The StcE Protease Contributes to Intimate Adherence of Enterohemorrhagic Escherichia coli O157:H7 to Host Cells

Grys, Thomas E.; Siegel, Matthew B.; Lathem, Wyndham W.; Welch, Rodney A.

doi:10.1128/iai.73.3.1295-1303.2005

Cited by 138Escherichia coli research studiesBacterial Genetics and BiotechnologyClostridium difficile and Clostridium perfringens research

The StcE Protease Contributes to Intimate Adherence of Enterohemorrhagic Escherichia coli O157:H7 to Host Cells

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Grys, Thomas E., Siegel, Matthew B., Lathem, Wyndham W., Welch, Rodney A.·2005·Infection and Immunity

DOI 10.1128/iai.73.3.1295-1303.2005PMC PMC1064933

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Abstract

Enterohemorrhagic Escherichia coli(EHEC) O157:H7 is a diarrheal pathogen that causes attaching and effacing (A/E) lesions on intestinal epithelial cells. Strains of the O157 serogroup carry the large virulence plasmid pO157, which encodes the etp type II secretion system that secretes the genetically linked zinc metalloprotease StcE. The Ler regulator controls expression of many genes involved in A/E lesion formation, as well as StcE, suggesting StcE may be important at a similar time during colonization. Our laboratory has previously demonstrated that StcE cleaves C1-esterase inhibitor, a regulator of multiple inflammation pathways. Here we report two new substrates for StcE, mucin 7 and glycoprotein 340, and that purified StcE reduces the viscosity of human saliva. We tested the hypothesis that StcE contributes to intimate adherence of EHEC to host cells by cleavage of glycoproteins from the cell surface. The fluorescent actin stain (FAS) test was used to observe the intimate adherence represented by fluorescently stained bacteria colocalized with regions of bundled actin formed on HEp-2 cells. An E. coli O157:H7 strain with a stcE gene deletion was not affected in its ability to generally adhere to HEp-2 cells, but it did score threefold lower on the FAS test than wild-type or complemented strains. Addition of exogenous recombinant StcE increased intimate adherence of the mutant to wild-type levels. Thus, StcE may help block host clearance of E. coli O157:H7 by destruction of some classes of glycoproteins, and it contributes to intimate adherence of E. coli O157:H7 to the HEp-2 cell surface.

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StcE and SslE, metalloproteases from diarrheagenic E. coli strains, cleave mucin glycoproteins, which may help the pathogen to reach the epithelium.
“StcE and SslE, metalloproteases from diarrheagenic E. coli strains, also cleave mucin glycoproteins, which may help the pathogen to reach the epithelium.74–76”
From The emerging roles of bacterial proteases in intestinal diseases (2023) · cites this paper as [75]
The zinc metalloproteases StcE and SslE secreted by E. coli EHEC and EPEC/ETEC respectively contribute to intimate adherence of these bacteria to host cells, a process essential for colonization.
“Finally, the zinc metalloproteases StcE and SslE secreted by E. coli EHEC and EPEC/ETEC respectively, contribute to intimate adherence of these bacteria to host cells, a process that is essential for colonization.73–78”
From The emerging roles of bacterial proteases in intestinal diseases (2023) · cites this paper as [75]

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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902 · see citing works
Diarrheagenic Escherichia coli
Nataro, J. P., & Kaper, J. B. (1998) · Clinical Microbiology Reviews · doi:10.1128/cmr.11.1.142 · Cited by 5,109 · see citing works
Transduction of linked genetic characters of the host by bacteriophage P1
Lennox, E. S. (1955) · Virology · doi:10.1016/0042-6822(55)90016-7 · Cited by 2,604 · see citing works
Genome sequence of enterohaemorrhagic Escherichia coli O157:H7
Perna, N. T., Plunkett, G., Burland, V., Mau, B., Glasner, J. D., Rose, D. J., Mayhew, G. F., Evans, P. S., Gregor, J., Kirkpatrick, H. A., et al. (2001) · Nature · doi:10.1038/35054089 · Cited by 2,035 · see citing works
Self-citation
Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells.
Kenny, B., Devinney, R., Stein, M., Reinscheid, D., Frey, E., & Finlay, B. (1997) · Cell · doi:10.1016/s0092-8674(00)80437-7 · Cited by 1,258 · see citing works
A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens.
McDaniel, T. K., Jarvis, K. G., Donnenberg, M. S., & Kaper, J. B. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.5.1664 · Cited by 1,247 · see citing works
The indigenous gastrointestinal microflora
Berg, R. (1996) · Trends in Microbiology · doi:10.1016/0966-842x(96)10057-3 · Cited by 1,153 · see citing works
A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.
Jerse, A. E., Yu, J., Tall, B. D., & Kaper, J. B. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.20.7839 · Cited by 1,125 · see citing works
Mucin-Type Glycoproteins
Strous, G. J., & Dekker, J. P. (1992) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239209082559 · Cited by 927 · see citing works
Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli
Knutton, S., Baldwin, T. J., WILLIAMS, P. H., & McNeish, A. S. (1989) · Infection and Immunity · doi:10.1128/iai.57.4.1290-1298.1989 · Cited by 875 · see citing works
Enteropathogenic and enterohaemorrhagic Escherichia coli : more subversive elements
Frankel, G., Phillips, A. D., Rosenshine, I., Dougan, G., Kaper, J., & Knutton, S. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01144.x · Cited by 747 · see citing works
Typical and Atypical EnteropathogenicEscherichia coli
Trabulsi, L. R., Keller, R., & Gomes, T. A. T. (2002) · Emerging infectious diseases · doi:10.3201/eid0805.010385 · Cited by 693 · see citing works
An Inducible Bundle-Forming Pilus of Enteropathogenic Escherichia coli
Girón, J. A., Ho, A. S.-Y., & Schoolnik, G. K. (1991) · Science · doi:10.1126/science.1683004 · Cited by 604 · see citing works
A novel EspA‐associated surface organelle of enteropathogenic Escherichia coli involved in protein translocation into epithelial cells
Knutton, S., Rosenshine, I., Pallen, M. J., Nisan, I., Neves, B. C., Bain, C., Wolff, C., Gordon Dougan, B., & Frankel, G. M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.8.2166 · Cited by 578 · see citing works
Probiotics inhibit enteropathogenic E. coliadherence in vitro by inducing intestinal mucin gene expression.
Mack, D., Michail, S., Wei, S., McDougall, L., & Hollingsworth, M. (1999) · American Journal of Physiology - Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.1999.276.4.g941 · Cited by 544 · see citing works
Thickness of adherent mucus gel on colonic mucosa in humans and its relevance to colitis.
Pullan, R. D., Thomas, G. A. O., Rhodes, M. A., Newcombe, R. G., Williams, G. T., Allen, A., & Rhodes, J. M. (1994) · Gut · doi:10.1136/gut.35.3.353 · Cited by 467 · see citing works
Type IV Pili, Transient Bacterial Aggregates, and Virulence of Enteropathogenic Escherichia coli
Bieber, D., Ramer, S. W., Wu, C.-Y., Murray, W. J., Tobe, T., Fernandez, R., & Schoolnik, G. K. (1998) · Science · doi:10.1126/science.280.5372.2114 · Cited by 465 · see citing works
Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7).
