Bundle-Forming Pili and EspA Are Involved in Biofilm Formation by Enteropathogenic Escherichia coli
Abstract
Microcolony formation is one of the initial steps in biofilm development, and in enteropathogenic Escherichia coli (EPEC) it is mediated by several adhesins, including the bundle-forming pilus (BFP) and the EspA filament. Here we report that EPEC forms biofilms on plastic under static conditions and a flowthrough continuous culture system. The abilities of several EPEC isogenic mutants to form biofilms were assessed. Adhesins such as BFP and EspA, important in microcolony formation on epithelial cells, are also involved in bacterial aggregation during biofilm formation on abiotic surfaces. Mutants that do not express BFP or EspA form more-diffuse biofilms than does the wild type. We also determined, using gfp transcriptional fusions, that, consistent with the role of these adhesins in biofilms, the genes encoding BFP and EspA are expressed during biofilm formation. Finally, expression of espA is controlled by a quorum-sensing (QS) regulatory mechanism, and the EPEC qseA QS mutant also forms altered biofilms, suggesting that this signaling mechanism plays an important role in EPEC biofilm development. Taken together, these studies allowed us to propose a model of EPEC biofilm formation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Bfp plays a major role in EPEC microcolony formation, which leads to biofilm formation.
“Bfp plays a major role in EPEC microcolony formation, which in turn leads to biofilm formation [123].”
From Bacterial autoaggregation (2018) · cites this paper as [123]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
Datsenko, K., & Wanner, B. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.120163297 · Cited by 15,902 · see citing works
The complete genome sequence of Escherichia coli K-12.
Blattner, F., Plunkett, G., Bloch, C., Perna, N., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C., Mayhew, G., et al. (1997) · Science · doi:10.1126/science.277.5331.1453 · Cited by 7,996 · see citing works
Diarrheagenic Escherichia coli
Nataro, J. P., & Kaper, J. B. (1998) · Clinical Microbiology Reviews · doi:10.1128/cmr.11.1.142 · Cited by 5,109 · see citing works
The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W., & Greenberg, E. P. (1998) · Science · doi:10.1126/science.280.5361.295 · Cited by 3,396 · see citing works
Quantification of biofilm structures by the novel computer program comstat
Heydorn, A., Nielsen, A. T., Hentzer, M., Sternberg, C., Givskov, M., Ersbøll, B. K., & Molin, S. (2000) · Microbiology · doi:10.1099/00221287-146-10-2395 · Cited by 2,185 · see citing works
Genome sequence of enterohaemorrhagic Escherichia coli O157:H7
Perna, N. T., Plunkett, G., Burland, V., Mau, B., Glasner, J. D., Rose, D. J., Mayhew, G. F., Evans, P. S., Gregor, J., Kirkpatrick, H. A., et al. (2001) · Nature · doi:10.1038/35054089 · Cited by 2,035 · see citing works
Pratt, L. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01061.x · Cited by 1,641 · see citing works
Welch, R. A., Burland, V., Plunkett, G., Redford, P., Roesch, P. L., Rasko, D. A., Buckles, E. L., Liou, S., Boutin, A., Hackett, J. D., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.252529799 · Cited by 1,523 · see citing works
Cellular Control of the Synthesis and Activity of the Bacterial Luminescent System
Nealson, K. H., Platt, T., & Hastings, J. W. (1970) · Journal of Bacteriology · doi:10.1128/jb.104.1.313-322.1970 · Cited by 1,301 · see citing works
Hayashi, T. (2001) · DNA Research · doi:10.1093/dnares/8.1.11 · Cited by 1,266 · see citing works
Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells.
