The VanS-VanR two-component regulatory system controls synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147
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Plasmid pIP816 of Enterococcus faecium BM4147 confers inducible resistance to vancomycin and encodes the VanH dehydrogenase and the VanA ligase for synthesis of depsipeptide-containing peptidoglycan precursors which bind the antibiotic with reduced affinity. We have characterized a cluster of five genes of pIP816 sufficient for peptidoglycan synthesis in the presence of vancomycin. The distal part of the van cluster encodes VanH, VanA, and a third enzyme, VanX, all of which are necessary for resistance. Synthesis of these enzymes was regulated at the transcriptional level by the VanS-VanR two-component regulatory system encoded by the proximal part of the cluster. VanR was a transcriptional activator related to response regulators of the OmpR subclass. VanS stimulated VanR-dependent transcription and was related to membrane-associated histidine protein kinases which control the level of phosphorylation of response regulators. Analysis of transcriptional fusions with a reporter gene and RNA mapping indicated that the VanR-VanS two-component regulatory system activates a promoter used for cotranscription of the vanH, vanA, and vanX resistance genes.
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This initial step results in ATP-dependent phosphorylation of VanR, which subsequently binds to two promoters, one upstream of vanR and the other upstream of vanH in Tn1546.
“This initial step results in an ATP-dependent phosphorylation of the response regulator VanR, which subsequently binds to two promoters, one of them located upstream of its own gene (vanR) and the other upstream of vanH in Tn1546 (78).”
From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [78]
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Self-citationArbeloa, A., Segal, H., Hugonnet, J., Josseaume, N., Dubost, L., Brouard, J., Gutmann, L., Mengin‐Lecreulx, D., & Arthur, M. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.5.1221-1228.2004 · Cited by 137 · see citing works
Self-citationPhoP, a key player in Mycobacterium tuberculosis virulence
Ryndak, M. B., Wang, S., & Smith, I. (2008) · Trends in Microbiology · doi:10.1016/j.tim.2008.08.006 · Cited by 137 · see citing works
Fitness Cost of VanA-Type Vancomycin Resistance in Methicillin-Resistant Staphylococcus aureus
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Self-citationEukaryotic signal transduction via histidine-aspartate phosphorelay
Thomason, P. A., & Kay, R. R. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.18.3141 · Cited by 136 · see citing works
Histidine kinases as targets for new antimicrobial agents
Matsushita, M., & Janda, K. D. (2002) · Bioorganic & Medicinal Chemistry · doi:10.1016/s0968-0896(01)00355-8 · Cited by 136 · see citing works
Arthur, M., Molinas, C., Bugg, T. D. H., Wright, G. D., Walsh, C. T., & Courvalin, P. M. (1992) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.36.4.867 · Cited by 136 · see citing works
Self-citationA vancomycin photoprobe identifies the histidine kinase VanSsc as a vancomycin receptor
Koteva, K. K., Hong, H., Wang, X. D., Nazi, I., Hughes, D. W., Naldrett, M. J., Buttner, M. J., & Wright, G. D. (2010) · Nature Chemical Biology · doi:10.1038/nchembio.350 · Cited by 135 · see citing works
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Self-citationGlycopeptide-resistant enterococci: a decade of experience
Woodford, N. (1998) · Journal of Medical Microbiology · doi:10.1099/00222615-47-10-849 · Cited by 131 · see citing works
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Enterococci Acquire New Kinds of Resistance
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Environmental strains of Enterococcus faecium with inducible high-level resistance to glycopeptides
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vanC Cluster of Vancomycin-Resistant Enterococcus gallinarum BM4174
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Synthesis, antimicrobial activity and binding properties of calix[4]arene based vancomycin mimics
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- Authors
- Arthur, M., Molinas, C., & Courvalin, P.
- Affiliations
- Institut Pasteur (FR)
- ORCID
- Arthur, M
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1992-04
- Volume
- 174
- Issue
- 8
- Pages
- 2582-2591
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC205897
- OpenAlex
- W1628177336
- Cited by
- 338
- References
- 49
- Citations by year
- 2026: 5 · 2025: 11 · 2024: 4 · 2023: 7 · 2022: 12 · 2021: 11 · 2020: 7 · 2019: 4 · 2018: 10 · 2017: 7 · 2016: 11 · 2015: 4 · 2014: 15 · 2013: 5 · 2012: 4
- FWCI
- 9.85
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- vancomycin resistance, VanX, peptidoglycan biosynthesis, transcriptional regulation, response regulator, histidine kinase, antibiotic resistance genes
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Base Sequence, Cloning, Molecular, DNA Mutational Analysis, DNA, Bacterial, Drug Resistance, Microbial, Genes, Bacterial, Genes, Regulator, Molecular Sequence Data, Oligodeoxyribonucleotides, Peptidoglycan, Promoter Regions, Genetic, Protein Kinases, Transcription Factors, Transcription, Genetic, Vancomycin, Polymerase Chain Reaction, Sequence Alignment, Enterococcus faecium
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