The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Quorum-Sensing Genes in Pseudomonas aeruginosa Biofilms: Their Role and Expression Patterns
Open accessAbstract
Acylated homoserine lactone molecules are used by a number of gram-negative bacteria to regulate cell density-dependent gene expression by a mechanism known as quorum sensing (QS). In Pseudomonas aeruginosa , QS or cell-to-cell signaling controls expression of a number of virulence factors, as well as biofilm differentiation. In this study, we investigated the role played by the las and rhl QS systems during the early stages of static biofilm formation when cells are adhering to a surface and forming microcolonies. These studies revealed a marked difference in biofilm formation between the PAO1 parent and the QS mutants when glucose, but not citrate, was used as the sole carbon source. To further elucidate the contribution of lasI and rhlI to biofilm maturation, we utilized fusions to unstable green fluorescent protein in concert with confocal microscopy to perform real-time temporal and spatial studies of these genes in a flowing environment. During the course of 8-day biofilm development, lasI expression was found to progressively decrease over time. Conversely, rhlI expression remained steady throughout biofilm development but occurred in a lower percentage of cells. Spatial analysis revealed that lasI and rhlI were maximally expressed in cells located at the substratum and that expression decreased with increasing biofilm height. Because QS was shown previously to be involved in biofilm differentiation, these findings have important implications for the design of biofilm prevention and eradication strategies.
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Cited by
Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
Klausen, M., Heydorn, A., Ragas, P., Lambertsen, L., Aaes‐Jørgensen, A., Molin, S., & Tolker‐Nielsen, T. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03525.x · Cited by 1,020
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Strategies for culture of ‘unculturable’ bacteria: Culturing the unculturable
Vartoukian, S. R., Palmer, R. M., & Wade, W. G. (2010) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2010.02000.x · Cited by 718
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Quorum sensing and swarming migration in bacteria
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Metadata
- Authors
- De Kievit, T. R., Gillis, R., Marx, S., Brown, C., & Iglewski, B. H.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-04
- Volume
- 67
- Issue
- 4
- Pages
- 1865-1873
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2104768590
- Cited by
- 527
- References
- 28
- FWCI
- 8.58
- Type
- journal article
- Language
- en
- Open access
- green
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