Differential Expression of the Escherichia coli Autoaggregation Factor Antigen 43
Open accessAbstract
Antigen 43 (Ag43) is a self-recognizing surface adhesin found in most Escherichia coli strains. Due to its excellent cell-to-cell aggregation characteristics, Ag43 expression confers clumping and fluffing of cells and promotes biofilm formation. Ag43 expression is repressed by the cellular redox sensor OxyR. Here we used mutant versions of OxyR that are locked in either the reduced or the oxidized form as well as the addition of a simple redox-changing chemical to show that the redox state of OxyR influences Ag43 expression. Furthermore, the redox state of OxyR influences the biofilm-forming potential of E. coli. Finally, we demonstrated that Ag43-mediated cell aggregation confers significant protection against hydrogen peroxide killing.
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Ag43-mediated aggregation provides a mechanism for reducing local oxygen concentrations, thereby conferring high protection against oxidizing agents and H2O2 killing.
“Ag43 is a SAAT and Ag43-mediated aggregation provides a mechanism for reducing local oxygen concentrations, thereby conferring a high protection against oxidizing agents and H2O2 killing.”
From Bacterial autoaggregation (2018) · cites this paper as [8]
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“However, Ag43 expression does not appear to be directly linked to H2O2-induced stress and Ag43 mediated protection against H2O2killing is believed to be a side effect of Ag43 mediated aggregation [8].”
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- Authors
- Schembri, M. A., Hjerrild, L., Gjermansen, M., & Klemm, P.
- Affiliations
- Technical University of Denmark (DK)
- Corresponding author
- Klemm, Per
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2003-04
- Volume
- 185
- Issue
- 7
- Pages
- 2236-2242
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC151503
- OpenAlex
- W1613432855
- Cited by
- 126
- References
- 36
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 4 · 2023: 9 · 2022: 10 · 2021: 8 · 2020: 4 · 2019: 6 · 2018: 2 · 2017: 6 · 2016: 3 · 2015: 4 · 2014: 5 · 2013: 5 · 2012: 4
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Funders
- Teknologi og Produktion, Det Frie Forskningsråd
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- antigen 43, Escherichia coli, OxyR, redox regulation, cell aggregation, biofilm formation, surface adhesins, oxidative stress, hydrogen peroxide resistance, protein expression regulation
- MeSH terms
- Antigens, Bacterial, Bacterial Adhesion, Bacterial Outer Membrane Proteins, Dithiothreitol, DNA-Binding Proteins, Escherichia coli, Hydrogen Peroxide, Oxidation-Reduction, Promoter Regions, Genetic, Repressor Proteins, Transcription Factors, Protein Engineering, Gene Expression Regulation, Bacterial, Oxidants, Biofilms, Adhesins, Bacterial, Adhesins, Escherichia coli, Reducing Agents, Escherichia coli Proteins
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