Development of a Contemporary Animal Model of Candida albicans-Associated Denture Stomatitis Using a Novel Intraoral Denture System
Open accessAbstract
Denture stomatitis (DS) is a fungal infection characterized by inflammation of the oral mucosa in direct contact with the denture and affects up to 50% of denture wearers. Despite the prevalence, very little is known about the role of fungal or host factors that contribute to pathogenesis. Recently, we developed a novel intraoral denture system for rodent research. This denture system consists of custom-fitted fixed and removable parts to allow repeated sampling and longitudinal studies. The purpose of this study was to use this denture system to develop a clinically relevant animal model of DS. To establish DS, rats were inoculated with pelleted Candida albicans , which resulted in sustained colonization of the denture and palate for 8 weeks postinoculation. Biofilm formation on the denture was observed by week 4 and on the palate by week 6 postinoculation. Rats were monitored for clinical signs of disease by assigning a clinical score after macroscopic examination of the palate tissue according to Newton's method. By week 4 postinoculation, the majority of inoculated rats with dentures exhibited a clinical score of 1 (pinpoint erythema). By week 6 and week 8 postinoculation, increasing percentages of rats exhibited a clinical score of 2 (diffuse erythema/edema). Histological analysis of palate tissue demonstrated progressively increasing inflammatory cell recruitment throughout the time course of the infection. Palatal biofilm formation was commensurate with development of palatal erythema, which suggests a role for biofilm in the inflammatory response.
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Biofilm architectures in rat and rabbit central venous and indwelling urinary catheter models and in rat denture stomatitis models include numerous yeast cells in the basal region, and hyphae and extracellular matrix extending throughout the biofilm.
“Biofilm architectures in rat and rabbit central venous and indwelling urinary catheter models and in rat denture stomatitis models also include numerous yeast cells in the basal region, and hyphae and extracellular matrix extending throughout the biofilm (5, 84, 131, 134, 166, 173, 199).”
From <i>Candida albicans</i> Biofilms and Human Disease (2015) · cites this paper as [84]
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<i>Candida albicans</i> Biofilms and Human Disease
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- Authors
- Johnson, C. C., Yu, A., Lee, H., Fidel, P. L., & Noverr, M. C.
- Affiliations
- Louisiana State University (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2012-05
- Volume
- 80
- Issue
- 5
- Pages
- 1736-1743
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC3347462
- OpenAlex
- W2108324097
- Cited by
- 73
- References
- 53
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 7 · 2019: 2 · 2018: 2 · 2017: 12 · 2016: 9 · 2015: 7 · 2014: 10 · 2013: 4 · 2012: 1
- FWCI
- 4.86
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01de022069-05)
- National Institute of Dental and Craniofacial Research (R01-DE022069, R56DE022069)
- National Center for Research Resources (P20RR020160, P20RR20160-S1)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Dentistry › Periodontics › Oral Health Pathology and Treatment
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- denture stomatitis, Candida albicans, animal models, fungal biofilms, stomatitis, rat model, inflammatory cell recruitment, fungal infection
- MeSH terms
- Animals, Candida albicans, Candidiasis, Dentures, Disease Models, Animal, Humans, Male, Palate, Saliva, Stomatitis, Denture, Rats, Wistar, Biofilms, Rats
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