The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells
Open accessAbstract
The influence of pH on the adhesion of two Lactobacillus strains to Caco-2 human intestinal cells was investigated. One strain, Lactobacillus johnsonii La1, was adherent at any pH between 4 and 7. The other one, L. acidophilus La10, did not attach to this cell line under the same experimental conditions. On the basis of these results, we used the monoclonal antibody technique as a tool to determine differences on the surface of these bacteria and to identify a factor for adhesion. Mice were immunized with live La1, and the hybridomas produced by fusion of spleen cells with ONS1 cells were screened for the production of antibodies specific for L. johnsonii La1. A set of these monoclonal antibodies was directed against a nonproteinaceous component of the L. johnsonii La1 surface. It was identified as lipoteichoic acid (LTA). This molecule was isolated, chemically characterized, and tested in adhesion experiments in the same system. The adhesion of L. johnsonii La1 to Caco-2 cells was inhibited in a concentration-dependent way by purified LTA as well as by L. johnsonii La1 culture supernatant that contained LTA. These results showed that the mechanism of adhesion of L. johnsonii La1 to human Caco-2 cells involves LTA.
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Cites
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Hudault, S., Liévin, V, Bernet-Camard, M. F., & Servin, A. L. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.2.513-518.1997 · Cited by 329 · cited 1×
“casei GG were recently shown to have antipathogenic effects either in cellular or in animal models (7, 8, 31).”
BackgroundAdhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells
CHAUVIERE, G., COCONNIER, M.-H., KERNEIS, S., FOURNIAT, J., & SERVIN, A. L. (1992) · Journal of General Microbiology · doi:10.1099/00221287-138-8-1689 · Cited by 249
Self-citation
Cited by
Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens
Servin, A. L. (2004) · FEMS Microbiology Reviews · doi:10.1016/j.femsre.2004.01.003 · Cited by 1,236
Self-citationGenes and Molecules of Lactobacilli Supporting Probiotic Action
Lebeer, S., Vanderleyden, J., & De Keersmaecker, S. C. J. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00017-08 · Cited by 1,013
Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum
Del Re, B., Sgorbati, B., Miglioli, M., & Palenzona, D. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1365-2672.2000.00845.x · Cited by 731
The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533
Pridmore, R. D., Berger, B., Desiere, F., Vilanova, D., Barretto, C., Pittet, A.-C., Zwahlen, M.-C., Rouvet, M., Altermann, E., Barrangou, R., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0307327101 · Cited by 485
Self-citationGrangette, C., Nutten, S., Palumbo, E., Morath, S., Hermann, C., Dewulf, J., Pot, B., Hartung, T., Hols, P., & Mercenier, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504084102 · Cited by 443
Metadata
- Authors
- Granato, D., Perotti, F., Masserey, I., Rouvet, M., Golliard, M., Servin, A., & Brassart, D.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1999-03
- Volume
- 65
- Issue
- 3
- Pages
- 1071-1077
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2116839692
- Cited by
- 250
- References
- 3
- FWCI
- 17.74
- Type
- journal article
- Language
- en
- Open access
- green
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- Granato, D., Perotti, F., Masserey, I., Rouvet, M., Golliard, M., Servin, A., & Brassart, D. (1999). Cell Surface-Associated Lipoteichoic Acid Acts as an Adhesion Factor for Attachment of Lactobacillus johnsonii La1 to Human Enterocyte-Like Caco-2 Cells. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.65.3.1071-1077.1999
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
