The DOI matches its Crossref record; 56 of the 57 references it cites were found in a registry or an index.
Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine
Open accessAbstract
The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Elevated levels of these metabolites suggest reduced dietary nutrient metabolizing bacteria in the oASD microbiota (Matthies et al., 2008, 2009).”
From Human Gut Microbiota from Autism Spectrum Disorder Promote Behavioral Symptoms in Mice (2019) · cites this paper as Matthies et al., 2008
Citations
Cites
Dietary polyphenols and the prevention of diseases
Scalbert, A., Manach, C., Morand, C., Rémésy, C., & Jiménez, L. (2005) · Critical Reviews in Food Science and Nutrition · doi:10.1080/1040869059096 · Cited by 2,999
Impaired On/Off Regulation of TNF Biosynthesis in Mice Lacking TNF AU-Rich Elements
Kontoyiannis, D. L., Pasparakis, M., Pizarro, T. T., Cominelli, F., & Kollias, G. A. (1999) · Immunity · doi:10.1016/s1074-7613(00)80038-2 · Cited by 1,329
Dietary agents in cancer prevention: flavonoids and isoflavonoids
Birt, D. F., Hendrich, S., & Wang, W. (2001) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(01)00137-1 · Cited by 1,176
Dietary Isoflavones: Biological Effects and Relevance to Human Health
Setchell, K. D. R., & Cassidy, A. (1999) · Journal of Nutrition · doi:10.1093/jn/129.3.758s · Cited by 1,163
Phytoestrogens: the biochemistry, physiology, and implications for human health of soy isoflavones
Setchell, K. D. R. (1998) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/68.6.1333s · Cited by 1,137
Rapid method for distinction of gram-negative from gram-positive bacteria
Gregersen, T. (1978) · Applied Microbiology and Biotechnology · doi:10.1007/bf00498806 · Cited by 948
Interaction of Phytoestrogens with Estrogen Receptors .ALPHA. and .BETA..
Morito, K., HIROSE, T., Kinjo, J., Hirakawa, T., Okawa, M., Nohara, T., Ogawa, S., Inoue, S., Muramatsu, M., & Masamune, Y. (2001) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.24.351 · Cited by 622
Rowland, I. R., Wiseman, H., Sanders, T. A. B., Adlercreutz, H., & Bowey, E. A. (2000) · Nutrition and Cancer · doi:10.1207/s15327914nc3601_5 · Cited by 583
Gut Bacterial Metabolism of the Soy Isoflavone Daidzein: Exploring the Relevance to Human Health
Atkinson, C., Frankenfeld, C. L., & Lampe, J. W. (2005) · Experimental Biology and Medicine · doi:10.1177/153537020523000302 · Cited by 516
Setchell, K. D., Clerici, C., Lephart, E. D., Cole, S. J., Heenan, C., Castellani, D., Wolfe, B. E., Nechemias-Zimmer, L., Brown, N. M., Lund, T. D., et al. (2005) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/81.5.1072 · Cited by 471
Cited by
Sorted by citations
Panche, A. N., Diwan, A. D., & Chandra, S. (2016) · Journal of Nutritional Science · doi:10.1017/jns.2016.41 · Cited by 5,953
Human Gut Microbiota from Autism Spectrum Disorder Promote Behavioral Symptoms in Mice
Sharon, G., Cruz, N. J., Kang, D.-W., Gandal, M. J., Wang, B., Kim, Y.-M., Zink, E. M., Casey, C. P., Taylor, B. C., Lane, C. J., et al. (2019) · Cell · doi:10.1016/j.cell.2019.05.004 · Cited by 1,182
Equol: History, Chemistry, and Formation
Setchell, K. D. R., & Clerici, C. (2010) · The Journal of Nutrition · doi:10.3945/jn.109.119776 · Cited by 519
Isoflavones: estrogenic activity, biological effect and bioavailability
Vitale, D. C., Piazza, C., Melilli, B., Drago, F., & Salomone, S. (2012) · European Journal of Drug Metabolism and Pharmacokinetics · doi:10.1007/s13318-012-0112-y · Cited by 500
The Interactions between Polyphenols and Microorganisms, Especially Gut Microbiota
Makarewicz, M., Drożdż, I., Tarko, T., & Duda‐Chodak, A. (2021) · Antioxidants · doi:10.3390/antiox10020188 · Cited by 466
Metadata
- Authors
- Matthies, A., Clavel, T., Gütschow, M., Engst, W., Haller, D., Blaut, M., & Braune, A.
- Affiliations
- German Institute of Human Nutrition (DE)
- Technical University of Munich (DE)
- Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany
- Department of Gastrointestinal Microbiology
- Corresponding author
- Braune, Annett
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-08
- Volume
- 74
- Issue
- 15
- Pages
- 4847-4852
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2519357
- OpenAlex
- W2067334985
- Cited by
- 141
- References
- 57
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 16 · 2023: 9 · 2022: 15 · 2021: 9 · 2020: 10 · 2019: 11 · 2018: 5 · 2017: 5 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 8 · 2012: 6
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Phytoestrogen effects and research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- daidzein, genistein, isoflavone metabolism, equol, dihydrodaidzein, gut microbiota, Coriobacteriaceae, anaerobic bacteria, enzyme induction, soy isoflavones, mouse intestine, metabolic activation
- MeSH terms
- Animals, Bacteria, Anaerobic, Biotransformation, Chromatography, High Pressure Liquid, Female, Gram-Positive Bacteria, Ileum, Isoflavones, Mice, Inbred C57BL, Molecular Sequence Data, Genistein, Spectrometry, Mass, Electrospray Ionization, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
