Subtelomeric Silencing of the MTL3 Locus of Candida glabrata Requires yKu70, yKu80, and Rif1 Proteins
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Candida glabrata is a haploid opportunistic fungal pathogen that is phylogenetically related to Saccharomyces cerevisiae. Even though C. glabrata has no known sexual cycle, it contains, like S. cerevisiae, three mating type-like loci (MTL) called MTL1, MTL2, and MTL3, as well as most of the genes required for mating, meiosis, and sporulation. MTL1 is localized at an internal position on chromosome B and is thought to be the locus corresponding to the MAT locus in S. cerevisiae. MTL2 and MTL3 are localized close to two telomeres on different chromosomes (29.4 kb from Chr E-L and 10.5 kb from Chr B-L, respectively). By using URA3 reporter gene insertions at the three MTL loci, we found that in contrast to the case for S. cerevisiae, only MTL3 is subject to transcriptional silencing while MTL2 is transcriptionally active, and this is in agreement with previously reported data. We found that the silencing of MTL3 is nucleated primarily at the left telomere of chromosome B and spreads over 12 kb to MTL3, rather than nucleating at flanking, closely positioned cis-acting silencers, like those flanking HMR and HML of S. cerevisiae. Interestingly, the silencing of MTL3 absolutely requires the yKu70, yKu80, and Rif1 proteins, in sharp contrast to the silencing of the HM loci of S. cerevisiae. In addition, we found that several cell type-specific genes are expressed in C. glabrata regardless of the presence, or even absence, of mating type information at any of the MTL loci.
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CgMTL1 and CgMTL2 are transcriptionally active, while CgMTL3 is subjected to subtelomeric silencing.
“While CgMTL1 and CgMTL2 are transcriptionally active, CgMTL3 is subjected to subtelomeric silencing [77].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [77]
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- Authors
- Ramírez-Zavaleta, C. Y., Salas-Delgado, G. E., De Las Peñas, A., & Castaño, I.
- Affiliations
- Institute for Scientific and Technological Research (MX)
- Instituto Potosino de Investigación Científica y Tecnológica (IPICYT), Camino a la Presa San José 2055, Lomas 4a sección, San Luis Potosí SLP, Mexico
- Corresponding author
- Castaño, Irene
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2010-10
- Volume
- 9
- Issue
- 10
- Pages
- 1602-1611
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- PMC
- PMC2950432
- OpenAlex
- W2127446409
- Cited by
- 18
- References
- 58
- Citations by year
- 2025: 1 · 2024: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 3 · 2013: 1 · 2012: 3
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Eukaryotic Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Consejo Nacional de Ciencia y Tecnología
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- Candida glabrata, mating-type locus, transcriptional silencing, Ku70, Ku80, RIF1, telomeres, cell type-specific gene expression
- MeSH terms
- DNA-Binding Proteins, Fungal Proteins, Gene Expression Regulation, Fungal, Telomere, Gene Silencing, Telomere-Binding Proteins, Candida glabrata, Genes, Mating Type, Fungal
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