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Cited by 85Bacterial Genetics and BiotechnologyProbiotics and Fermented FoodsBacteriophages and microbial interactions

Autolysis of Lactococcus lactis caused by induced overproduction of its major autolysin AcmA

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Buist, G, Karsens, H, Nauta, A, van Sinderen, D, Venema, G, Kok, J·1997·Applied and Environmental Microbiology
DOI 10.1128/aem.63.7.2722-2728.1997PMC PMC168568
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Abstract

The optical density of a culture of lactococcus lactis MG1363 was reduced more than 60% during prolonged stationary phase. Reduction in optical density (autolysis) was almost absent in a culture of an isogenic mutant containing a deletion in the major autolysin gene, acmA. An acmA mutant carrying multiple coples of a plasmid encoding AcmA lysed to a greater extent than the wild-type strain did. Intercellular action of AcmA was shown by mixing end-exponential-phase cultures of an acmA deletion mutant and a tripeptidase (pepT) deletion mutant. PepT, produced by the acmA mutant, was detected in the supernatant of the mixed culture, but no PepT was present in the culture supernatant of the acmA mutant. A plasmid was constructed in which acmA, lacking its own promoter, was placed downstream of the inducible promoter/operator region of the temperate lactococcal bacteriophage r1t. After mitomycin induction of an exponential-phase culture of L. lactis LL302 carrying this plasmid, the cells became subject to autolysis, resulting in the release of intracellular proteins.

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  • Deletion of the autolysin enabled prolonging incubation times of cells in buffer up to 6 h without detectable release of intracellular peptidases.

    “Deletion of the autolysin enabled us to prolong the incubation times of cells in buffer up to 6 h without detectable release of intracellular peptidases (Buist et al., 1997).”

    From Reconstruction of the proteolytic pathway for use of β‐casein by <i>Lactococcus lactis</i> (1998) · cites this paper as Buist et al., 1997

    The Discussion reports that deleting acmA eliminated autolysis and that the mutant did not release intracellular proteins. The results also report no detectable PepT in the mutant’s culture supernatant. However, these observations were made in culture medium; the paper does not establish the claimed six-hour incubation in buffer. (LLM, conf=0.88)

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Research works printed but not linked: 2

  1. [29]29. Østlie, H. M. 1995. Autolysis of lactococci and dairy propionbacteria. Ph.D. thesis. Agricultural University of Norway, Ås.
  2. [31]31. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

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Authors
Buist, G., Karsens, H., Nauta, A., van Sinderen, D., Venema, G., & Kok, J.
Affiliations
  • University of Groningen (NL)
  • Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
1997-07
Volume
63
Issue
7
Pages
2722-2728
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.63.7.2722-2728.1997
PMC
PMC168568
OpenAlex
W2125621374
Cited by
84
References
47
Citations by year
2026: 1 · 2025: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2016: 4 · 2015: 6 · 2014: 4 · 2013: 2 · 2012: 4
FWCI
2.44
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
Keywords
Lactococcus lactis, autolysis, tripeptidase, plasmid overexpression, intracellular protein release
MeSH terms
Aminopeptidases, Bacteriolysis, Bacteriophages, Chromosome Mapping, Genetic Complementation Test, Mitomycins, Muramidase, Plasmids, Promoter Regions, Genetic, Recombination, Genetic, Lactococcus lactis, Transformation, Genetic, Gene Expression, S Phase, Sequence Deletion

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