Cited by 77Escherichia coli research studiesBacterial Genetics and BiotechnologyEnterobacteriaceae and Cronobacter Research

The StcE metalloprotease of enterohaemorrhagic Escherichia coli reduces the inner mucus layer and promotes adherence to human colonic epithelium ex vivo

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Hews, Claire L., Tran, Seav-Ly, Wegmann, Udo, Brett, Bernard, Walsham, Alistair D.S., Kavanaugh, Devon, Ward, Nicole J., Juge, Nathalie +1 more·2017·Cellular Microbiology

Abstract

Enterohaemorrhagic Escherichia coli (EHEC) is a major foodborne pathogen and tightly adheres to human colonic epithelium by forming attaching/effacing lesions. To reach the epithelial surface, EHEC must penetrate the thick mucus layer protecting the colonic epithelium. In this study, we investigated how EHEC interacts with the intestinal mucus layer using mucin-producing LS174T colon carcinoma cells and human colonic mucosal biopsies. The level of EHEC binding and attaching/effacing lesion formation in LS174T cells was higher compared to mucin-deficient colon carcinoma cell lines, and initial adherence was independent of the presence of flagellin, Escherichia coli common pilus, or long polar fimbriae. Although EHEC infection did not affect gene expression of secreted mucins, it resulted in reduced MUC2 glycoprotein levels. This effect was dependent on the catalytic activity of the secreted metalloprotease StcE, which reduced the inner mucus layer and thereby promoted EHEC access and binding to the epithelium in vitro and ex vivo. Given the lack of efficient therapies against EHEC infection, StcE may represent a suitable target for future treatment and prevention strategies.

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Crossref · 10.1111/cmi.12717

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  • StcE and SslE, metalloproteases from diarrheagenic E. coli strains, cleave mucin glycoproteins, which may help the pathogen to reach the epithelium.

    “StcE and SslE, metalloproteases from diarrheagenic E. coli strains, also cleave mucin glycoproteins, which may help the pathogen to reach the epithelium.74–76”
  • The zinc metalloproteases StcE and SslE secreted by E. coli EHEC and EPEC/ETEC respectively contribute to intimate adherence of these bacteria to host cells, a process essential for colonization.

    “Finally, the zinc metalloproteases StcE and SslE secreted by E. coli EHEC and EPEC/ETEC respectively, contribute to intimate adherence of these bacteria to host cells, a process that is essential for colonization.73–78”

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Authors
Hews, C. L., Tran, S.-L., Wegmann, U., Brett, B., Walsham, A. D. S., Kavanaugh, D., Ward, N. J., Juge, N., & Schüller, S.
Affiliations
  • University of East Anglia (GB)
  • Quadram Institute (GB)
  • Norfolk and Norwich University Hospital (GB)
Corresponding author
Schüller, Stephanie
Venue
Cellular Microbiology
Publisher
Wiley
Published
2017-02-15
Volume
19
Issue
6
Pages
e12717
ISSN
1462-5814 (ISSN-L)
OpenAlex
W2570203452
Cited by
77
References
46
Citations by year
2026: 4 · 2025: 9 · 2024: 16 · 2023: 5 · 2022: 7 · 2021: 5 · 2020: 12 · 2019: 9 · 2018: 8 · 2017: 2
FWCI
6.53
Type
journal article
Language
en
Open access
Locations
  • Cellular Microbiology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • UEA Digital Repository (University of East Anglia) (submittedVersion)
Funders
Medical Research Council (MR/J002062/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
Keywords
enterohemorrhagic Escherichia coli, metalloproteinases, mucus layer, MUC2, mucin, attaching and effacing lesions, bacterial adhesion, colonic epithelium, LS174T cells, secreted mucins, flagellin, host-pathogen interaction, therapeutic target
MeSH terms
Bacterial Adhesion, Cell Line, Colon, Escherichia coli Infections, Flagellin, Humans, Intestinal Mucosa, Metalloendopeptidases, Mucus, Fimbriae, Bacterial, Caco-2 Cells, HT29 Cells, Escherichia coli O157, Escherichia coli Proteins, Mucin-2

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