The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Novel Cassette-Based Shuttle Vector System for Gram-Positive Bacteria
Open accessAbstract
Our understanding of staphylococcal pathogenesis depends on reliable genetic tools for gene expression analysis and tracing of bacteria. Here, we have developed and evaluated a series of novel versatile Escherichia coli -staphylococcal shuttle vectors based on PCR-generated interchangeable cassettes. Advantages of our module system include the use of (i) staphylococcal low-copy-number, high-copy-number, thermosensitive and theta replicons and selectable markers (choice of erythromycin, tetracycline, chloramphenicol, kanamycin, or spectinomycin); (ii) an E. coli replicon and selectable marker (ampicillin); and (iii) a staphylococcal phage fragment that allows high-frequency transduction and an SaPI fragment that allows site-specific integration into the Staphylococcus aureus chromosome. The staphylococcal cadmium-inducible P cad -cadC and constitutive P blaZ promoters were designed and analyzed in transcriptional fusions to the staphylococcal β-lactamase blaZ , the Vibrio fischeri luxAB , and the Aequorea victoria green fluorescent protein reporter genes. The modular design of the vector system provides great flexibility and variety. Questions about gene dosage, complementation, and cis-trans effects can now be conveniently addressed, so that this system constitutes an effective tool for studying gene regulation of staphylococci in various ecosystems.
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Cites
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Self-citationSynthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule.
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Self-citationProperties of a cryptic high-frequency transducing phage in Staphylococcus aureus
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Self-citation[27] Genetic systems in Staphylococci
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Deltcheva, E., Chylinski, K., Sharma, C. M., Gonzales, K., Chao, Y., Pirzada, Z. A., Eckert, M. R., Vogel, J., & Charpentier, E. (2011) · Nature · doi:10.1038/nature09886 · Cited by 2,563
Self-citationExploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials
Bikard, D., Euler, C. W., Jiang, W., Nussenzweig, P. M., Goldberg, G. W., Duportet, X., Fischetti, V. A., & Marraffini, L. A. (2014) · Nature Biotechnology · doi:10.1038/nbt.3043 · Cited by 986
A modular system for Clostridium shuttle plasmids
Heap, J. T., Pennington, O. J., Cartman, S. T., & Minton, N. P. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.05.004 · Cited by 489
Mashburn‐Warren, L., Morrison, D. A., & Federle, M. J. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07361.x · Cited by 287
Painter, K. L., Strange, E., Parkhill, J., Bamford, K. B., Armstrong-James, D., & Edwards, A. M. (2015) · Infection and Immunity · doi:10.1128/iai.03016-14 · Cited by 153
Metadata
- Authors
- Charpentier, E., Anton, A. I., Barry, P., Alfonso, B., Fang, Y., & Novick, R. P.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-10
- Volume
- 70
- Issue
- 10
- Pages
- 6076-6085
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2153453937
- Cited by
- 398
- References
- 25
- FWCI
- 2.69
- Type
- journal article
- Language
- en
- Open access
- green
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