Bobek, L. A., Tsai, H., Biesbrock, A. R., & Levine, M. J. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80762-5 · Cited by 458 · see citing works
The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional activator, the locus of enterocyte effacement (LEE)‐encoded regulator (Ler)
Mellies, J. L., Elliott, S. J., Sperandio, V., Donnenberg, M. S., & Kaper, J. B. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01473.x · Cited by 390 · see citing works
Structural organization and classification of the human mucin genes
Moniaux, N. (2001) · Frontiers in bioscience · doi:10.2741/moniaux · Cited by 387 · see citing works
The role of the eae gene of enterohemorrhagic Escherichia coli in intimate attachment in vitro and in a porcine model.
Donnenberg, M. S., Tzipori, S. R., McKee, M. L., O’Brien, A. D., Alroy, J., & Kaper, J. B. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116718 · Cited by 363 · see citing works
Characterization of Pic, a Secreted Protease of Shigella flexneri and Enteroaggregative Escherichia coli
Henderson, I. R., Czeczulin, J., Eslava, C., Noriega, F., & Nataro, J. P. (1999) · Infection and Immunity · doi:10.1128/iai.67.11.5587-5596.1999 · Cited by 357 · see citing works
The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7
Burland, V. D. (1998) · Nucleic Acids Research · doi:10.1093/nar/26.18.4196 · Cited by 353 · see citing works
Acid tolerance of enterohemorrhagic Escherichia coli
Benjamin, M. M., & Datta, A. R. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.4.1669-1672.1995 · Cited by 346 · see citing works
Mucins in the gastrointestinal tract in health and disease
Anthony, P. P. (2001) · Frontiers in bioscience · doi:10.2741/corfield · Cited by 343 · see citing works
Human C.hivin.1 inhibitor: primary structure, cDNA cloning, and chromosomal localization
Bock, S. C., Skriver, K., Nielsen, E., Thoegersen, H. C., Wiman, B., Donaldson, V. H., Eddy, R. L., Marrinan, J. A., & Radziejewska, E. (1986) · Biochemistry · doi:10.1021/bi00363a018 · Cited by 330 · see citing works
C1-Esterase Inhibitor: An Anti-Inflammatory Agent and Its Potential Use in the Treatment of Diseases Other Than Hereditary Angioedema
Caliezi, C., Wuillemin, W. A., Zeerleder, S. S., Redondo, M., Eisele, B., & Hack, C. E. (2000) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01437-6 · Cited by 311 · see citing works
The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid
Jerse, A. E., & Kaper, J. B. (1991) · Infection and Immunity · doi:10.1128/iai.59.12.4302-4309.1991 · Cited by 307 · see citing works
Starvation- and stationary-phase-induced acid tolerance in Escherichia coli O157:H7
Arnold, K. W., & Kaspar, C. W. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.5.2037-2039.1995 · Cited by 276 · see citing works
KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7
Brunder, W., Schmidt, H., & Karch, H. (1996) · Microbiology · doi:10.1099/13500872-142-11-3305 · Cited by 259 · see citing works
Enterohemorrhagic Escherichia coli O157:H7 Produces Tir, Which Is Translocated to the Host Cell Membrane but Is Not Tyrosine Phosphorylated
DeVinney, R., Stein, M., Reinscheid, D. J., Abe, A., Ruschkowski, S., & Finlay, B. B. (1999) · Infection and Immunity · doi:10.1128/iai.67.5.2389-2398.1999 · Cited by 244 · see citing works
Enteropathogenic Escherichia coli (EPEC) adhesion to intestinal epithelial cells: role of bundle-forming pili (BFP), EspA filaments and intimin
Cleary, J., Lai, L., Shaw, R. K., Straatman-Iwanowska, A. A., Donnenberg, M. S., Frankel, G. M., & Knutton, S. (2004) · Microbiology · doi:10.1099/mic.0.26740-0 · Cited by 235 · see citing works
Salivary Agglutinin, Which Binds Streptococcus mutansand Helicobacter pylori, Is the Lung Scavenger Receptor Cysteine-rich Protein gp-340
Prakobphol, A., Xu, F., Hoang, V. M., Larsson, T. P., Bergström, J., Johansson, I., Frängsmyr, L., Holmskov, U., Leffler, H., Nilsson, C., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m006928200 · Cited by 219 · see citing works
Isolation and Characterization of a New Member of the Scavenger Receptor Superfamily, Glycoprotein-340 (gp-340), as a Lung Surfactant Protein-D Binding Molecule
Holmskov, U., Lawson, P., Teisner, B., Tornøe, I., Willis, A. C., Morgan, C., Koch, C., & Reid, K. B. M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.21.13743 · Cited by 214 · see citing works
Cloning of gp-340, a putative opsonin receptor for lung surfactant protein D
Holmskov, U., Mollenhauer, J., Madsen, J., Vitved, L., Grønlund, J., Tornøe, I., Kliem, A., Reid, K. B. M., Poustka, A., & Skjødt, K. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.19.10794 · Cited by 205 · see citing works
A plasmid of enterohemorrhagic Escherichia coli O157:H7 is required for expression of a new fimbrial antigen and for adhesion to epithelial cells
Karch, H., Heesemann, J., Laufs, R., O’Brien, A. D., Tacket, C. O., & Levine, M. M. (1987) · Infection and Immunity · doi:10.1128/iai.55.2.455-461.1987 · Cited by 201 · see citing works
Enterohemorrhagic Escherichia coli O157:H7 requires intimin to colonize the gnotobiotic pig intestine and to adhere to HEp-2 cells
McKee, M. L., Melton‐Celsa, A. R., Moxley, R. A., Francis, D. H., & O’Brien, A. D. (1995) · Infection and Immunity · doi:10.1128/iai.63.9.3739-3744.1995 · Cited by 198 · see citing works
Vibrio cholerae hemagglutinin/protease, colonial variation, virulence, and detachment
Finkelstein, R. A., Boesman-Finkelstein, M., Chang, Y., & Häse, C. C. (1992) · Infection and Immunity · doi:10.1128/iai.60.2.472-478.1992 · Cited by 197 · see citing works
StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor
Lathem, W. W., Grys, T. E., Witowski, S. E., Torres, A. G., Kaper, J. B., Tarr, P. I., & Welch, R. A. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02997.x · Cited by 183 · see citing works
Self-citation
DMBT1 encodes a protein involved in the immune defense and in epithelial differentiation and is highly unstable in cancer.
