Microbial Diversity of the Brine-Seawater Interface of the Kebrit Deep, Red Sea, Studied via 16S rRNA Gene Sequences and Cultivation Methods
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The brine-seawater interface of the Kebrit Deep, northern Red Sea, was investigated for the presence of microorganisms using phylogenetic analysis combined with cultivation methods. Under strictly anaerobic culture conditions, novel halophiles were isolated. The new rod-shaped isolates belong to the halophilic genus Halanaerobium and are the first representatives of the genus obtained from deep-sea, anaerobic brine pools. Within the genus Halanaerobium , they represent new species which grow chemoorganotrophically at NaCl concentrations ranging from 5 to 34%. The cellular fatty acid compositions are consistent with those of other Halanaerobium representatives, showing unusually large amounts of Δ7 and Δ11 16:1 fatty acids. Phylogenetic analysis of the brine-seawater interface sample revealed the presence of various bacterial 16S rRNA gene sequences dominated by cultivated members of the bacterial domain, with the majority affiliated with the genus Halanaerobium . The new Halanaerobium 16S rRNA clone sequences showed the highest similarity (99.9%) to the sequence of isolate KT-8-13 from the Kebrit Deep brine. In this initial survey, our polyphasic approach demonstrates that novel halophiles thrive in the anaerobic, deep-sea brine pool of the Kebrit Deep, Red Sea. They may contribute significantly to the anaerobic degradation of organic matter enriched at the brine-seawater interface.
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Short-chained hydrocarbons are formed by the degradation of long-chained hydrocarbons that originate from organic-rich sedimentary rocks and the bioproduction in the brine's water to sediment interface.
“Short-chained hydrocarbons are formed by the degradation of long-chained hydrocarbons that originate from the organic-rich sedimentary rocks34 and the bioproduction in the brine’s water to sediment interface35,36.”
From The Red Sea Deep Water is a potent source of atmospheric ethane and propane (2020) · cites this paper as [36]
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- Authors
- Eder, W., Jahnke, L. L., Schmidt, M., & Huber, R.
- Affiliations
- University of Regensburg (DE)
- Ames Research Center (US)
- Christian-Albrechts-Universität zu Kiel (DE)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-07
- Volume
- 67
- Issue
- 7
- Pages
- 3077-3085
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC92984
- OpenAlex
- W2072952450
- Cited by
- 140
- References
- 72
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 8 · 2019: 11 · 2018: 10 · 2017: 3 · 2016: 7 · 2015: 8 · 2014: 12 · 2013: 4 · 2012: 9
- FWCI
- 5.09
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
- University of Regensburg Publication Server (University of Regensburg) (acceptedVersion)
- Funders
- Universität Regensburg
- Institute of Genetics
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- halophiles, Halanaerobium, novel bacterial species, 16S rRNA gene sequences, cellular fatty acid composition, Red Sea
- MeSH terms
- Bacteria, Anaerobic, DNA, Ribosomal, Fatty Acids, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Seawater, Sodium Chloride, Sequence Analysis, DNA, Ecosystem, Genes, rRNA
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