Kenny, B., Devinney, R., Stein, M., Reinscheid, D., Frey, E., & Finlay, B. (1997) · Cell · doi:10.1016/s0092-8674(00)80437-7 · Cited by 1,258 · see citing works
Jerse, A. E., Yu, J., Tall, B. D., & Kaper, J. B. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.20.7839 · Cited by 1,125 · see citing works
Donnenberg, M. S., & Kaper, J. B. (1991) · Infection and Immunity · doi:10.1128/iai.59.12.4310-4317.1991 · Cited by 1,085 · see citing works
Surette, M. G., Miller, M. B., & Bassler, B. L. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.4.1639 · Cited by 980 · see citing works
Bacteria–host communication: the language of hormones
Sperandio, V., Torres, A. G., Jarvis, B., Nataro, J. P., & Kaper, J. B. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1537100100 · Cited by 902 · see citing works
Self-citationQuorum sensing controls biofilm formation in Vibrio cholerae
Hammer, B. K., & Bassler, B. L. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03688.x · Cited by 888 · see citing works
Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation
Cucarella, C., Solano, C., Valle, J., Amorena, B., Lasa, Í., & Penadés, J. R. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.9.2888-2896.2001 · Cited by 874 · see citing works
Quorum-sensing regulators control virulence gene expression in Vibrio cholerae
Zhu, J., Miller, M. B., Vance, R. E., Dziejman, M., Bassler, B. L., & Mekalanos, J. J. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052694299 · Cited by 849 · see citing works
Moon, H. W., Whipp, S. C., Argenzio, R. A., Levine, M. M., & Giannella, R. A. (1983) · Infection and Immunity · doi:10.1128/iai.41.3.1340-1351.1983 · Cited by 846 · see citing works
Levine, M., Nalin, D., Hornick, R. B., Bergquist, E. J., Waterman, D., Young, C., Sotman, S. B., & Rowe, B. (1978) · The Lancet · doi:10.1016/s0140-6736(78)90299-4 · Cited by 686 · see citing works
Chilcott, G. S., & Hughes, K. T. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.4.694-708.2000 · Cited by 664 · see citing works
Danese, P. N., Pratt, L. A., & Kolter, R. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.12.3593-3596.2000 · Cited by 656 · see citing works
Dissecting virulence: Systematic and functional analyses of a pathogenicity island
Deng, W., Puente, J. L., Gruenheid, S., Li, Y., Vallance, B. A., Vázquez, A., León, J. B., Ibarra, J. A., O’Donnell, P. A., Metalnikov, P. S., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0400326101 · Cited by 626 · see citing works
Jarvis, K. G., Girón, J. A., Jerse, A. E., McDaniel, T. K., Donnenberg, M. S., & Kaper, J. B. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.17.7996 · Cited by 626 · see citing works
Steps in the development of a Vibrio cholerae El Tor biofilm
Watnick, P. I., & Kolter, R. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01624.x · Cited by 623 · see citing works
An Inducible Bundle-Forming Pilus of Enteropathogenic Escherichia coli
Girón, J. A., Ho, A. S.-Y., & Schoolnik, G. K. (1991) · Science · doi:10.1126/science.1683004 · Cited by 604 · see citing works
Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells
Scaletsky, I. C. A., SILVA, M. L. M., & Trabulsi, L. R. (1984) · Infection and Immunity · doi:10.1128/iai.45.2.534-536.1984 · Cited by 585 · see citing works
Self-citationKnutton, S., Rosenshine, I., Pallen, M. J., Nisan, I., Neves, B. C., Bain, C., Wolff, C., Gordon Dougan, B., & Frankel, G. M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.8.2166 · Cited by 578 · see citing works
Barrios, A. F. G., Zuo, R., Hashimoto, Y., Li, Y., Bentley, W. E., & Wood, T. K. (2005) · Journal of Bacteriology · doi:10.1128/jb.188.1.305-316.2006 · Cited by 545 · see citing works
Quorum Sensing-Dependent Biofilms Enhance Colonization in Vibrio cholerae
Zhu, J., & Mekalanos, J. J. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00295-8 · Cited by 532 · see citing works
Sperandio, V., Torres, A. G., & Kaper, J. B. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02803.x · Cited by 523 · see citing works