Mollenhauer, J., Herbertz, S., Holmskov, U., Tolnay, M., Krebs, I., Merlo, A. U., Schrøder, H. D., Maier, D., Breitling, F., Wiemann, S., et al. (2000) · PubMed · Cited by 179 · see citing works
Vibrio cholerae hemagglutinin/lectin/protease hydrolyzes fibronectin and ovomucin: F. M. Burnet revisited
Finkelstein, R. A., Boesman-Finkelstein, M., & Holt, P. S. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.4.1092 · Cited by 176 · see citing works
Tails of two Tirs: actin pedestal formation by enteropathogenic E. coli and enterohemorrhagic E. coli O157:H7
Campellone, K. G. (2003) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(03)00005-5 · Cited by 161 · see citing works
Identification of the Bacteria-binding Peptide Domain on Salivary Agglutinin (gp-340/DMBT1), a Member of the Scavenger Receptor Cysteine-rich Superfamily
Bikker, F. J., Ligtenberg, A. J. M., Nazmi, K., Veerman, E. C. I., Hof, W. V. ‘., Bolscher, J. G. M., Poustka, A., Amerongen, A. V. N., & Mollenhauer, J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m203788200 · Cited by 157 · see citing works
Cloning and nucleotide sequence of the Vibrio cholerae hemagglutinin/protease (HA/protease) gene and construction of an HA/protease-negative strain
Häse, C. C., & Finkelstein, R. A. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.11.3311-3317.1991 · Cited by 155 · see citing works
Purification of a low-molecular-weight, mucin-type glycoprotein from human submandibular-sublingual saliva
Prakobphol, A., LEVINE, M. J., Tabak, L. A., & Reddy, M. S. (1982) · Carbohydrate Research · doi:10.1016/s0008-6215(00)81896-0 · Cited by 128 · see citing works
Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7
Fitzhenry, R., Pickard, D., Hartland, E. L., Reece, S., Gordon Dougan, B., Phillips, A. D., & Frankel, G. M. (2002) · Gut · doi:10.1136/gut.50.2.180 · Cited by 126 · see citing works
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes
Chen, T. R. (2008) · Cytogenetics and Cell Genetics · doi:10.1159/000132579 · Cited by 116 · see citing works
Two Enteropathogenic Escherichia coli Type III Secreted Proteins, EspA and EspB, Are Virulence Factors
Abe, A., Heczko, U., Hegele, R. G., & Finlay, B. B. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.10.1907 · Cited by 114 · see citing works
Glycoprotein-340 Binds Surfactant Protein-A (SP-A) and Stimulates Alveolar Macrophage Migration in an SP-A–Independent Manner
Tino, M. J., & Wright, J. R. (1999) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.20.4.3439 · Cited by 105 · see citing works
Structure, Function and Gene Expression of Epithelial Mucins
Seregni, E. C., Botti, C., Massaron, S., Lombardo, C., Capobianco, A. M. L., Bogni, A., & Bombardieri, E. (1997) · Tumori Journal · doi:10.1177/030089169708300301 · Cited by 103 · see citing works
Atypical EnteropathogenicEscherichia coliStrains: Phenotypic and Genetic Profiling Reveals a Strong Association between EnteroaggregativeE. coliHeat‐Stable Enterotoxin and Diarrhea
Dulguer, M. V., Fabbricotti, S. H., Bando, S. Y., Moreira‐Filho, C. A., Fagundes‐Neto, U., & Scaletsky, I. C. A. (2003) · The Journal of Infectious Diseases · doi:10.1086/379666 · Cited by 101 · see citing works
DMBT1, a regulator of mucosal homeostasis through the linking of mucosal defense and regeneration?
Kang, W., & Reid, K. B. M. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00217-5 · Cited by 97 · see citing works
High prevalence of atypical enteropathogenic Escherichia coli (EPEC) in Norwegian children with diarrhoea
Afset, J. E., Bergh, K., & Bevanger, L. S. (2003) · Journal of Medical Microbiology · doi:10.1099/jmm.0.05287-0 · Cited by 91 · see citing works
The genomic structure of the DMBT1 gene: evidence for a region with susceptibility to genomic instability
Mollenhauer, J., Holmskov, U., Wiemann, S., Krebs, I., Herbertz, S., Madsen, J., Kioschis, P., Coy, J. F., & Poustka, A. (1999) · Oncogene · doi:10.1038/sj.onc.1203071 · Cited by 90 · see citing works
Deleted in Malignant Brain Tumors 1 is a versatile mucin-like molecule likely to play a differential role in digestive tract cancer.
Mollenhauer, J., Herbertz, S., Helmke, B. M., Kollender, G., Krebs, I., Madsen, J., Holmskov, U., Sorger, K., Schmitt, L., Wiemann, S., et al. (2001) · PubMed · Cited by 90 · see citing works
Environmental Signals Controlling Production of Hemagglutinin/Protease in Vibrio cholerae
Benítez, J. A., Silva, A. J., & Finkelstein, R. A. (2001) · Infection and Immunity · doi:10.1128/iai.69.10.6549-6553.2001 · Cited by 86 · see citing works
Virulence properties of atypical EPEC strains
Pélayo, J. S., Scaletsky, I. C. A., Pedroso, M. Z., Sperandio, V., Girón, J. A., Frankel, G. M., & Trabulsi, L. R. (1999) · Journal of Medical Microbiology · doi:10.1099/00222615-48-1-41 · Cited by 83 · see citing works
Influence of the 60-megadalton plasmid on adherence of Escherichia coli O157:H7 and genetic derivatives
Toth, I., Cohen, M. L., Rumschlag, H. S., Riley, L. W., WHITE, E. H., Carr, J., Bond, W. W., & Wachsmuth, I. K. (1990) · Infection and Immunity · doi:10.1128/iai.58.5.1223-1231.1990 · Cited by 82 · see citing works
The recombinant N-terminal region of human salivary mucin MG2 (MUC7) contains a binding domain for oral Streptococci and exhibits candidacidal activity
Liu, B., Rayment, S. A., Gyurko, C., Oppenheim, F. G., Offner, G. D., & Troxler, R. F. (2000) · Biochemical Journal · doi:10.1042/bj3450557 · Cited by 74 · see citing works
A monoclonal antibody directed against high Mr salivary mucins recognizes the SO3-3Galβ1-3GlcNAc moiety of sulfo-Lewisa: a histochemical survey of human and rat tissue
Veerman, E. C. I., Bolscher, J. G. M., Appelmelk, B. J., Bloemena, E., van den Berg, T. K., & Amerongen, A. V. N. (1997) · Glycobiology · doi:10.1093/glycob/7.1.37 · Cited by 71 · see citing works
Potentiation of C1 Esterase Inhibitor by StcE, a Metalloprotease Secreted by Escherichia coli O157:H7
Lathem, W. W., Bergsbaken, T., & Welch, R. A. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20030255 · Cited by 68 · see citing works
Self-citation
Adherence of Vibrio cholerae to cultured differentiated human intestinal cells: an in vitro colonization model
Benítez, J. A., Spelbrink, R. G., Silva, A. J., Phillips, T. E., Stanley, C. M., Boesman-Finkelstein, M., & Finkelstein, R. A. (1997) · Infection and Immunity · doi:10.1128/iai.65.8.3474-3477.1997 · Cited by 68 · see citing works
[26] Methods for studying adhesion of diarrheagenic Escherichia coli
Donnenberg, M. S., & Nataro, J. P. (1995) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(95)53028-2 · Cited by 62 · see citing works
Human Low-Molecular-Weight Salivary Mucin Expresses the Sialyl Lewisx Determinant and Has L-Selectin Ligand Activity
Prakobphol, A., Thomsson, K. A., Hansson, G. C., Rosen, S. D., Singer, M. S., Phillips, N. J., Medzihradszky, K. F., Burlingame, A. L., Leffler, H., & Fisher, S. J. (1998) · Biochemistry · doi:10.1021/bi972612a · Cited by 56 · see citing works
HeLa-Like Marker Chromosomes and Type-A Variant Glucose-6-phosphate Dehydrogenase Isoenzyme in Human Cell Cultures Producing Mason-Pfizer Monkey Virus-Like Particles2
Nelson‐Rees, W. A., Zhdanov, V. M., Hawthorne, P. K., & Flandermeyer, R. R. (1974) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/53.3.751 · Cited by 56 · see citing works
Functional analysis of the serpin domain of C1 inhibitor.
