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Duodenal-Mucosal Bacteria Associated with Celiac Disease in Children
Open accessAbstract
Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cultivable duodenal mucosa-associated bacteria of CD patients and control children. Duodenal biopsy specimens from patients with active disease on a gluten-containing diet (n= 32), patients with nonactive disease after adherence to a gluten-free diet (n= 17), and controls (n= 8) were homogenized and plated on plate count agar, Wilkins-Chalgren agar, brain heart agar, or yeast, Casitone, and fatty acid agar. The isolates were identified by partial 16S rRNA gene sequencing. Renyi diversity profiles showed the highest diversity values for active CD patients, followed by nonactive CD patients and control individuals. Members of the phylum Proteobacteria were more abundant in patients with active CD than in the other child groups, while those of the phylum Firmicutes were less abundant. Members of the families Enterobacteriaceae and Staphylococcaceae, particularly the species Klebsiella oxytoca,Staphylococcus epidermidis, and Staphylococcus pasteuri, were more abundant in patients with active disease than in controls. In contrast, members of the family Streptococcaceae were less abundant in patients with active CD than in controls. Furthermore, isolates of the Streptococcus anginosus and Streptococcus mutans groups were more abundant in controls than in both CD patient groups, regardless of inflammatory status. The findings indicated that the disease is associated with the overgrowth of possible pathobionts that exclude symbionts or commensals that are characteristic of the healthy small intestinal microbiota.
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“Most studies, but not all,48 have found significant differences between healthy children and children with CD in the duodenal49,50 and oral51 microbial populations.”
From The Gut Microbiome and the Brain (2014) · cites this paper as [49]
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Metadata
- Authors
- Sánchez, E., Donat, E., Ribes-Koninckx, C., Fernández-Murga, M. L., & Sanz, Y.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- Hospital Universitari i Politècnic La Fe (ES)
- Hospital Universitario La Fe, Valencia, Spain
- Microbial Ecology and Nutrition Research Group, Institute of Agrochemistry and Food Technology, National Research Council (IATA-CSIC), Valencia, Spain
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2013-09-15
- Volume
- 79
- Issue
- 18
- Pages
- 5472-5479
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC3754165
- OpenAlex
- W2127055805
- Cited by
- 171
- References
- 55
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 17 · 2023: 10 · 2022: 10 · 2021: 18 · 2020: 18 · 2019: 18 · 2018: 11 · 2017: 12 · 2016: 17 · 2015: 12 · 2014: 15 · 2013: 1
- FWCI
- 6.72
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Danone
- Ministerio de Economía y Competitividad
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Gastroenterology › Celiac Disease Research and Management
- Health Sciences › Medicine › Gastroenterology › Helicobacter pylori-related gastroenterology studies
- Keywords
- celiac disease, duodenal mucosa, gut microbiota, cultivable bacteria, pathobionts, Klebsiella oxytoca, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus anginosus, Proteobacteria, Firmicutes, 16S rRNA gene sequencing, gluten-free diet, children, microbial diversity
- MeSH terms
- Bacteria, Bacteriological Techniques, Biopsy, Celiac Disease, Child, Child, Preschool, DNA, Bacterial, DNA, Ribosomal, Duodenum, Humans, Intestinal Mucosa, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Biodiversity
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