Self-citationSperandio, V., Mellies, J. L., Nguyen, W., Shin, S., & Kaper, J. B. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.26.15196 · Cited by 515 · see citing works
Self-citationValdivia, R. H., & Falkow, S. (1996) · Molecular Microbiology · doi:10.1046/j.1365-2958.1996.00120.x · Cited by 486 · see citing works
Bieber, D., Ramer, S. W., Wu, C.-Y., Murray, W. J., Tobe, T., Fernandez, R., & Schoolnik, G. K. (1998) · Science · doi:10.1126/science.280.5372.2114 · Cited by 465 · see citing works
Garmendia, J., Frankel, G. M., & Crepin, V. F. (2005) · Infection and Immunity · doi:10.1128/iai.73.5.2573-2585.2005 · Cited by 408 · see citing works
Mellies, J. L., Elliott, S. J., Sperandio, V., Donnenberg, M. S., & Kaper, J. B. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01473.x · Cited by 390 · see citing works
Self-citationDetection of an Adherence Factor of Enteropathogenic Escherichia coli with a DNA Probe
Nataro, J. P., Baldini, M. M., Kaper, J. B., et al. (1985) · The Journal of Infectious Diseases · doi:10.1093/infdis/152.3.560 · Cited by 389 · see citing works
Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli
Murphy, K. C., & Campellone, K. G. (2003) · BMC Molecular Biology · doi:10.1186/1471-2199-4-11 · Cited by 373 · see citing works
The flagella of enteropathogenic Escherichia coli mediate adherence to epithelial cells
Girón, J. A., Torres, A. G., Freer, E., et al. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.02899.x · Cited by 363 · see citing works
Elliott, S. J., Sperandio, V., Girón, J. A., et al. (2000) · Infection and Immunity · doi:10.1128/iai.68.11.6115-6126.2000 · Cited by 355 · see citing works
Self-citationRothbaum, R., McAdams, A. J., Giannella, R. A., et al. (1982) · Gastroenterology · doi:10.1016/s0016-5085(82)80342-9 · Cited by 350 · see citing works
Danese, P. N., Pratt, L. A., Dove, S. L., & Kolter, R. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.02008.x · Cited by 345 · see citing works
Levine, M. M., & Edelman, R. (1984) · Epidemiologic Reviews · doi:10.1093/oxfordjournals.epirev.a036274 · Cited by 331 · see citing works
Ryu, J., & Beuchat, L. R. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.1.247-254.2005 · Cited by 329 · see citing works
Sekiya, K., Ohishi, M., Ogino, T., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191378598 · Cited by 307 · see citing works
Watnick, P. I., Lauriano, C. M., Klose, K. E., et al. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02195.x · Cited by 302 · see citing works
A Role for the Mannose-Sensitive Hemagglutinin in Biofilm Formation by Vibrio cholerae El Tor
Watnick, P. I., Fullner, K. J., & Kolter, R. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.11.3606-3609.1999 · Cited by 297 · see citing works
Donnenberg, M. S., Girón, J. A., Nataro, J. P., et al. (1992) · Molecular Microbiology · doi:10.1111/j.1365-2958.1992.tb02210.x · Cited by 288 · see citing works
Donnenberg, M. S., Yu, J., & Kaper, J. B. (1993) · Journal of Bacteriology · doi:10.1128/jb.175.15.4670-4680.1993 · Cited by 284 · see citing works
[2] Molecular tools for study of biofilm physiology
Christensen, B. B., Sternberg, C., Andersen, J. B., Palmer, R. J., Jr., Nielsen, A. T., Givskov, M., & Molin, S. (1999) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(99)10004-1 · Cited by 264 · see citing works
Gomez-Duarte, O. G., & Kaper, J. B. (1995) · Infection and Immunity · doi:10.1128/iai.63.5.1767-1776.1995 · Cited by 263 · see citing works
Kenny, B., Lai, L., Finlay, B. B., et al. (1996) · Molecular Microbiology · doi:10.1111/j.1365-2958.1996.tb02619.x · Cited by 259 · see citing works
Kenny, B., & Finlay, B. B. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.17.7991 · Cited by 250 · see citing works
Ferrières, L., & Clarke, D. J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03815.x · Cited by 245 · see citing works
Cleary, J., Lai, L., Shaw, R. K., Straatman-Iwanowska, A. A., Donnenberg, M. S., Frankel, G. M., & Knutton, S. (2004) · Microbiology · doi:10.1099/mic.0.26740-0 · Cited by 235 · see citing works