Coutinho, M., Aulak, K. S., & Davis, A. E. (1994) · The Journal of Immunology · doi:10.4049/jimmunol.153.8.3648 · Cited by 52 · see citing works
Separate Oligosaccharide Determinants Mediate Interactions of the Low-Molecular-Weight Salivary Mucin with Neutrophils and Bacteria
Prakobphol, A., Tangemann, K., Rosen, S. D., Hoover, C. I., Leffler, H., & Fisher, S. J. (1999) · Biochemistry · doi:10.1021/bi990145m · Cited by 52 · see citing works
Role for Phosphoglucomutase in Vibrio fischeri-Euprymna scolopes Symbiosis
DeLoney, C. R., Bartley, T. M., & Visick, K. L. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.18.5121-5129.2002 · Cited by 48 · see citing works
Studies on Escherichia coli serotype O157:H7 strains containing a 60-MDa plasmid and on 60-MDa plasmid-cured derivatives
Fratamico, P. M., Bhaduri, S., & Buchanan, R. L. (1993) · Journal of Medical Microbiology · doi:10.1099/00222615-39-5-371 · Cited by 44 · see citing works
Characteristics of HeLa strains: Permanent vs. variable features
Nelson‐Rees, W. A., Hunter, L. M., Darlington, G., & O’Brien, S. J. (2008) · Cytogenetics and Cell Genetics · doi:10.1159/000131490 · Cited by 41 · see citing works
Polymorphisms within EspA Filaments of Enteropathogenic and Enterohemorrhagic Escherichia coli
Neves, B. C., Shaw, R. K., Frankel, G. M., & Knutton, S. (2003) · Infection and Immunity · doi:10.1128/iai.71.4.2262-2265.2003 · Cited by 34 · see citing works
Acquisition ofstcE,a C1 Esterase Inhibitor–Specific Metalloprotease, during the Evolution ofEscherichia coliO157:H7
Lathem, W. W., Bergsbaken, T., Witowski, S. E., Perna, N. T., & Welch, R. A. (2003) · The Journal of Infectious Diseases · doi:10.1086/374719 · Cited by 22 · see citing works
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Recent Advances in Understanding Enteric Pathogenic Escherichia coli
Croxen, M. A., Law, R. J., Scholz, R., Keeney, K. M., Wlodarska, M., & Finlay, B. B. (2013) · Clinical Microbiology Reviews · doi:10.1128/cmr.00022-13 · Cited by 1,578 · see citing works
Shiga toxin-producing Escherichia coli: An overview1
Gyles, C. L. (2007) · Journal of Animal Science · doi:10.2527/jas.2006-508 · Cited by 850 · see citing works
Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier
Martens, E. C., Neumann, M., & Desai, M. S. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0036-x · Cited by 711 · see citing works
Role of the gut microbiota in type 2 diabetes and related diseases
Yang, G., Wei, J., Liu, P., Zhang, Q., Tian, Y., Hou, G., Meng, L., Xin, Y., & Jiang, X. (2021) · Metabolism · doi:10.1016/j.metabol.2021.154712 · Cited by 418 · see citing works
Type II secretion: a protein secretion system for all seasons
Cianciotto, N. P. (2005) · Trends in Microbiology · doi:10.1016/j.tim.2005.09.005 · Cited by 373 · see citing works
Bacteria and host interactions in the gut epithelial barrier
Ashida, H., Ogawa, M., Kim, M., Mimuro, H., & Sasakawa, C. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.741 · Cited by 335 · see citing works
The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins
Malaker, S. A., Pedram, K., Ferracane, M. J., Bensing, B. A., Krishnan, V., Pett, C., Yu, J., Woods, E. C., Kramer, J. R., Westerlind, U., et al. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1813020116 · Cited by 262 · see citing works
Enterohemorrhagic Escherichia coli Reduces Mucus and Intermicrovillar Bridges in Human Stem Cell-Derived Colonoids
In, J., Foulke-Abel, J., Zachos, N. C., Hansen, A.-M., Kaper, J. B., Bernstein, H. D., Halushka, M., Blutt, S., Estes, M. K., Donowitz, M., et al. (2016) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2015.10.001 · Cited by 239 · see citing works
Enteropathogenic and Enterohemorrhagic Escherichia coli Virulence Gene Regulation
Mellies, J. L., Barron, A., & Groat-Carmona, A. M. (2007) · Infection and Immunity · doi:10.1128/iai.01927-06 · Cited by 210 · see citing works
Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond
Cianciotto, N. P., & White, R. C. (2017) · Infection and Immunity · doi:10.1128/iai.00014-17 · Cited by 208 · see citing works
Molecular and Cellular Mechanisms of Ectodomain Shedding
Hayashida, K., Bartlett, A. H., Chen, Y., & Park, P. W. (2010) · The Anatomical Record · doi:10.1002/ar.20757 · Cited by 198 · see citing works
Experimental models to study intestinal microbes–mucus interactions in health and disease
Etienne‐Mesmin, L., Chassaing, B., Desvaux, M., Paepe, K. D., Gresse, R., Sauvaitre, T., Forano, É., de Wiele, T. V., Schüller, S., Juge, N., et al. (2019) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuz013 · Cited by 179 · see citing works
Pathogenesis of Shiga-Toxin Producing Escherichia coli
Melton‐Celsa, A. R., Mohawk, K. L., Teel, L. D., & O’Brien, A. D. (2011) · Current topics in microbiology and immunology · doi:10.1007/82_2011_176 · Cited by 177 · see citing works
Comparative Genomics Reveals What Makes An Enterobacterial Plant Pathogen
Toth, I. K., Pritchard, L., & Birch, P. R. J. (2006) · Annual Review of Phytopathology · doi:10.1146/annurev.phyto.44.070505.143444 · Cited by 163 · see citing works
The Pic Protease of Enteroaggregative Escherichia coli Promotes Intestinal Colonization and Growth in the Presence of Mucin
Harrington, S. M., Sheikh, J., Henderson, I. R., Ruiz-Perez, F., Cohen, P. S., & Nataro, J. P. (2009) · Infection and Immunity · doi:10.1128/iai.01494-08 · Cited by 156 · see citing works
An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium
Mahajan, A., Currie, C., Mackie, S., Tree, J. J., McAteer, S. P., McKendrick, I. J., McNeilly, T. N., Roe, A. J., Ragione, R. M. L., Woodward, M. J., et al. (2008) · Cellular Microbiology · doi:10.1111/j.1462-5822.2008.01244.x · Cited by 151 · see citing works
Role of Escherichia coli O157:H7 Virulence Factors in Colonization at the Bovine Terminal Rectal Mucosa
Sheng, H., Lim, J. Y., Knecht, H. J., Li, J., & Hovde, C. J. (2006) · Infection and Immunity · doi:10.1128/iai.00406-06 · Cited by 144 · see citing works
Species-specific enhancement of enterohemorrhagic E. coli pathogenesis mediated by microbiome metabolites
Tovaglieri, A., Sontheimer-Phelps, A., Geirnaert, A., Prantil‐Baun, R., Camacho, D. M., Chou, D. B., Jalili‐Firoozinezhad, S., de Wouters, T., Kasendra, M. J., Super, M., et al. (2019) · Microbiome · doi:10.1186/s40168-019-0650-5 · Cited by 140 · see citing works
A comparison of enteropathogenic and enterohaemorrhagicEscherichia colipathogenesis
Spears, K. J., Roe, A. J., & Gally, D. L. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2006.00119.x · Cited by 137 · see citing works
Mucus and Mucins: The Underappreciated Host Defence System
Sheng, Y., & Hasnain, S. Z. (2022) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2022.856962 · Cited by 137 · see citing works
Bacterial proteases in IBD and IBS
Steck, N., Mueller, K., Schemann, M., & Haller, D. (2011) · Gut · doi:10.1136/gutjnl-2011-300775 · Cited by 119 · see citing works
Display of the human mucinome with defined O-glycans by gene engineered cells
Nason, R., Büll, C., Konstantinidi, A., Sun, L., Ye, Z., Halim, A., Du, W., Sørensen, D. M., Durbesson, F., Furukawa, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24366-4 · Cited by 123 · see citing works
Bacterial Genetic Determinants of Non‐O157 STEC Outbreaks and Hemolytic‐Uremic Syndrome after Infection
