Proteomic Analysis of the Pseudomonas aeruginosa Iron Starvation Response Reveals PrrF Small Regulatory RNA-Dependent Iron Regulation of Twitching Motility, Amino Acid Metabolism, and Zinc Homeostasis Proteins

Nelson, Cassandra E.; Huang, Weiliang; Brewer, Luke K.; Nguyen, Angela T.; Kane, Maureen A.; Wilks, Angela; Oglesby-Sherrouse, Amanda G.

doi:10.1128/jb.00754-18

Cited by 86

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Proteomic Analysis of the Pseudomonas aeruginosa Iron Starvation Response Reveals PrrF Small Regulatory RNA-Dependent Iron Regulation of Twitching Motility, Amino Acid Metabolism, and Zinc Homeostasis Proteins

Nelson, Cassandra E., Huang, Weiliang, Brewer, Luke K., Nguyen, Angela T., Kane, Maureen A., Wilks, Angela, Oglesby-Sherrouse, Amanda G.·2019·Journal of Bacteriology

DOI 10.1128/jb.00754-18

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Abstract

Iron is central for growth and metabolism of almost all microbial pathogens, and as such, this element is sequestered by the host innate immune system to restrict microbial growth. Here, we used label-free proteomics to investigate the Pseudomonas aeruginosa iron starvation response, revealing a broad landscape of metabolic and metal homeostasis changes that have not previously been described. We further provide evidence that many of these processes, including twitching motility, are regulated through the iron-responsive PrrF small regulatory RNAs. As such, this study demonstrates the power of proteomics for defining stress responses of microbial pathogens.

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Authors: Nelson, C. E., Huang, W., Brewer, L. K., Nguyen, A. T., Kane, M. A., Wilks, A., & Oglesby-Sherrouse, A. G.
Venue: Journal of Bacteriology
Publisher: American Society for Microbiology
Published: 2019-06-15
Volume: 201
Issue: 12
ISSN: 0021-9193
1098-5530
DOI: 10.1128/jb.00754-18
Cited by: 86
References: 17
Language: en
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Funders: HHS | National Institutes of Health (T32 AI095190, T32 GM066706, R01 AI123320, R01 HD077260, R01 AI134886)

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This page: CoChat. (2026). Citation check: Proteomic Analysis of the Pseudomonas aeruginosa Iron Starvation Response Reveals PrrF Small Regulatory RNA-Dependent Iron Regulation of Twitching Motility, Amino Acid Metabolism, and Zinc Homeostasis Proteins. https://app.cochat.ai/papers/10.1128/jb.00754-18
The paper: Nelson, C. E., Huang, W., Brewer, L. K., Nguyen, A. T., Kane, M. A., Wilks, A., & Oglesby-Sherrouse, A. G. (2019). Proteomic Analysis of the Pseudomonas aeruginosa Iron Starvation Response Reveals PrrF Small Regulatory RNA-Dependent Iron Regulation of Twitching Motility, Amino Acid Metabolism, and Zinc Homeostasis Proteins. Journal of Bacteriology. https://doi.org/10.1128/jb.00754-18

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Proteomic Analysis of the Pseudomonas aeruginosa Iron Starvation Response Reveals PrrF Small Regulatory RNA-Dependent Iron Regulation of Twitching Motility, Amino Acid Metabolism, and Zinc Homeostasis Proteins

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