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Cited by 134Pneumonia and Respiratory InfectionsRespiratory viral infections researchMicrobial infections and disease research

Adenovirus infection enhances in vitro adherence of Streptococcus pneumoniae.

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Håkansson, A, Kidd, A, Wadell, G, Sabharwal, H, Svanborg, C·1994·Infection and Immunity
DOI 10.1128/iai.62.7.2707-2714.1994PMC PMC302872
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Abstract

Viruses are thought to facilitate bacterial infections of the respiratory tract, but the mechanisms are poorly understood. The present study analyzed the effect of adenovirus on bacterial adherence to human respiratory tract epithelial cells. The human lung carcinoma cell line A549 was infected with adenovirus of types 1, 2, 3, 4, 5, and 9. At a multiplicity of infection of 75 particles per cell, cytopathic effects occurred in 75 to 100% of the cells within 48 h. The virus-infected cells were harvested at various times after infection and analyzed for the ability to bind strains of Haemophilus influenzae and Streptococcus pneumoniae. Adenovirus (types 1, 2, 3, and 5) commonly causing respiratory tract infections increased the binding of adherent S. pneumoniae strains to the cells. This effect was not seen for other adenovirus types. Adenovirus infection did not change the adherence of cells of poorly adhering strains of S. pneumoniae or H. influenzae. The increase in adherence of S. pneumoniae could be inhibited by the DNA synthesis inhibitor cytosine arabinofuranoside, which is known to block the late phase of the adenovirus infection. When electron microscopy was used, there was no evidence that virus particles bound directly to bacteria. Adherence was not affected by pretreatment of the cells with virus particles or viral proteins. This suggested that adenovirus infection upregulated receptors for S. pneumoniae. The increased attachment may be one mechanism by which viruses precondition the respiratory mucosa for bacterial infection.

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Crossref · 10.1128/iai.62.7.2707-2714.1994

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Authors
Håkansson, A., Kidd, A., Wadell, G., Sabharwal, H., & Svanborg, C.
Affiliations
Lund University (SE)
ORCID
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1994-07
Volume
62
Issue
7
Pages
2707-2714
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.62.7.2707-2714.1994
PMC
PMC302872
OpenAlex
W2042417907
Cited by
134
References
35
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 5 · 2022: 4 · 2021: 1 · 2020: 4 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 6 · 2014: 13 · 2013: 5 · 2012: 4
FWCI
6.52
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
  • Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
adenovirus, Streptococcus pneumoniae, bacterial adhesion, Haemophilus influenzae, respiratory infections, viral-bacterial interactions, airway epithelial cells, A549 cells, cytosine arabinofuranoside, cytopathic effect
MeSH terms
Adenoviridae, Adenoviridae Infections, Bacterial Adhesion, Capsid, Cells, Cultured, Cytarabine, Epithelium, Humans, Nasopharynx, Pneumococcal Infections, Streptococcus pneumoniae, Virus Replication, Capsid Proteins

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