The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults
Open accessAbstract
Although Streptococcus mutans has been implicated as a major etiological agent of dental caries, our cross-sectional preliminary study indicated that 10% of subjects with rampant caries in permanent teeth do not have detectable levels of S. mutans . Our aims were to use molecular methods to detect all bacterial species associated with caries in primary and permanent teeth and to determine the bacterial profiles associated with different disease states. Plaque was collected from 39 healthy controls and from intact enamel and white-spot lesions, dentin lesions, and deep-dentin lesions in each of 51 subjects with severe caries. 16S rRNA genes were PCR amplified, cloned, and sequenced to determine species identities. In a reverse-capture checkerboard assay, 243 samples were analyzed for 110 prevalent bacterial species. A sequencing analysis of 1,285 16S rRNA clones detected 197 bacterial species/phylotypes, of which 50% were not cultivable. Twenty-two new phylotypes were identified. PROC MIXED tests revealed health- and disease-associated species. In subjects with S. mutans , additional species, e.g., species of the genera Atopobium , Propionibacterium , and Lactobacillus , were present at significantly higher levels than those of S. mutans . Lactobacillus spp., Bifidobacterium dentium , and low-pH non- S. mutans streptococci were predominant in subjects with no detectable S. mutans . Actinomyces spp. and non- S. mutans streptococci were predominant in white-spot lesions, while known acid producers were found at their highest levels later in disease. Bacterial profiles change with disease states and differ between primary and secondary dentitions. Bacterial species other than S. mutans , e.g., species of the genera Veillonella , Lactobacillus , Bifidobacterium , and Propionibacterium , low-pH non- S. mutans streptococci, Actinomyces spp., and Atopobium spp., likely play important roles in caries progression.
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“Commensal microbiota comprise the majority of cells in the body and play a key role in human health1-5,10.”
From Sequencing ancient calcified dental plaque shows changes in oral microbiota with dietary shifts of the Neolithic and Industrial revolutions (2013) · cites this paper as [1]
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Occlusal Caries: Biological Approach for Its Diagnosis and Management
Carvalho, J. C., Dige, I., Mačhiulskienė, V., Qvist, V., Bakhshandeh, A., Parolo, C. C. F., & Maltz, M. (2016) · Caries Research · doi:10.1159/000448662 · Cited by 2,159
Bacterial Diversity in Human Subgingival Plaque
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Ecological Hypothesis of Dentin and Root Caries
Takahashi, N., & Nyvad, B. (2016) · Caries Research · doi:10.1159/000447309 · Cited by 737
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- Anusavice, K. J. 2002. Dental caries: risk assessment and treatment solutions for an elderly population. Compend. Contin. Educ. Dent.23:12-20.
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Occlusal Caries: Biological Approach for Its Diagnosis and Management
Carvalho, J. C., Dige, I., Mačhiulskienė, V., Qvist, V., Bakhshandeh, A., Parolo, C. C. F., & Maltz, M. (2016) · Caries Research · doi:10.1159/000448662 · Cited by 2,159
“Site-specific sampling of occlusal caries lesions has already been performed and analyzed by the newer molecular techniques, but the studies have been limited to only include more advanced stages of caries lesions involving the dentin [Munson et al., 2004; Aas et al., 2008; Arif et al., 2008; Mantzourani et al., 2009;…”
BackgroundMethodInfluentialGlobal Epidemiology of Campylobacter Infection
Kaakoush, N. O., Castaño‐Rodríguez, N., Mitchell, H. M., & Man, S. M. (2015) · Clinical Microbiology Reviews · doi:10.1128/cmr.00006-15 · Cited by 1,542
“showae in plaque from whitespot or dentin lesions of patients with periodontal disease compared with the levels in healthy subjects (135), while another reported that C.”
ResultThe Role of Bacteria in the Caries Process
Takahashi, N., & Nyvad, B. (2010) · Journal of Dental Research · doi:10.1177/0022034510379602 · Cited by 1,250
Exploring Microbial Diversity and Taxonomy Using SSU rRNA Hypervariable Tag Sequencing
Huse, S. M., Dethlefsen, L., Huber, J. A., Welch, D. B. M., Relman, D. A., & Sogin, M. L. (2008) · PLoS Genetics · doi:10.1371/journal.pgen.1000255 · Cited by 1,020
“Other recent human health studies include microbes in breast milk [18], chronic wounds [19], human gut [20], dental caries [21], and childcare facilities [22].”
BackgroundEcological Hypothesis of Dentin and Root Caries
Takahashi, N., & Nyvad, B. (2016) · Caries Research · doi:10.1159/000447309 · Cited by 737
“Proteolytic bacteria, such as Prevotella and Propionibacterium species, have been isolated from dentin carious lesions [Aas et al., 2008] and root surfaces [Preza et al.”
BackgroundInfluential
Metadata
- Authors
- Aas, J. A., Griffen, A. L., Dardis, S. R., Lee, A. M., Olsen, I., Dewhirst, F. E., Leys, E. J., & Paster, B. J.
- Affiliations
- University of Oslo (NO)
- Department of Pediatric Dentistry
- The Forsyth Institute (US)
- Harvard University (US)
- The Ohio State University (US)
- Corresponding author
- Aas, Jørn A.
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-04
- Volume
- 46
- Issue
- 4
- Pages
- 1407-1417
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC2292933
- OpenAlex
- W2104771379
- Cited by
- 952
- References
- 42
- Citations by year
- 2026: 22 · 2025: 36 · 2024: 61 · 2023: 62 · 2022: 87 · 2021: 80 · 2020: 84 · 2019: 64 · 2018: 63 · 2017: 46 · 2016: 50 · 2015: 42 · 2014: 53 · 2013: 43 · 2012: 46
- FWCI
- 42.44
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Universitetet i Oslo
- National Institutes of Health
- National Institute of Dental and Craniofacial Research (U01DE016937, DE016125, R01DE016125, DE11443, R01DE011443)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Dentistry › General Dentistry › Dental Health and Care Utilization
- Health Sciences › Dentistry › General Dentistry › Dental materials and restorations
- Keywords
- dental caries, Streptococcus mutans, primary teeth, permanent teeth, bacterial species composition, 16S rRNA gene sequencing, white spot lesions, dentin lesions, Lactobacillus, Bifidobacterium dentium, Atopobium, Propionibacterium, Actinomyces, Veillonella, caries progression, caries etiology, children and young adults
- MeSH terms
- Adolescent, Adult, Child, Child, Preschool, Dental Caries, Gram-Positive Bacteria, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S, Tooth, Tooth, Deciduous, Gram-Positive Bacterial Infections, Sequence Analysis, DNA
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