Involvement of the Saccharomyces cerevisiae Hydrolase Ldh1p in Lipid Homeostasis
Open accessAbstract
Here, we report the functional characterization of the newly identified lipid droplet hydrolase Ldh1p. Recombinant Ldh1p exhibits esterase and triacylglycerol lipase activities. Mutation of the serine in the hydrolase/lipase motif GXSXG completely abolished esterase activity. Ldh1p is required for the maintenance of a steady-state level of the nonpolar and polar lipids of lipid droplets. A characteristic feature of the Saccharomyces cerevisiae Δ ldh1 strain is the appearance of giant lipid droplets and an excessive accumulation of nonpolar lipids and phospholipids upon growth on medium containing oleic acid as a sole carbon source. Ldh1p is thought to play a role in maintaining the lipid homeostasis in yeast by regulating both phospholipid and nonpolar lipid levels.
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Lipid droplets play an important role in overcoming the potential toxic effect of abnormal sterols and free fatty acids, and affect cellular energy homeostasis and lipid metabolism.
“Lipid droplets not only play an important role in overcoming the potential toxic effect of abnormal sterols and free fatty acids (FA), but also affect cellular energy homeostasis and lipid metabolism.”
From The synthesis, regulation, and functions of sterols in<i>Candida albicans</i>: Well-known but still lots to learn (2016) · cites this paper as [50]
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Yeast lipid metabolism at a glance
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- Authors
- Debelyy, M. O., Thoms, S., Connerth, M., Daum, G., & Erdmann, R.
- Affiliations
- Ruhr University Bochum (DE)
- Graz University of Technology (AT)
- Corresponding author
- Erdmann, Ralf
- Venue
- Eukaryotic Cell
- Publisher
- American Society for Microbiology
- Published
- 2011-06
- Volume
- 10
- Issue
- 6
- Pages
- 776-781
- ISSN
- 1535-9778 (ISSN-L)
- 1535-9786
- PMC
- PMC3127674
- OpenAlex
- W2137345000
- Cited by
- 29
- References
- 42
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2020: 1 · 2019: 1 · 2018: 3 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 4
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Locations
- Eukaryotic Cell (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Lipids, and Metabolism
- Keywords
- lipid homeostasis, esterase activity, triacylglycerol lipase, phospholipid accumulation, non-polar lipids, oleic acid, Saccharomyces cerevisiae
- MeSH terms
- Amino Acid Sequence, Esterases, Membrane Proteins, Molecular Sequence Data, Phospholipases A, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Organelles, Sequence Alignment, Amino Acid Motifs, Protein Transport, Saccharomyces cerevisiae Proteins, Lipid Metabolism, Organelle Size, Gene Knockout Techniques, Enzyme Assays
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