Wickham, M. E., Lupp, C., Mascarenhas, M., Vázquez, A., Coombes, B. K., Brown, N. F., Coburn, B. A., Deng, W., Puente, J. L., Karmali, M. A., et al. (2006) · The Journal of Infectious Diseases · doi:10.1086/506620 · Cited by 117 · see citing works
The Genus Escherichia
Welch, R. A. (2006) · Springer eBooks · doi:10.1007/0-387-30746-x_3 · Cited by 109 · see citing works
Self-citation
Colonization resistance and microbial ecophysiology: using gnotobiotic mouse models and single-cell technology to explore the intestinal jungle
Stecher, B., Berry, D., & Loy, A. (2013) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12024 · Cited by 101 · see citing works
Interactions of commensal and pathogenic microorganisms with the mucus layer in the colon
Cai, R., Chen, C., Chen, J., Xu, X., Ding, C., & Gu, B. (2020) · Gut Microbes · doi:10.1080/19490976.2020.1735606 · Cited by 100 · see citing works
Change Is Good: Variations in Common Biological Mechanisms in the Epsilonproteobacterial GeneraCampylobacterandHelicobacter
Gilbreath, J. J., Cody, W. L., Merrell, D. S., & Hendrixson, D. R. (2011) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00035-10 · Cited by 94 · see citing works
The Vat-AIEC protease promotes crossing of the intestinal mucus layer by Crohn's disease-associatedEscherichia coli
Gibold, L., Garénaux, E., Dalmasso, G., Gallucci, C., Cia, D., Mottet-Auselo, B., Faïs, T., Darfeuille–Michaud, A., Nguyen, H. T. T., Barnich, N., et al. (2015) · Cellular Microbiology · doi:10.1111/cmi.12539 · Cited by 91 · see citing works
The emerging roles of bacterial proteases in intestinal diseases
Caminero, A., Guzman, M., Libertucci, J., & Lomax, A. E. (2023) · Gut Microbes · doi:10.1080/19490976.2023.2181922 · Cited by 92 · see citing works
Roadblocks in the gut: barriers to enteric infection
Gill, N., Wlodarska, M., & Finlay, B. B. (2011) · Cellular Microbiology · doi:10.1111/j.1462-5822.2011.01578.x · Cited by 87 · see citing works
TagA is a secreted protease of Vibrio cholerae that specifically cleaves mucin glycoproteins
Szabady, R. L., Yanta, J. H., Halladin, D. K., Schofield, M. J., & Welch, R. A. (2010) · Microbiology · doi:10.1099/mic.0.044529-0 · Cited by 87 · see citing works
Self-citation
EspP, a Type V-secreted serine protease of enterohaemorrhagicEscherichia coliO157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells
Dziva, F., Mahajan, A., Cameron, P., Currie, C., McKendrick, I. J., Wallis, T. S., Smith, D. G., & Stevens, M. P. (2007) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2007.00724.x · Cited by 83 · see citing works
Structural Insight into the Bacterial Mucinase StcE Essential to Adhesion and Immune Evasion during Enterohemorrhagic E. coli Infection
Yu, A. C. Y., Worrall, L. J., & Strynadka, N. C. J. (2012) · Structure · doi:10.1016/j.str.2012.02.015 · Cited by 83 · see citing works
Hemolytic uremic syndrome: pathogenesis and update of interventions
Palermo, M. S., Exeni, R. A., & Fernández, G. C. (2009) · Expert Review of Anti-infective Therapy · doi:10.1586/eri.09.49 · Cited by 79 · see citing works
Mucosal Inflammatory Response to Salmonella typhimurium Infection
Patel, S. N., & McCormick, B. A. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00311 · Cited by 78 · see citing works
The StcE metalloprotease of enterohaemorrhagicEscherichia colireduces the inner mucus layer and promotes adherence to human colonic epitheliumex vivo
Hews, C. L., Tran, S.-L., Wegmann, U., Brett, B., Walsham, A. D. S., Kavanaugh, D., Ward, N. J., Juge, N., & Schüller, S. (2017) · Cellular Microbiology · doi:10.1111/cmi.12717 · Cited by 77 · see citing works
Increased Adherence and Expression of Virulence Genes in a Lineage of Escherichia coli O157:H7 Commonly Associated with Human Infections
Abu-Ali, G., Ouellette, L., Henderson, S. T., Lacher, D. W., Riordan, J. T., Whittam, T. S., & Manning, S. D. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010167 · Cited by 76 · see citing works
Classification, structural biology, and applications of mucin domain-targeting proteases
Shon, D. J., Kuo, A., Ferracane, M. J., & Malaker, S. A. (2021) · Biochemical Journal · doi:10.1042/bcj20200607 · Cited by 72 · see citing works
Type 2 Secretion Promotes Enterohemorrhagic Escherichia coli Adherence and Intestinal Colonization
Ho, T. D., Davis, B. M., Ritchie, J. M., & Waldor, M. K. (2008) · Infection and Immunity · doi:10.1128/iai.01688-07 · Cited by 68 · see citing works
Pathogenic E. coli Exploits SslE Mucinase Activity to Translocate through the Mucosal Barrier and Get Access to Host Cells
Valeri, M., Paccani, S. R., Kasendra, M. J., Nesta, B., Serino, L., Pizza, M., & Soriani, M. (2015) · PLoS ONE · doi:10.1371/journal.pone.0117486 · Cited by 60 · see citing works
Differences in adherence and virulence gene expression between two outbreak strains of enterohaemorrhagic Escherichia coli O157 : H7
Abu-Ali, G., Ouellette, L., Henderson, S. T., Whittam, T. S., & Manning, S. D. (2009) · Microbiology · doi:10.1099/mic.0.033126-0 · Cited by 55 · see citing works
Modulation of Neutrophil Function by a Secreted Mucinase of Escherichia coli O157∶H7
Szabady, R. L., Lokuta, M. A., Walters, K., Anna Huttenlocher, A., & Welch, R. A. (2009) · PLoS Pathogens · doi:10.1371/journal.ppat.1000320 · Cited by 54 · see citing works
Self-citation
Differential Expression of Virulence and Stress Fitness Genes between Escherichia coli O157:H7 Strains with Clinical or Bovine-Biased Genotypes
Vanaja, S. K., Springman, A. C., Besser, T. E., Whittam, T. S., & Manning, S. D. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.01666-09 · Cited by 54 · see citing works
Modulation of Enterohaemorrhagic Escherichia coli Survival and Virulence in the Human Gastrointestinal Tract
Jubelin, G., Desvaux, M., Schüller, S., Etienne‐Mesmin, L., Muniesa, M., & Blanquet‐Diot, S. (2018) · Microorganisms · doi:10.3390/microorganisms6040115 · Cited by 54 · see citing works
The Glycoprotease CpaA Secreted by Medically Relevant Acinetobacter Species Targets Multiple O -Linked Host Glycoproteins
Haurat, M. F., Scott, N. E., Venanzio, G. D., Lopez, J., Pluvinage, B., Boraston, A. B., Ferracane, M. J., & Feldman, M. F. (2020) · mBio · doi:10.1128/mbio.02033-20 · Cited by 53 · see citing works
Streptococcus pyogenes antigen I/II‐family polypeptide AspA shows differential ligand‐binding properties and mediates biofilm formation
Maddocks, S., Wright, C. J., Nobbs, A. H., Brittan, J. L., Franklin, L., Strömberg, N., Kadioglu, A., Jepson, M. A., & Jenkinson, H. F. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07749.x · Cited by 52 · see citing works
Tripartite relationship between gut microbiota, intestinal mucus and dietary fibers: towards preventive strategies against enteric infections
Sauvaitre, T., Etienne‐Mesmin, L., Sivignon, A., Mosoni, P., Courtin, C. M., de Wiele, T. V., & Blanquet‐Diot, S. (2020) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuaa052 · Cited by 53 · see citing works
Characterization of the StcE Protease Activity of Escherichia coli O157:H7
Grys, T. E., Walters, L. L., & Welch, R. A. (2006) · Journal of Bacteriology · doi:10.1128/jb.01806-05 · Cited by 51 · see citing works
Self-citation
Pathogenic potential of a collagenase gene from Aeromonas veronii
Han, H., Taki, T., Kondo, H., Hırono, I., & Aoki, T. (2008) · Canadian Journal of Microbiology · doi:10.1139/w07-109 · Cited by 51 · see citing works
Microarray based comparison of two Escherichia coli O157:H7 lineages.
