The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Targeted PCR for Detection of Vaginal Bacteria Associated with Bacterial Vaginosis
Open accessAbstract
Several novel bacterial species have been detected in subjects with bacterial vaginosis (BV) by using broad-range PCR assays, but this approach is insensitive for detecting minority species. We developed a series of taxon-directed 16S rRNA gene PCR assays for more sensitive detection of key vaginal bacteria. We sought to determine the prevalence of each species in the vagina, its association with BV, and the utility of PCR for the microbiological diagnosis of BV. Targeted PCR assays were developed for 17 vaginal bacterial species and applied to 264 vaginal-fluid samples from 81 subjects with and 183 subjects without BV. The results were compared to those of two widely accepted methods for diagnosing BV, the use of clinical findings (Amsel criteria) and the interpretation of vaginal-fluid Gram stains (Nugent criteria). Leptotrichia / Sneathia , Atopobium vaginae , an Eggerthella -like bacterium, Megasphaera species, and three novel bacteria in the order Clostridiales are among the bacterial species significantly associated with BV. PCR detection of either a Megasphaera species or one of the Clostridiales bacteria yielded a sensitivity of 99% and a specificity of 89% for diagnosis of BV compared to the Amsel clinical criteria and a sensitivity of 95.9% and a specificity of 93.7% compared to the Nugent criteria (Gram stain). PCR detection of one or more fastidious bacterial species is a more reliable indicator of BV than detection of bacteria, such as Gardnerella vaginalis , previously linked to BV, highlighting the potential of PCR for the diagnosis of BV.
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Cites
Nugent, R. P., Krohn, M. A., & Hillier, S. L. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.2.297-301.1991 · Cited by 3,331
Amsel, R., Totten, P. A., Spiegel, C. A., Chen, K. C. S., Eschenbach, D., & Holmes, K. K. (1983) · The American Journal of Medicine · doi:10.1016/0002-9343(83)91112-9 · Cited by 2,349
Molecular Identification of Bacteria Associated with Bacterial Vaginosis
Fredricks, D. N., Fiedler, T. L., & Marrazzo, J. M. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa043802 · Cited by 1,303
Self-citationMartin, H. L., Richardson, B. A., Nyange, P. M., Lavreys, L., Hillier, S. L., Chohan, B., Mandaliya, K., Ndinya‐Achola, J. O., Bwayo, J., & Kreiss, J. (1999) · The Journal of Infectious Diseases · doi:10.1086/315127 · Cited by 891
Bacterial vaginosis as a risk factor for preterm delivery: A meta-analysis
Leitich, H., Bodner-Adler, B., Brunbauer, M., Kaider, A., Egarter, C., & Husslein, P. (2003) · American Journal of Obstetrics and Gynecology · doi:10.1067/mob.2003.339 · Cited by 736
Verhelst, R., Verstraelen, H., Claeys, G., Verschraegen, G., Delanghe, J., Van Simaey, L., De Ganck, C., Temmerman, M., & Vaneechoutte, M. (2004) · BMC Microbiology · doi:10.1186/1471-2180-4-16 · Cited by 348
Diagnostic Criteria and Microbial and Epidemiologic Associations
Amsel, R., Totten, P. A., Spiegel, C. A., Chen, K. C. S., & Holmes, K. K. (1983) · Cited by 267
Ferris, M. J., Masztal, A., Aldridge, K. E., Fortenberry, J. D., Fidel, P. L., & Martin, D. H. (2004) · BMC Infectious Diseases · doi:10.1186/1471-2334-4-5 · Cited by 258
Diagnostic microbiology of bacterial vaginosis
Hillier, S. L. (1993) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(93)90340-o · Cited by 255
Ison, C. A. (2002) · Sexually Transmitted Infections · doi:10.1136/sti.78.6.413 · Cited by 241
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Sexually Transmitted Infections Treatment Guidelines, 2021
Workowski, K. A., Bachmann, L. H., Chan, P. A., Johnston, C. M., Muzny, C. A., Park, I., Reno, H., Zenilman, J. M., & Bolan, G. A. (2021) · MMWR. Recommendations and Reports · doi:10.15585/mmwr.rr7004a1 · Cited by 3,018
The vaginal microbiome and preterm birth
Fettweis, J. M., Serrano, M. G., Brooks, J. P., Edwards, D. J., Girerd, P. H., Parikh, H. I., Huang, B., Arodz, T. J., Edupuganti, L., Glascock, A. L., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0450-2 · Cited by 983
Romero, R., Hassan, S. S., Gajer, P., Tarca, A. L., Fadrosh, D. W., Nikita, L., Galuppi, M., Lamont, R. F., Chaemsaithong, P., Miranda, J., et al. (2014) · Microbiome · doi:10.1186/2049-2618-2-4 · Cited by 852
Srinivasan, S., Hoffman, N. G., Morgan, M. T., Matsen, F. A., Fiedler, T. L., Hall, R. W., Ross, F. J., McCoy, C. O., Bumgarner, R., Marrazzo, J. M., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037818 · Cited by 673
Self-citationStapleton, A. E., Au-Yeung, M., Hooton, T. M., Fredricks, D. N., Roberts, P. L., Czaja, C. A., Yarova-Yarovaya, Y., Fiedler, T., Cox, M., & Stamm, W. E. (2011) · Clinical Infectious Diseases · doi:10.1093/cid/cir183 · Cited by 484
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Metadata
- Authors
- Fredricks, D. N., Fiedler, T. L., Thomas, K. K., Oakley, B. B., & Marrazzo, J. M.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2007-10
- Volume
- 45
- Issue
- 10
- Pages
- 3270-3276
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- OpenAlex
- W2137061941
- Cited by
- 406
- References
- 19
- FWCI
- 14.94
- Type
- journal article
- Language
- en
- Open access
- green
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