly, J. J., Al-Sinawi, B., & Simpson, S. D. (2011). 2,3-Butanediol Production by Acetogenic Bacteria, an Alternative Route to Chemical Synthesis, Using Industrial Waste Gas. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.00355-11
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