Fibrinogen-mediated adherence of group A Streptococcus to influenza A virus-infected cell cultures
Open accessAbstract
A quantitative radioassay was used to study the adherence of group A Streptococcus to Madin-Darby canine kidney cells infected with influenza A virus (strains FM1, Jap 305, and NWS) and reacted with fibrinogen. Treatment of virus-infected cell cultures with human fibrinogen significantly enhanced streptococcal adherence (P less than 0.0005) compared with adherence to untreated, virus-infected cells and uninfected control cells. Enhanced adherence was not seen with NWS virus-infected cell cultures or with virus-infected cells treated with human fibronectin, canine fibrinogen, or porcine fibrinogen. Human fibrinogen was shown to bind directly to surface membranes of virus-infected cells. Virus-infected cell cultures were incubated in the presence of tunicamycin, an antibiotic that inhibits glycosylation of virus-specific surface membrane glycoproteins. We found that with increasing antibiotic concentration there was a progressive decrease in fibrinogen-mediated streptococcal adherence. Adherence of 3H-labeled streptococci to fibrinogen-treated, virus-infected cell cultures showed saturation kinetics and could be blocked with monospecific antibodies against fibrinogen. These results suggest that human fibrinogen binds to a glycoprotein moiety on the surface of influenza A virus-infected cells, and that once bound the fibrinogen molecule acts as an "acquired" receptor for the attachment of group A Streptococcus. We postulate that this mechanism, it if occurs in vivo, might help explain the observed association between influenza A virus infection and subsequent bacterial superinfection with group A Streptococcus.
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- Authors
- Sanford, B. A., Davison, V. E., & Ramsay, M. A.
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1982-11
- Volume
- 38
- Issue
- 2
- Pages
- 513-520
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC347768
- OpenAlex
- W2099673971
- Cited by
- 33
- References
- 21
- Citations by year
- 2022: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2014: 2 · 2012: 2
- FWCI
- 2.83
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai017242-10)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Streptococcal Infections and Treatments
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- Streptococcus pyogenes, influenza A virus, fibrinogen, bacterial adhesion, viral infection, secondary bacterial infection, tunicamycin, glycosylation, MDCK cells, fibronectin
- MeSH terms
- Adhesiveness, Animals, Cell Line, Cell Membrane, Dogs, Fibrinogen, Fibronectins, Humans, Kidney, Influenza A virus, Species Specificity, Streptococcus pyogenes, Swine, Tunicamycin
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