Dowd, S. E., & Ishizaki, H. (2006) · BMC Microbiology · doi:10.1186/1471-2180-6-30 · Cited by 51 · see citing works
Structural and mechanistic insights into the cleavage of clustered O-glycan patches-containing glycoproteins by mucinases of the human gut
Taleb, V., Liao, Q., Narimatsu, Y., García‐García, A., Compañón, I., Borges, R. J., González‐Ramírez, A. M., Corzana, F., Clausen, H., Rovira, C., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-32021-9 · Cited by 50 · see citing works
Type II secretion in Yersinia—a secretion system for pathogenicity and environmental fitness
von Tils, D., Blädel, I., Schmidt, M. A., & Heusipp, G. (2012) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2012.00160 · Cited by 49 · see citing works
Enterohemorrhagic Escherichia coli O157∶H7 Gene Expression Profiling in Response to Growth in the Presence of Host Epithelia
Jandu, N., Ho, N., Donato, K. A., Karmali, M. A., Mascarenhas, M., Duffy, S. P., Tailor, C. S., & Sherman, P. M. (2009) · PLoS ONE · doi:10.1371/journal.pone.0004889 · Cited by 47 · see citing works
YghG (GspS β ) Is a Novel Pilot Protein Required for Localization of the GspS β Type II Secretion System Secretin of Enterotoxigenic Escherichia coli
Strozen, T. G., Li, G., & Howard, S. P. (2012) · Infection and Immunity · doi:10.1128/iai.06394-11 · Cited by 47 · see citing works
Advances in glycopeptide enrichment methods for the analysis of protein glycosylation over the past decade
Li, J., Zhang, J., Xu, M., Yang, Z., Yue, S., Zhou, W., Gui, C., Zhang, H., Li, S., Wang, P. G., et al. (2022) · Journal of Separation Science · doi:10.1002/jssc.202200292 · Cited by 41 · see citing works
Retrospective Application of Transposon-Directed Insertion Site Sequencing to a Library of Signature-Tagged Mini-Tn 5 Km2 Mutants of Escherichia coli O157:H7 Screened in Cattle
Eckert, S. E., Dziva, F., Chaudhuri, R. R., Langridge, G. C., Turner, D., Pickard, D., Maskell, D. J., Thomson, N. R., & Stevens, M. P. (2011) · Journal of Bacteriology · doi:10.1128/jb.01292-10 · Cited by 40 · see citing works
Modeling mucus physiology and pathophysiology in human organs-on-chips
Izadifar, Z., Sontheimer-Phelps, A., Lubamba, B., Bai, H., Fadel, C. W., Stejskalová, A., Özkan, A., Dasgupta, Q., Bein, A., Junaid, A., et al. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114542 · Cited by 37 · see citing works
Effects of Muclin (Dmbt1) deficiency on the gastrointestinal system
Lisle, R. C. D., Xu, W., Roe, B. A., & Ziemer, D. M. (2008) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00525.2007 · Cited by 36 · see citing works
Virulence Gene Profiles and Population Genetic Analysis for Exploration of Pathogenic Serogroups of Shiga Toxin-Producing Escherichia coli
Kobayashi, N., Lee, K., Yamazaki, A., Saito, S., Furukawa, I., Kono, T., Maeda, E., Isobe, J., Sugita‐Konishi, Y., & Hara‐Kudo, Y. (2013) · Journal of Clinical Microbiology · doi:10.1128/jcm.01598-13 · Cited by 34 · see citing works
Microarray Analysis of the Ler Regulon in Enteropathogenic and Enterohaemorrhagic Escherichia coli Strains
Bingle, L. E. H., Constantinidou, C., Shaw, R. K., Islam, M. S., Patel, M. D., Snyder, L. A. S., Lee, D. J., Penn, C. W., Busby, S. J. W., & Pallen, M. J. (2014) · PLoS ONE · doi:10.1371/journal.pone.0080160 · Cited by 32 · see citing works
Enterohemorrhagic Escherichia coli Virulence Gene Regulation
Mellies, J. L., & Lorenzen, E. (2014) · Microbiology Spectrum · doi:10.1128/microbiolspec.ehec-0004-2013 · Cited by 30 · see citing works
Molecular and Functional Characterization of a ToxR-Regulated Lipoprotein from a Clinical Isolate ofAeromonas hydrophila
Pillai, L., Sha, J., Erova, T. E., Fadl, A. A., Khajanchi, B. K., & Chopra, A. K. (2006) · Infection and Immunity · doi:10.1128/iai.00402-06 · Cited by 29 · see citing works
Genome sequencing and comparative genomics of enterohemorrhagic Escherichia coli O145:H25 and O145:H28 reveal distinct evolutionary paths and marked variations in traits associated with virulence & colonization
Lorenz, S. C., González-Escalona, N., Kotewicz, M., Fischer, M., & Kase, J. (2017) · BMC Microbiology · doi:10.1186/s12866-017-1094-3 · Cited by 27 · see citing works
Regulation Mechanisms of Virulence Genes in Enterohemorrhagic Escherichia coli
Gelalcha, B., Brown, S. M., Crocker, H. E., Agga, G., & Dego, O. K. (2022) · Foodborne pathogens and disease · doi:10.1089/fpd.2021.0103 · Cited by 24 · see citing works
Differential Gene Expression and Adherence of Escherichia coli O157:H7 In Vitro and in Ligated Pig Intestines
Yin, X., Zhu, J., Feng, Y., Chambers, J. R., Gong, J., & Gyles, C. L. (2011) · PLoS ONE · doi:10.1371/journal.pone.0017424 · Cited by 27 · see citing works
Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene ( ehxA ) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path
Lorenz, S. C., Monday, S. R., Hoffmann, M., Fischer, M. J., & Kase, J. A. (2016) · Applied and Environmental Microbiology · doi:10.1128/aem.01839-16 · Cited by 25 · see citing works
Comparative genomics of Chinese and international isolates of Escherichia albertii: population structure and evolution of virulence and antimicrobial resistance
Luo, L., Wang, H., Payne, M., Liang, C., Bai, L., Zheng, H., Zhang, Z., Zhang, L., Zhang, X., Yan, G., et al. (2021) · Microbial Genomics · doi:10.1099/mgen.0.000710 · Cited by 24 · see citing works
Characterization of a QTL region affecting clinical mastitis and protein yield on BTA6
Nilsen, H., Olsen, H. G., Hayes, B. J., Nome, T., Sehested, E., Svendsen, M., Meuwissen, T. H. E., & Lien, S. (2009) · Animal Genetics · doi:10.1111/j.1365-2052.2009.01908.x · Cited by 24 · see citing works
Genetic Diversity among Escherichia coli O157:H7 Isolates and Identification of Genes Linked to Human Infections
Wu, G., Carter, B., Mafura, M. T., Liébana, E., Woodward, M. J., & Anjum, M. F. (2007) · Infection and Immunity · doi:10.1128/iai.00956-07 · Cited by 23 · see citing works
