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Cited by 91Bacterial Genetics and BiotechnologyEnzyme Production and CharacterizationProbiotics and Fermented Foods

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

Localization of Peptidases in Lactococci

Open access
Tan, Paris S. T., Chapot-Chartier, Marie-Pierre, Pos, Klaas M., Rousseau, Micheline, Boquien, Clair-Yves, Gripon, Jean-Claude, Konings, Wil N.·1992·Applied and Environmental Microbiology
DOI 10.1128/aem.58.1.285-290.1992PMC PMC195205
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Abstract

The localization of two aminopeptidases, an X-prolyl-dipeptidyl aminopeptidase, an endopeptidase, and a tripeptidase in Lactococcus lactis was studied. Polyclonal antibodies raised against each purified peptidase are specific and do not cross-react with other peptidases. Experiments were performed by immunoblotting after cell fractionation and by electron microscopy of immunogold-labeled peptidases. All peptidases were found to be intracellular. However, immunogold studies showed a peripheral labeling of the X-prolyl-dipeptidyl aminopeptidase, the tripeptidase, and the endopeptidase. This peripheral location was further supported by the detection of these three enzymes in cell membrane fractions in which none of the two aminopeptidases was present.

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Crossref · 10.1128/aem.58.1.285-290.1992

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44 of 44 references verified0 citation errors · 0 not checkable

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  • The aminopeptidases PepN and PepC, the X-prolyl-dipeptidyl aminopeptidase PepX, the endopeptidase PepO, the tripeptidase PepT, and the glutamyl aminopeptidase PepA are present inside the cell.

    “biochemical and immunological data indicate that the aminopeptidases (PepN and PepC), the Xprolyl-dipeptidyl aminopeptidase (PepX), the endopeptidase (PepO), the tripeptidase (Pep'I'), and the glutamyl aminopeptidase (PepA) are present inside the cell (Tan et al., 1992;Baankreis, 1992).”

    From Transport of β-Casein-derived Peptides by the Oligopeptide Transport System Is a Crucial Step in the Proteolytic Pathway of Lactococcus lactis (1995) · cites this paper as Tan et al., 1992

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Metadata

Authors
Tan, P. S. T., Chapot-Chartier, M.-P., Pos, K. M., Rousseau, M., Boquien, C.-Y., Gripon, J.-C., & Konings, W. N.
Affiliations
  • University of Groningen (NL)
  • Department of Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands, and Station de Recherches Laitieres, Institute National de la Recherche Agronomique, 78350 Jouy-en-Josas, 2 and Laboratoire de Génie des Procédés Biotechnologiques Agro-alimentaires, Institute National de la Recherche Agronomique, 7850 Thiverval-Grignon, 3 France
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
1992-01
Volume
58
Issue
1
Pages
285-290
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.58.1.285-290.1992
PMC
PMC195205
OpenAlex
W2135291905
Cited by
91
References
44
Citations by year
2025: 2 · 2020: 2 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 1 · 2012: 3
FWCI
7.20
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
Lactococcus lactis, aminopeptidase, X-prolyl dipeptidyl aminopeptidase, endopeptidase, tripeptidase, intracellular enzymes, plasma membrane, immunogold labeling, polyclonal antibodies

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