Kenny, B., Abe, A., Stein, M., & Finlay, B. B. (1997) · Infection and Immunity · doi:10.1128/iai.65.7.2606-2612.1997 · Cited by 234 · see citing works
Bustamante, V. H., Santana, F. J., Calva, E., & Puente, J. L. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02209.x · Cited by 224 · see citing works
Sandkvist, M., Michel, L. O., Hough, L. P., Morales, V. M., Bagdasarian, M. M., Koomey, M., DiRita, V. J., & Bagdasarian, M. M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.22.6994-7003.1997 · Cited by 211 · see citing works
Antigen-43-Mediated Autoaggregation ofEscherichia coli Is Blocked by Fimbriation
Hasman, H., Chakraborty, T., & Klemm, P. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.16.4834-4841.1999 · Cited by 189 · see citing works
Hicks, S., Frankel, G. M., Kaper, J. B., Gordon Dougan, B., & Phillips, A. D. (1998) · Infection and Immunity · doi:10.1128/iai.66.4.1570-1578.1998 · Cited by 186 · see citing works
The Pathogenesis of Hemorrhagic Colitis Caused by Escherichia coli O157:H7 in Gnotobiotic Piglets
Tzipori, S., Wachsmuth, I. K., Chapman, C., et al. (1986) · The Journal of Infectious Diseases · doi:10.1093/infdis/154.4.712 · Cited by 178 · see citing works
Sperandio, V., Li, C. C., & Kaper, J. B. (2002) · Infection and Immunity · doi:10.1128/iai.70.6.3085-3093.2002 · Cited by 173 · see citing works
Self-citationTauschek, M., Gorrell, R. J., Strugnell, R. A., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.092152899 · Cited by 172 · see citing works
Lai, L. C., Wainwright, L. A., Stone, K. D., et al. (1997) · Infection and Immunity · doi:10.1128/iai.65.6.2211-2217.1997 · Cited by 171 · see citing works
Schembri, M. A., & Klemm, P. (2001) · Infection and Immunity · doi:10.1128/iai.69.3.1322-1328.2001 · Cited by 163 · see citing works
Knutton, S., Baldini, M. M., Kaper, J. B., et al. (1987) · Infection and Immunity · doi:10.1128/iai.55.1.78-85.1987 · Cited by 157 · see citing works
León, J. B., Bustamante, V. H., Flores‐Valdez, M. A., et al. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.23.7918-7930.2005 · Cited by 148 · see citing works
Tobe, T., Schoolnik, G. K., Sohel, I., et al. (1996) · Molecular Microbiology · doi:10.1046/j.1365-2958.1996.531415.x · Cited by 138 · see citing works
Shin, S., Castanié‐Cornet, M., Foster, J. W., et al. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02570.x · Cited by 137 · see citing works
Modulation of Enteropathogenic Escherichia coli Virulence by Quorum Sensing
Sircili, M. P., Walters, M., Trabulsi, L. R., & Sperandio, V. (2004) · Infection and Immunity · doi:10.1128/iai.72.4.2329-2337.2004 · Cited by 127 · see citing works
Self-citationDifferential Expression of the <i>Escherichia</i> <i>coli</i> Autoaggregation Factor Antigen 43
Schembri, M. A., Hjerrild, L., Gjermansen, M., & Klemm, P. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.7.2236-2242.2003 · Cited by 126 · see citing works
[3] Quorum sensing signals in development of Pseudomonas aeruginosa biofilms
Parsek, M. R., & Greenberg, E. P. (1999) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(99)10005-3 · Cited by 124 · see citing works
Interaction of Ler at the LEE5 ( tir ) Operon of Enteropathogenic Escherichia coli
Haack, K. R., Robinson, C. L., Miller, K. J., et al. (2002) · Infection and Immunity · doi:10.1128/iai.71.1.384-392.2003 · Cited by 123 · see citing works
Pseudomonas aeruginosa attachment and biofilm development in dynamic environments
Ramsey, M. M., & Whiteley, M. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04181.x · Cited by 122 · see citing works
Self-citationSánchez‐SanMartín, C., Bustamante, V. H., Calva, E., et al. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.9.2823-2833.2001 · Cited by 108 · see citing works
Creasey, E. A., Delahay, R. M., Daniell, S. J., et al. (2003) · Microbiology · doi:10.1099/mic.0.26355-0 · Cited by 108 · see citing works
Advances in Burkholderia cepacia complex
Speert, D. P. (2002) · Paediatric Respiratory Reviews · doi:10.1016/s1526-0542(02)00185-9 · Cited by 90 · see citing works
Enteropathogenic Escherichia coli and life threatening chronic diarrhoea.
