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Coordinated Regulation by AgrA, SarA, and SarR To Control agr Expression in Staphylococcus aureus

Reyes, Dindo, Andrey, Diego O., Monod, Antoinette, Kelley, William L., Zhang, Gongyi, Cheung, Ambrose L.·2011·Journal of Bacteriology
DOI 10.1128/jb.05436-11
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Abstract

The agr locus of Staphylococcus aureus is composed of two divergent transcripts (RNAII and RNAIII) driven by the P2 and P3 promoters. The P2-P3 intergenic region comprises the SarA/SarR binding sites and the four AgrA boxes to which AgrA binds. We reported here the role of AgrA, SarA, and SarR on agr P2 and P3 transcription. Using real-time reverse transcription (RT)-PCR and promoter fusion studies with selected single, double, triple, and complemented mutants, we showed that AgrA is indispensable to agr P2 and P3 transcription, whereas SarA activates and SarR represses P2 transcription. In vitro runoff transcription assays revealed that AgrA alone promoted transcription from the agr P2 promoter, with SarA enhancing it and SarR inhibiting agr P2 transcription in the presence of AgrA or with SarA and AgrA. Electrophoretic mobility shift assay (EMSA) analysis disclosed that SarR binds more avidly to the agr promoter than SarA and displaces SarA from the agr promoter. Additionally, SarA and AgrA bend the agr P2 promoter, whereas SarR does not. Collectively, these data indicated that AgrA activates agr P2 and P3 promoters while SarA activates the P2 promoter, presumably via bending of promoter DNA to bring together AgrA dimers to facilitate engagement of RNA polymerase (RNAP) to initiate transcription.

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Metadata

Authors
Reyes, D., Andrey, D. O., Monod, A., Kelley, W. L., Zhang, G., & Cheung, A. L.
Affiliations
  • Department of Microbiology, Dartmouth Medical School, Hanover, New Hampshire 03755
  • Service of Infectious Diseases, University Hospital and Medical School of Geneva, 4 Rue Gabrielle-Perret-Gentil, CH-1211 Geneva 14, Switzerland
  • Department of Immunology, National Jewish Hospital, Denver, Colorado
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
2011-11
Volume
193
Issue
21
Pages
6020-6031
ISSN
  • 0021-9193
  • 1098-5530
DOI
10.1128/jb.05436-11
Cited by
126
References
1
Language
en
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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CoChat. (2026). Citation check: Coordinated Regulation by AgrA, SarA, and SarR To Control agr Expression in Staphylococcus aureus. https://app.cochat.ai/papers/10.1128/jb.05436-11
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Reyes, D., Andrey, D. O., Monod, A., Kelley, W. L., Zhang, G., & Cheung, A. L. (2011). Coordinated Regulation by AgrA, SarA, and SarR To Control agr Expression in Staphylococcus aureus. Journal of Bacteriology. https://doi.org/10.1128/jb.05436-11

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