Pangenome analysis and virulence profiling of Streptococcus intermedius
Sinha, D. P., Sun, X., Khare, M., Drancourt, M., Raoult, D., & Fournier, P. (2021) · BMC Genomics · doi:10.1186/s12864-021-07829-2 · Cited by 22 · see citing works
Increased expression of deleted in malignant brain tumors (DMBT1) gene in precancerous gastric lesions: Findings from human and animal studies
Garay, J., Piazuelo, M. B., López‐Carrillo, L., Leal, Y. A., Majumdar, S., Li, L., Cruz-Rodríguez, N., Serrano-Gomez, S. J., Busso, C. S., Schneider, B., et al. (2017) · Oncotarget · doi:10.18632/oncotarget.16792 · Cited by 22 · see citing works
Characterizing the Escherichia coli O157:H7 Proteome Including Protein Associations with Higher Order Assemblies
Pieper, R., Zhang, Q., Clark, D. J., Huang, S.-T., Suh, M., Braisted, J. C., Payne, S., Fleischmann, R. D., Peterson, S. N., & Tzipori, S. R. (2011) · PLoS ONE · doi:10.1371/journal.pone.0026554 · Cited by 19 · see citing works
Enteropathogenic Infections: Organoids Go Bacterial
Hentschel, V., Arnold, F., Seufferlein, T. T. W., Azoitei, N., Kleger, A., & Müller, M. (2021) · Stem Cells International · doi:10.1155/2021/8847804 · Cited by 20 · see citing works
Genome Informatics and Machine Learning-Based Identification of Antimicrobial Resistance-Encoding Features and Virulence Attributes in Escherichia coli Genomes Representing Globally Prevalent Lineages, Including High-Risk Clonal Complexes
Shaik, S., Singh, A., Suresh, A., & Ahmed, N. (2022) · mBio · doi:10.1128/mbio.03796-21 · Cited by 20 · see citing works
Interactions between Enterohemorrhagic Escherichia coli (EHEC) and Gut Commensals at the Interface of Human Colonoids
Martins, F. H., Rajan, A., Carter, H. E., Baniasadi, H. R., Maresso, A. W., & Sperandio, V. (2022) · mBio · doi:10.1128/mbio.01321-22 · Cited by 21 · see citing works
Gene interaction network to unravel the role of gut bacterial species in cardiovascular diseases: E. coli O157:H7 host-bacterial interaction study
Miryala, S. K., Anbarasu, A., & Ramaiah, S. (2021) · Computers in Biology and Medicine · doi:10.1016/j.compbiomed.2021.104417 · Cited by 20 · see citing works
Genetic and Functional Analyses of Virulence Potential of an Escherichia coli O157:H7 Strain Isolated From Super-Shedder Cattle
Teng, L., Lee, S.-Y., Park, D.-J., & Jeong, K. C. (2020) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.00271 · Cited by 19 · see citing works
Proteomic View of Interactions of Shiga Toxin-Producing Escherichia coli with the Intestinal Environment in Gnotobiotic Piglets
Pieper, R., Zhang, Q., Clark, D. J., Parmar, P. P., Alami, H., Suh, M., Kuntumalla, S., Braisted, J. C., Huang, S., & Tzipori, S. R. (2013) · PLoS ONE · doi:10.1371/journal.pone.0066462 · Cited by 19 · see citing works
Bacterial warfare is associated with virulence and antimicrobial resistance
Sharp, C., & Foster, K. R. (2025) · Nature Communications · doi:10.1038/s41467-025-64363-5 · Cited by 19 · see citing works
Phage-encoded carbohydrate-interacting proteins in the human gut
Rothschild-Rodriguez, D., Hedges, M., Kaplan, M., Karav, S., & Nóbrega, F. L. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1083208 · Cited by 18 · see citing works
Adherence and associated virulence gene expression in acid-treated Escherichia coli O157 : H7 in vitro and in ligated pig intestine
Yin, X., Feng, Y., Yang, L., Chambers, J. R., Gong, J., & Gyles, C. L. (2012) · Microbiology · doi:10.1099/mic.0.056101-0 · Cited by 16 · see citing works
Variation in the Distribution of Putative Virulence and Colonization Factors in Shiga Toxin-Producing Escherichia coli Isolated from Different Categories of Cattle
Cáceres, M. E., Etcheverría, A. I., Fernández-García, D., Rodríguez, E. M., & Padola, N. L. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00147 · Cited by 16 · see citing works
Global Transcriptional and Phenotypic Analyses of Escherichia coli O157:H7 Strain Xuzhou21 and Its pO157_Sal Cured Mutant
Zhao, H., Chen, C., Xiong, Y., Xu, X., Lan, R., Wang, H., Yao, X., Bai, X., Liu, X., Meng, Q., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0065466 · Cited by 16 · see citing works
A Survey of Escherichia coli O157:H7 Virulence Factors: The First 25 Years and 13 Genomes
Reiland, H. A., Omolo, M. A., Johnson, T. J., & Baumler, D. J. (2014) · Advances in Microbiology · doi:10.4236/aim.2014.47046 · Cited by 15 · see citing works
The Secretome landscape of Escherichia coli O157:H7: Deciphering the cell-surface, outer membrane vesicle and extracellular subproteomes
Monteiro, R., Chafsey, I., Ageorges, V., Léroy, S., Chambon, C., Hébraud, M., Livrelli, V., Pizza, M., Pezzicoli, A., & Desvaux, M. (2020) · Journal of Proteomics · doi:10.1016/j.jprot.2020.104025 · Cited by 14 · see citing works
Crystal structure of the pilotin from the enterohemorrhagic Escherichia coli type II secretion system
Korotkov, K. V., & Hol, W. G. J. (2013) · Journal of Structural Biology · doi:10.1016/j.jsb.2013.02.013 · Cited by 14 · see citing works
DMBT1 as an archetypal link between infection, inflammation, and cancer
Mollenhauer, J., End, C., Renner, M., Lyer, S., & Poustka, A. (2007) · Inmunología · doi:10.1016/s0213-9626(07)70089-6 · Cited by 14 · see citing works
Stepwise assembly and release of Tc toxins from Yersinia entomophaga
Feldmüller, M., Ericson, C. F., Afanasyev, P., Lien, Y., Weiss, G. L., Wollweber, F., Schoof, M., Hurst, M., & Pilhofer, M. (2024) · Nature Microbiology · doi:10.1038/s41564-024-01611-2 · Cited by 13 · see citing works
Proteomes of pathogenicEscherichia coli/Shigellagroup surveyed in their host environments
Suh, M., Kuntumalla, S., Yu, Y., & Pieper, R. (2014) · Expert Review of Proteomics · doi:10.1586/14789450.2014.935342 · Cited by 13 · see citing works
Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins
Jaroentomeechai, T., Veloz, B., Soares, C. O., Goerdeler, F., Grosso, A. S., Büll, C., Miller, R. L., Furukawa, S., Ginés‐Alcober, I., Taleb, V., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-63756-w · Cited by 12 · see citing works