Hill, S., Phillips, A. D., & Walker-Smith, J. A. (1991) · Gut · doi:10.1136/gut.32.2.154 · Cited by 84 · see citing works
Leverton, L. Q., & Kaper, J. B. (2005) · Infection and Immunity · doi:10.1128/iai.73.2.1034-1043.2005 · Cited by 80 · see citing works
Creasey, E. A., Friedberg, D., Shaw, R. K., et al. (2003) · Microbiology · doi:10.1099/mic.0.26735-0 · Cited by 72 · see citing works
Ali, A., Johnson, J. A., Franco, A. A., et al. (2000) · Infection and Immunity · doi:10.1128/iai.68.4.1967-1974.2000 · Cited by 64 · see citing works
λ red-dependent growth and recombination of phage P22
Poteete, A. R., & Fenton, A. C. (1984) · Virology · doi:10.1016/0042-6822(84)90281-2 · Cited by 61 · see citing works
A SHORT HISTORY OF THE DEVELOPMENT OF THE BIOFILM CONCEPT
Costerton, J. W. (2004) · doi:10.1128/9781555817718.ch1 · Cited by 56 · see citing works
Nagata, T., Mukae, H., Kadota, J., et al. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.6.2251-2259.2004 · Cited by 44 · see citing works
Ibarra, J. A., Villalba, M. I., & Puente, J. L. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.9.2835-2847.2003 · Cited by 43 · see citing works
Factors affecting production of extracellular carbohydrate complexes by Escherichia coli O157:H7
Ryu, J., & Beuchat, L. R. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.02.014 · Cited by 26 · see citing works
Ryu, J., & Beuchat, L. R. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.02103.x · Cited by 10 · see citing works
How the bacterial flora and the epithelial cell get along
Sperandio, V. (2000) · Trends in Microbiology · doi:10.1016/s0966-842x(00)01896-5 · Cited by 5 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 1
- [64]64. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Cited by
Sorted by citations
Trunk, T., Bacterial Cell Surface Group, S. F. G. A. E. B., S. Khalil, H., & C. Leo, J. (2018) · AIMS Microbiology · doi:10.3934/microbiol.2018.1.140 · Cited by 444 · see citing works
“Bfp plays a major role in EPEC microcolony formation, which in turn leads to biofilm formation [123].”
BackgroundCampylobacter flagella: not just for motility
Guerry, P. (2007) · Trends in Microbiology · doi:10.1016/j.tim.2007.09.006 · Cited by 339 · see citing works
QseC Inhibitors as an Antivirulence Approach for Gram-Negative Pathogens
Curtis, M. M., Russell, R., Moreira, C. G., Adebesin, A. M., Wang, C., Williams, N. S., Taussig, R., Stewart, D., Zimmern, P., Lu, B., et al. (2014) · mBio · doi:10.1128/mbio.02165-14 · Cited by 136 · see citing works
Self-citation
- Authors
- Moreira, C. G., Palmer, K., Whiteley, M., Sircili, M. P., Trabulsi, L. R., Castro, A. F. P., & Sperandio, V.
- Affiliations
- Universidade de São Paulo (BR)
- University of Oklahoma Health Sciences Center (US)
- Instituto Butantan, Sao Paulo, Brazil 05503-900
- Dept. Microbiologia, Universidade de Sao Paulo, Sao Paulo, Brazil 05508-900
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Sperandio, Vanessa
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2006-06
- Volume
- 188
- Issue
- 11
- Pages
- 3952-3961
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2134912815
- Cited by
- 77
- References
- 88
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2022: 1 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 6 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 5 · 2012: 6
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- National Institutes of Health (AI053067)
- National Institute of Allergy and Infectious Diseases (R01AI053067, AI-054468, R21AI054468, AI 053067, R37AI053067)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- enteropathogenic Escherichia coli, biofilm formation, bundle-forming pilus, adhesins, microcolony formation, bacterial aggregation, abiotic surfaces, quorum sensing, gene expression regulation
- MeSH terms
- Escherichia coli, Genotype, Kinetics, Fimbriae, Bacterial, Recombinant Proteins, Transcription, Genetic, Signal Transduction, Mutagenesis, Biofilms, Escherichia coli Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
