The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Lactose-Inducible System for Metabolic Engineering of Clostridium ljungdahlii
Open accessAbstract
The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic physiology of acetogenesis. In an attempt to expand the genetic toolbox for C. ljungdahlii, the possibility of adapting a lactose-inducible system for gene expression, previously reported for Clostridium perfringens, was investigated. The plasmid pAH2, originally developed for C. perfringens with a gusA reporter gene, functioned as an effective lactose-inducible system in C. ljungdahlii. Lactose induction of C. ljungdahlii containing pB1, in which the gene for the aldehyde/alcohol dehydrogenase AdhE1 was downstream of the lactose-inducible promoter, increased expression of adhE1 30-fold over the wild-type level, increasing ethanol production 1.5-fold, with a corresponding decrease in acetate production. Lactose-inducible expression of adhE1 in a strain in which adhE1 and the adhE1 homolog adhE2 had been deleted from the chromosome restored ethanol production to levels comparable to those in the wild-type strain. Inducing expression of adhE2 similarly failed to restore ethanol production, suggesting that adhE1 is the homolog responsible for ethanol production. Lactose-inducible expression of the four heterologous genes necessary to convert acetyl coenzyme A (acetyl-CoA) to acetone diverted ca. 60% of carbon flow to acetone production during growth on fructose, and 25% of carbon flow went to acetone when carbon monoxide was the electron donor. These studies demonstrate that the lactose-inducible system described here will be useful for redirecting carbon and electron flow for the biosynthesis of products more valuable than acetate. Furthermore, this tool should aid in optimizing microbial electrosynthesis and for basic studies on the physiology of acetogenesis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Gene expression reporter systems that function under anaerobic conditions have emerged, including the β-glucuronidase gene (gusA) (Girbal et al., 2003; Dong et al., 2012; Banerjee et al., 2014), chloramphenicol acetyltransferase gene (catP) (Heap et al., 2007; Zhang et al., 2015b), flavin-based fluorescent proteins (Drepper et al., 2007, 2010; Mukherjee and Schroeder, 2015), luxA-luxB reporter”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Banerjee et al., 2014
In the citing paper
“A lactose-inducible system (bgaR-PbgaL) from Clostridium perfringens was successfully adapted for use in C. ljungdahlii (Banerjee et al., 2014) and a tetracycline inducible system (Tet3no) has been demonstrated in C. autoethanogenum (Walker and Köpke, 2015).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Banerjee et al., 2014
In the citing paper
“| C. ljungdahlii | Plasmid expression of C. acetobutylicum acetone biosynthetic genes (thlA-ctfAB-adc) under lactose inducible promoter | Production of up to 871 mg/L acetone from CO in bottles | Banerjee et al., 2014 |”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Banerjee et al., 2014
In the citing paper
“Acetone production increased over 500x from H2/CO2 in C. ljungdahlii using the same genes but under control of an inducible system (Banerjee et al., 2014) and in A. woodii by combinatorial testing of different promoter and plasmid combinations (Hoffmeister et al., 2016).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Banerjee et al., 2014
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Acetone-butanol fermentation revisited
Jones, D. T., & Woods, D. (1986) · Microbiological Reviews · doi:10.1128/mr.50.4.484-524.1986 · Cited by 1,966
Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation
Ragsdale, S. W., & Pierce, E. (2008) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2008.08.012 · Cited by 1,409
Nevin, K. P., Woodard, T. L., Franks, A. E., Summers, Z. M., & Lovley, D. R. (2010) · mBio · doi:10.1128/mbio.00103-10 · Cited by 1,068
Self-citationNevin, K. P., Hensley, S. A., Franks, A. E., Summers, Z. M., Ou, J., Woodard, T. L., Snoeyenbos-West, O., & Lovley, D. R. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.02642-10 · Cited by 722
Self-citationThe ClosTron: a universal gene knock-out system for the genus Clostridium
Heap, J. T., Pennington, O. J., Cartman, S. T., Carter, G. P., & Minton, N. P. (2007) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2007.05.021 · Cited by 663
Clostridium ljungdahlii represents a microbial production platform based on syngas
Köpke, M., Held, C., Hujer, S., Liesegang, H., Wiezer, A., Wollherr, A., Ehrenreich, A., Liebl, W., Gottschalk, G., & Dürre, P. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1004716107 · Cited by 660
Potential for Direct Interspecies Electron Transfer in Methanogenic Wastewater Digester Aggregates
Morita, M., Malvankar, N. S., Franks, A. E., Summers, Z. M., Giloteaux, L., Rotaru, A., Rotaru, C., & Lovley, D. R. (2011) · mBio · doi:10.1128/mbio.00159-11 · Cited by 575
Self-citationKöpke, M., Mihalcea, C., Liew, F. E., Tizard, J. H., Ali, M., Conolly, J. J., Al‐Sinawi, B., & Simpson, S. D. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.00355-11 · Cited by 418
Tracy, B. P., Jones, S. W., Fast, A. G., Indurthi, D. C., & Papoutsakis, E. T. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.10.008 · Cited by 408
Enzymology of the Wood–Ljungdahl Pathway of Acetogenesis
Ragsdale, S. W. (2008) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1419.015 · Cited by 405
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–126 of 126
Genetic sensor-regulators functional in Clostridia
Han, S., Kim, Y., Karanjikar, M., San, K., & Bennett, G. N. (2020) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-020-02303-6 · Cited by 2
Woods, C., Humphreys, C. M., Tomi-Andrino, C., Henstra, A. M., Köpke, M., Simpson, S. D., Winzer, K., & Minton, N. P. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.05.19.444907 · Cited by 3
Flaiz, M., Ludwig, G., Bengelsdorf, F. R., & Dürre, P. (2021) · Research Square · doi:10.21203/rs.3.rs-171102/v1 · Cited by 2
Baur, K. S., Stock, A., Weuster‐Botz, D., & Bengelsdorf, F. R. (2026) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-026-13952-5 · Cited by 1
Microbial electrosynthesis: CO2 fixation driven by electricity
ZHANG, Y., Yin, L. I., & Zhu, H. (2016) · Scientia Sinica Vitae · doi:10.1360/n052016-00226 · Cited by 1
Woo, J. E., Song, S. M., Lee, S. Y., & Jang, Y. (2017) · Springer eBooks · doi:10.1007/978-3-319-31421-1_389-1 · Cited by 1
Woo, J. E., Song, S. M., Lee, S. Y., & Jang, Y. (2017) · Springer eBooks · doi:10.1007/978-3-319-50436-0_389 · Cited by 1
Engineering Acetobacterium wieringae for acetone production from syngas
Moreira, J. P. C., Montenegro-Silva, P., Alves, J. I., & Domingues, L. (2024) · Journal of environmental chemical engineering · doi:10.1016/j.jece.2024.114980 · Cited by 1
Metabolic Engineering and the Synthetic Biology Toolbox forClostridium
Joseph, R. C., Kelley, S. Q., Kim, N. M., & Sandoval, N. R. (2021) · Metabolic Engineering · doi:10.1002/9783527823468.ch16 · Cited by 1
Wen, Z., & Jarboe, L. R. (2019) · doi:10.1002/9781119417637.ch9 · Cited by 1
Im, C., Krige, A., Valgepea, K., Modin, O., Nygård, Y., & Franzén, C. J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.09.12.675768 · Cited by 1
