The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
Open accessAbstract
This work describes novel genetic tools for use in Clostridium thermocellum that allow creation of unmarked mutations while using a replicating plasmid. The strategy employed counter-selections developed from the native C. thermocellum hpt gene and the Thermoanaerobacterium saccharolyticum tdk gene and was used to delete the genes for both lactate dehydrogenase (Ldh) and phosphotransacetylase (Pta). The Δ ldh Δ pta mutant was evolved for 2,000 h, resulting in a stable strain with 40:1 ethanol selectivity and a 4.2-fold increase in ethanol yield over the wild-type strain. Ethanol production from cellulose was investigated with an engineered coculture of organic acid-deficient engineered strains of both C. thermocellum and T. saccharolyticum . Fermentation of 92 g/liter Avicel by this coculture resulted in 38 g/liter ethanol, with acetic and lactic acids below detection limits, in 146 h. These results demonstrate that ethanol production by thermophilic, cellulolytic microbes is amenable to substantial improvement by metabolic engineering.
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Cites
Microbial Cellulose Utilization: Fundamentals and Biotechnology
Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.506-577.2002 · Cited by 4,726
Self-citationHow biotech can transform biofuels
Lynd, L. R., Laser, M. S., Bransby, D., Dale, B. E., Davison, B., Hamilton, R., Himmel, M., Keller, M., McMillan, J. D., Sheehan, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt0208-169 · Cited by 1,121
Self-citationCellulase, Clostridia, and Ethanol
Demain, A. L., Newcomb, M., & Wu, J. H. D. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.1.124-154.2005 · Cited by 904
The prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass
Maki, M., Leung, K. T., & Qin, W. (2009) · International Journal of Biological Sciences · doi:10.7150/ijbs.5.500 · Cited by 559
Shanks, R. M. Q., Caiazza, N. C., Hinsa, S. M., Toutain, C. M., & O'Toole, G. A. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.00682-06 · Cited by 390
Self-citationEngineering for biofuels: exploiting innate microbial capacity or importing biosynthetic potential?
Alper, H., & Stephanopoulos, G. (2009) · Nature Reviews Microbiology · doi:10.1038/nrmicro2186 · Cited by 389
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
Shaw, A. J., Podkaminer, K. K., Desai, S. G., Bardsley, J. S., Rogers, S. R., Thorne, P. G., Hogsett, D. A., & Lynd, L. R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801266105 · Cited by 388
Self-citationFrom cellulosomes to cellulosomics
Bayer, E. A., Lamed, R., White, B. A., & Flint, H. J. (2008) · The Chemical Record · doi:10.1002/tcr.20160 · Cited by 322
HPRT: GENE STRUCTURE, EXPRESSION, AND MUTATION
Stout, J. T., & Caskey, C. T. (1985) · Annual Review of Genetics · doi:10.1146/annurev.ge.19.120185.001015 · Cited by 228
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Commercial Biomass Syngas Fermentation.
Daniell, J., Köpke, M., & Simpson, S. (2012) · Energies · doi:10.3390/en5125372 · Cited by 444
Salehi Jouzani, G., & Taherzadeh, M. J. (2015) · Biofuel Research Journal · doi:10.18331/brj2015.2.1.4 · Cited by 224
Jiang, Y., Wu, R., Zhou, J., He, A., Xu, J., Xin, F., Zhang, W., Ma, J., Jiang, M., & Dong, W. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1495-7 · Cited by 93
Technical guide for genetic advancement of underdeveloped and intractable Clostridium
Pyne, M. E., Bruder, M., Moo-Young, M., Chung, D. A., & Chou, C. P. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.04.003 · Cited by 78
Metadata
- Authors
- Argyros, D. A., Tripathi, S. A., Barrett, T. F., Rogers, S. R., Feinberg, L. F., Olson, D. G., Foden, J. M., Miller, B. B., Lynd, L. R., Hogsett, D. A., & Caiazza, N. C.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2011-12
- Volume
- 77
- Issue
- 23
- Pages
- 8288-8294
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2131923812
- Cited by
- 334
- References
- 22
- FWCI
- 12.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Argyros, D. A., Tripathi, S. A., Barrett, T. F., Rogers, S. R., Feinberg, L. F., Olson, D. G., Foden, J. M., Miller, B. B., Lynd, L. R., Hogsett, D. A., & Caiazza, N. C. (2011). High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.00646-11
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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