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Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine
Open accessAbstract
The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.
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“Elevated levels of these metabolites suggest reduced dietary nutrient metabolizing bacteria in the oASD microbiota (Matthies et al., 2008, 2009).”
From Human Gut Microbiota from Autism Spectrum Disorder Promote Behavioral Symptoms in Mice (2019) · cites this paper as Matthies et al., 2008
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Metadata
- Authors
- Matthies, A., Clavel, T., Gütschow, M., Engst, W., Haller, D., Blaut, M., & Braune, A.
- Affiliations
- German Institute of Human Nutrition (DE)
- Technical University of Munich (DE)
- Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany
- Department of Gastrointestinal Microbiology
- Corresponding author
- Braune, Annett
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-08
- Volume
- 74
- Issue
- 15
- Pages
- 4847-4852
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2519357
- OpenAlex
- W2067334985
- Cited by
- 141
- References
- 57
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 16 · 2023: 9 · 2022: 15 · 2021: 9 · 2020: 10 · 2019: 11 · 2018: 5 · 2017: 5 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 8 · 2012: 6
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Phytoestrogen effects and research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- daidzein, genistein, isoflavone metabolism, equol, dihydrodaidzein, gut microbiota, Coriobacteriaceae, anaerobic bacteria, enzyme induction, soy isoflavones, mouse intestine, metabolic activation
- MeSH terms
- Animals, Bacteria, Anaerobic, Biotransformation, Chromatography, High Pressure Liquid, Female, Gram-Positive Bacteria, Ileum, Isoflavones, Mice, Inbred C57BL, Molecular Sequence Data, Genistein, Spectrometry, Mass, Electrospray Ionization, Mice
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