Screening of an E. coli O157:H7 Bacterial Artificial Chromosome Library by Comparative Genomic Hybridization to Identify Genomic Regions Contributing to Growth in Bovine Gastrointestinal Mucus and Epithelial Cell Colonization
Bai, J., McAteer, S. P., Paxton, E. A., Mahajan, A., Gally, D. L., & Tree, J. J. (2011) · Frontiers in Microbiology · doi:10.3389/fmicb.2011.00168 · Cited by 12 · see citing works
Enteropathogens: Tuning Their Gene Expression for Hassle-Free Survival
Chatterjee, R., Shreenivas, M. M., Sunil, R., & Chakravortty, D. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.03303 · Cited by 12 · see citing works
Interplay of flagellar motility and mucin degradation stimulates the uassociation of Pseudomonas aeruginosa with human epithelial colorectal adenocarcinoma (Caco-2) cells
Hayashi, N., Matsukawa, M., Horinishi, Y., Nakai, K., Shoji, A., Yoneko, Y., Yoshida, N., Minagawa, S., & Gotoh, N. (2013) · Journal of Infection and Chemotherapy · doi:10.1007/s10156-013-0554-4 · Cited by 12 · see citing works
Republished: Bacterial proteases in IBD and IBS
Steck, N., Mueller, K., Schemann, M., & Haller, D. (2012) · Postgraduate Medical Journal · doi:10.1136/postgradmedj-2011-300775rep · Cited by 10 · see citing works
Enterohemorrhagic and other Shigatoxin-producing Escherichia coli
Vanaja, S. K., Jandhyala, D. M., Mallick, E. M., Leong, J. M., & Balasubramanian, S. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-397048-0.00005-x · Cited by 10 · see citing works
Phosphotyrosine-Mediated Regulation of Enterohemorrhagic Escherichia coli Virulence
Robertson, C. D., Hazen, T. H., Kaper, J. B., Rasko, D. A., & Hansen, A. (2018) · mBio · doi:10.1128/mbio.00097-18 · Cited by 10 · see citing works
Plasma membrane profiling during enterohemorrhagic E. coli infection reveals that the metalloprotease StcE cleaves CD55 from host epithelial surfaces
Furniss, R. C. D., Low, W. W., Mavridou, D. A. I., Dagley, L. F., Webb, A. I., Tate, E. W., & Clements, A. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.005114 · Cited by 10 · see citing works
Type II Secretion in Escherichia coli
Patrick, M., Gray, M. D., Sandkvist, M., & Johnson, T. L. (2010) · EcoSal Plus · doi:10.1128/ecosalplus.4.3.4 · Cited by 10 · see citing works
Postinfectious Hemolytic Uremic Syndrome
Bitzan, M. M., & Lapeyraque, A. (2016) · Springer eBooks · doi:10.1007/978-3-662-52972-0_26 · Cited by 10 · see citing works
A family of di-glutamate mucin-degrading enzymes that bridges glycan hydrolases and peptidases
Narimatsu, Y., Büll, C., Taleb, V., Liao, Q., Compañón, I., Sánchez‐Navarro, D., Durbesson, F., Vincentelli, R., Hansen, L. H., Corzana, F., et al. (2024) · Nature Catalysis · doi:10.1038/s41929-024-01116-5 · Cited by 9 · see citing works

O157:H7 to Host Cells Escherichia coli Adherence of Enterohemorrhagic The StcE Protease Contributes to Intimate
Welch, R. A., Grys, T. E., Siegel, M. B., & Lathem, W. W. (2014)
Self-citation
Characterization of the StcE Protease Activity of Escherichia coli O157:H7
Grys, T. E., Walters, L. L., & Welch, R. A. (2006) · Journal of Bacteriology · doi:10.1128/jb.01806-05 · Cited by 51 · see citing works
Self-citation
StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor
Lathem, W. W., Grys, T. E., Witowski, S. E., Torres, A. G., Kaper, J. B., Tarr, P. I., & Welch, R. A. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02997.x · Cited by 183 · see citing works
Self-citation
The StcE metalloprotease of enterohaemorrhagicEscherichia colireduces the inner mucus layer and promotes adherence to human colonic epitheliumex vivo
Hews, C. L., Tran, S.-L., Wegmann, U., Brett, B., Walsham, A. D. S., Kavanaugh, D., Ward, N. J., Juge, N., & Schüller, S. (2017) · Cellular Microbiology · doi:10.1111/cmi.12717 · Cited by 77 · see citing works
Intestinal adherence associated with type IV pili of enterohemorrhagic Escherichia coli O157:H7
Xicohtencatl‐Cortés, J., Monteiro‐Neto, V., Ledesma, M. A., Jordan, D. M., Francetić, O., Kaper, J. B., Puente, J. L., & Girón, J. A. (2007) · Journal of Clinical Investigation · doi:10.1172/jci30727 · Cited by 135 · see citing works

Authors: Grys, T. E., Siegel, M. B., Lathem, W. W., & Welch, R. A.
Affiliations: University of Wisconsin–Madison (US)
ORCID: Grys, Thomas E.
Lathem, Wyndham W.
Welch, Rodney A.
Venue: Infection and Immunity
Publisher: American Society for Microbiology
Published: 2005-03
Volume: 73
Issue: 3
Pages: 1295-1303
ISSN: 0019-9567 (ISSN-L)
1098-5522
DOI: 10.1128/iai.73.3.1295-1303.2005
PMC: PMC1064933
OpenAlex: W2097638901
Cited by: 138
References: 72
Citations by year: 2026: 8 · 2025: 7 · 2024: 2 · 2023: 3 · 2022: 12 · 2021: 7 · 2020: 6 · 2019: 5 · 2018: 6 · 2017: 5 · 2016: 6 · 2015: 4 · 2014: 6 · 2013: 9 · 2012: 7
FWCI: 4.02
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Infection and Immunity (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
Funders: National Institutes of Health
National Institute of General Medical Sciences (5T32GM08349, T32GM008349)
National Institute of Allergy and Infectious Diseases (R01AI051735)
National Institute of Diabetes and Digestive and Kidney Diseases (R01DK063250)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
Keywords: Escherichia coli O157:H7, zinc metalloprotease, type II secretion system, C1 inhibitor, actin cytoskeleton, HEp-2 cells, saliva viscosity, attaching and effacing lesions
MeSH terms: Bacterial Adhesion, Cell Line, Humans, Metalloendopeptidases, Molecular Sequence Data, Mucins, Receptors, Immunologic, Saliva, Salivary Proteins and Peptides, Virulence, Sequence Analysis, DNA, Escherichia coli O157, Escherichia coli Proteins

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The StcE Protease Contributes to Intimate Adherence of Enterohemorrhagic Escherichia coli O157:H7 to Host Cells

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