Only Viable Parasites Are Detected by PCR following Clearance of Rodent Malarial Infections by Drug Treatment or Immune Responses
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Detection and analysis of pathogens by PCR plays an important role in infectious disease research. The value of these studies would be diminished if nuclear material from dead parasites were found to remain in circulation for extended periods and thus result in positive amplification. This possibility was tested in experimental rodent malaria infections. Blood samples were obtained from infected mice during and following drug or immune clearance of Plasmodium chabaudi chabaudi parasitemias. Detection of parasite DNA by a sensitive Plasmodium-specific PCR amplification assay was associated with the presence of viable parasites, as detected by subinoculation. No parasite DNA could be detected by PCR 48 h after the injection of killed parasites into mice. Nuclear material from parasites removed by drug or immune responses is rapidly cleared from the circulation and does not contribute significantly to amplification. Thus, results from PCR analysis of malaria-infected blood accurately reflect the presence of live parasites.
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P. falciparum parasitaemia below the threshold of microscopy but detected by PCR are likely to represent viable parasites at very low densities.
“P. falciparum-parasitaemia below the threshold of microscopy but detected by PCR are likely to represent viable parasites at very low densities [32]”
From Intermittent preventive treatment with Sulphadoxine-Pyrimethamine (IPTp-SP) is associated with protection against sub-microscopic P. falciparum infection in pregnant women during the low transmission dry season in southwestern Cameroon: A Semi - longitudinal study (2022) · cites this paper as [32]
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- Authors
- Jarra, W., & Snounou, G.
- Affiliations
- National Institute for Medical Research (TZ)
- Northwick Park Hospital (GB)
- ORCID
- Snounou, Georges
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1998-08
- Volume
- 66
- Issue
- 8
- Pages
- 3783-3787
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC108417
- OpenAlex
- W2144048008
- Cited by
- 71
- References
- 37
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 5 · 2016: 4 · 2015: 2 · 2014: 3 · 2013: 5 · 2012: 2
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- rodent malaria, Plasmodium chabaudi chabaudi, parasite DNA detection, parasite viability, drug clearance, immune clearance, subinoculation
- MeSH terms
- Animals, Disease Models, Animal, Freezing, Malaria, Male, Mice, Inbred CBA, Sensitivity and Specificity, DNA, Protozoan, Polymerase Chain Reaction, Plasmodium chabaudi, Mice
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