Metabolism and cell-to-cell interactions of anaerobic syntrophic Clostridia co-cultures
Streett, H. (2025) · Library, Museums and Press - UDSpace (University of Delaware) · doi:10.58088/pe3w-rj36
Clostridial Genetics: Genetic Manipulation of the Pathogenic Clostridia
Kuehne, S. A., Rood, J. I., & Lyras, D. (2019) · ASM Press eBooks · doi:10.1128/9781683670131.ch57
Schwarz, F. M. (2022) · Publication Server of Goethe University Frankfurt am Main (Goethe University Frankfurt) · doi:10.21248/gups.68197
Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from COPreprint
Dykstra, J. C., van Oort, J., Yazdi, A. T., Vossen, E., Patinios, C., John van der, O. O. S. T., Sousa, D. Z., & Kengen, S. W. M. (2022) · Research Square · doi:10.21203/rs.3.rs-1914331/v1
Genome Editing in Bacillus Licheniformis: Current Approaches and Applications
Raphel, S., & Halami, P. M. (2024) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815165678124010011
Amazing attributes of acetogensPreprint
Poehlein, A., Zeldes, B. M., Flaiz, M., Böer, T., Lüschen, A., Höfele, F., Baur, K. S., Molitor, B., Kröly, C., Wang, M., et al. (2024) · SSRN Electronic Journal · doi:10.2139/ssrn.4872466
Lauer, I., Philipps, G., & Jennewein, S. (2022) · Figshare · doi:10.6084/m9.figshare.19767719
Flaiz, M., Ludwig, G., Bengelsdorf, F. R., & Dürre, P. (2021) · Figshare · doi:10.6084/m9.figshare.14570425
Dykstra, J. C., van Oort, J., Yazdi, A. T., Vossen, E., Patinios, C., John van der, O. O. S. T., Sousa, D. Z., & Kengen, S. W. M. (2022) · Figshare · doi:10.6084/m9.figshare.21622395
Moreira, J. P. C., Heap, J. T., Alves, J. I., & Domingues, L. (2024) · Figshare · doi:10.6084/m9.figshare.26568691
Kato, J., Takemura, K., Kato, S., Fujii, T., Wada, K., Iwasaki, Y., Aoi, Y., Matsushika, A., Murakami, K., & Nakashimada, Y. (2021) · Figshare · doi:10.6084/m9.figshare.14478669
Singhal, S., Gupta, J., Rani, V., Zainab, M., Kumar, M., Parida, K. M., & Giri, A. (2026) · 3 Biotech · doi:10.1007/s13205-026-04902-z
Eynard, R., Justine, S., Hocq, R., & Pflügl, S. (2026) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-026-02790-2
Dao, O. T., Woo, J. E., Lee, S. Y., & Jang, Y. (2026) · Springer eBooks · doi:10.1007/978-3-319-31421-1_389-2
A cryptic lactate cycle in CO fermentation by Clostridium ljungdahlii
Sun, H., Yi, J., Huang, H., & Wang, S. (2026) · Applied and Environmental Microbiology · doi:10.1128/aem.01142-26
Metadata
- Authors
- Banerjee, A., Leang, C., Ueki, T., Nevin, K. P., & Lovley, D. R.
- Affiliations
- University of Massachusetts Amherst (US)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2014-04-15
- Volume
- 80
- Issue
- 8
- Pages
- 2410-2416
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC3993169
- OpenAlex
- W2120580364
- Cited by
- 126
- References
- 34
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 7 · 2023: 5 · 2022: 16 · 2021: 15 · 2020: 7 · 2019: 13 · 2018: 7 · 2017: 12 · 2016: 10 · 2015: 14 · 2014: 4
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy (DEAR0000159)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Keywords
- Clostridium ljungdahlii, aldehyde/alcohol dehydrogenase, ethanol production, acetate production, acetone production, acetogenesis, syngas fermentation, microbial electrosynthesis
- MeSH terms
- Acetone, Acetyl Coenzyme A, Alcohol Dehydrogenase, Ethanol, Carbon, Clostridium, Fructose, Lactose, Transcriptional Activation, Gene Expression, Gene Expression Regulation, Bacterial, Acetic Acid, Metabolic Engineering, Metabolic Flux Analysis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
