Secretion of insulinotropic proteins by commensal bacteria: rewiring the gut to treat diabetes
Open accessAbstract
Here, we show that commensal bacteria can stimulate intestinal epithelial cells to secrete insulin in response to glucose. Commensal strains were engineered to secrete the insulinotropic proteins GLP-1 and PDX-1. Epithelia stimulated by engineered strains and glucose secreted up to 1 ng ml(-1) of insulin with no significant background secretion.
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Microbial manipulations and supplementation of microbial metabolites are being considered as emerging preventive and therapeutic medicines for numerous disorders including those with gut barrier dysfunction and dysbiosis.
“Microbial manipulations and supplementation of microbial metabolites are being considered as emerging preventive and therapeutic medicines for numerous disorders185, 186, 187 including those with gut barrier dysfunction and dysbiosis.”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [187]
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Biology of Incretins: GLP-1 and GIP
Baggio, L. L., & Drucker, D. J. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.03.054 · Cited by 3,639 · see citing works
Hansen, L., Deacon, C. F., Ørskov, C., & Holst, J. J. (1999) · Endocrinology · doi:10.1210/endo.140.11.7143 · Cited by 595 · see citing works
Effects of Age and Sex on Postprandial Glucose Metabolism
Basu, R., Dalla Man, C., Campioni, M., Basu, A., Klee, G., Toffolo, G., Cobelli, C., & Rizza, R. A. (2006) · Diabetes · doi:10.2337/db05-1692 · Cited by 334 · see citing works
Wittmann, C. (2007) · Microbial Cell Factories · doi:10.1186/1475-2859-6-6 · Cited by 225 · see citing works
Glucagon-like peptide 1 (1–37) converts intestinal epithelial cells into insulin-producing cells
Suzuki, A., Nakauchi, H., & Taniguchi, H. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0936260100 · Cited by 140 · see citing works
PDX-1 Induces Differentiation of Intestinal Epithelioid IEC-6 Into Insulin-Producing Cells
Yoshida, S., Kajimoto, Y., Yasuda, T., Watada, H., Fujitani, Y., Kosaka, H., Gotow, T., Miyatsuka, T., Umayahara, Y., Yamasaki, Y., et al. (2002) · Diabetes · doi:10.2337/diabetes.51.8.2505 · Cited by 127 · see citing works
Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency
Liang, J. F., & Yang, V. C.-M. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.07.142 · Cited by 124 · see citing works
Daniel, C., Repa, A., Wild, C., et al. (2006) · Allergy · doi:10.1111/j.1398-9995.2006.01071.x · Cited by 108 · see citing works
Genetically modified probiotics in foods
Ahmed, F. (2003) · Trends in biotechnology · doi:10.1016/j.tibtech.2003.09.006 · Cited by 107 · see citing works
Majander, K., Anton, L., Antikainen, J., Lång, H. A., Brummer, M. H., Korhonen, T., & Westerlund‐Wikström, B. (2005) · Nature Biotechnology · doi:10.1038/nbt1077 · Cited by 103 · see citing works
Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei
Hazebrouck, S., Pothelune, L., Azevedo, V., Corthier, G., Wal, J. M., & Langella, P. (2007) · Microbial Cell Factories · doi:10.1186/1475-2859-6-12 · Cited by 56 · see citing works
Farrar, M. D., Whitehead, T. R., Lan, J. Q., Dilger, P., Thorpe, R., Holland, K. T., & Carding, S. R. (2005) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2005.02565.x · Cited by 51 · see citing works
Duan, F., & March, J. C. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.21897 · Cited by 45 · see citing works
Self-citationKoizumi, M., Doi, R., Fujimoto, K., Ito, D., Toyoda, E., Mori, T., Kami, K., Kawaguchi, Y., Gittes, G. K., & Imamura, M. (2005) · Surgery · doi:10.1016/j.surg.2005.06.008 · Cited by 16 · see citing works
Forced expression of PDX-1 induces insulin production in intestinal epithelia
Koizumi, M., Nagai, K., Kida, A., et al. (2006) · Surgery · doi:10.1016/j.surg.2006.06.014 · Cited by 16 · see citing works
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Research works printed but not linked: 1
- [8]Hartmann, M., A. Westendorf, J. Buer, and F. Gunzer. 2004. E-coli Nissle 1917 as a vehicle for intestinal expression of therapeutic molecules: construction of an E-coli a hemolysin based expression vector. Int. J. Med. Microbiol. 294:198. [Google Scholar]
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The Human Intestinal Microbiome in Health and Disease
Lynch, S. V., & Pedersen, O. (2016) · New England Journal of Medicine · doi:10.1056/nejmra1600266 · Cited by 3,637 · see citing works
Regulation of Intestinal Barrier Function by Microbial Metabolites
Ghosh, S., Whitley, C. S., Haribabu, B., & Jala, V. R. (2021) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2021.02.007 · Cited by 835 · see citing works
Gut microbiota, obesity and diabetes
Patterson, E., Ryan, P. M., Cryan, J. F., Dinan, T. G., Ross, R. P., Fitzgerald, G. F., & Stanton, C. (2016) · Postgraduate Medical Journal · doi:10.1136/postgradmedj-2015-133285 · Cited by 552 · see citing works
Role of the gut microbiota in type 2 diabetes and related diseases
Yang, G., Wei, J., Liu, P., Zhang, Q., Tian, Y., Hou, G., Meng, L., Xin, Y., & Jiang, X. (2021) · Metabolism · doi:10.1016/j.metabol.2021.154712 · Cited by 418 · see citing works
Duan, F. F., Liu, J. H., & March, J. C. (2015) · Diabetes · doi:10.2337/db14-0635 · Cited by 263 · see citing works
Self-citationThe non-pathogenic <i>Escherichia coli</i> strain Nissle 1917 – features of a versatile probiotic
Sonnenborn, U., & Schulze, J. (2009) · Microbial Ecology in Health and Disease · doi:10.3109/08910600903444267 · Cited by 232 · see citing works
- Authors
- Duan, F., Curtis, K. L., & March, J. C.
- Affiliations
- Cornell University (US)
- ORCID
- March, John C.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-12
- Volume
- 74
- Issue
- 23
- Pages
- 7437-7438
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2592943
- OpenAlex
- W2099325141
- Cited by
- 86
- References
- 15
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 10 · 2019: 4 · 2018: 6 · 2017: 9 · 2016: 7 · 2015: 5 · 2014: 9 · 2013: 2 · 2012: 7
- FWCI
- 1.37
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (DP2OD007155)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Keywords
- commensal bacteria, insulin secretion, glucagon-like peptide-1, PDX1, engineered bacteria, intestinal epithelial cells, glucose stimulation, diabetes treatment, gut microbiota, insulinotropic peptides
- MeSH terms
- Insulin Secretion, Epithelial Cells, Escherichia coli, Humans, Insulin, Recombinant Proteins, Trans-Activators, Homeodomain Proteins, Caco-2 Cells, Glucagon-Like Peptide 1, Incretins
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