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Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA
Open accessAbstract
Human keratinocytes and fibroblasts isolated from foreskin were transformed by transfection with recombinant human papillomavirus type 16 (HPV16) DNA. The transformed cells exhibited an extended (fibroblasts) or indefinite (keratinocytes) life-span compared with that of normal controls. In addition, HS27, a human fibroblast cell line previously transfected with origin-defective simian virus 40, was successfully transfected. HPV16 sequences were stably maintained in the cells, and extensive amplification and rearrangements occurred with continuous culturing. Moreover, both fibroblasts and keratinocytes expressed several specific HPV16 mRNAs. Because HPV16-transfected cells had viral transcripts and because transfection with the vector alone did not extend the life-span of the cells, it is likely that the virus was responsible for the indefinite life-span. Transfected fibroblast and keratinocyte lines will be useful for investigating the molecular biology of HPV16 and the interactions between the viral DNA and the human genome. Moreover, transfected keratinocytes provide a model for analyzing the effects of HPV16 on the differentiation properties of human epithelial cells.
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Citations
Cited by
Papillomaviruses in the causation of human cancers - a brief historical account
zur Hausen, H. (2009) · Virology · doi:10.1016/j.virol.2008.11.046 · Cited by 1,532
Münger, K., Phelps, W. C., Bubb, V, Howley, P. M., & Schlegel, R. (1989) · Journal of Virology · doi:10.1128/jvi.63.10.4417-4421.1989 · Cited by 1,030
Münger, K., Werness, B. A., Dyson, N., Phelps, W. C., Harlow, E., & Howley, P. M. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb08594.x · Cited by 964
HK-2: An immortalized proximal tubule epithelial cell line from normal adult human kidney
Ryan, M. J., Johnson, G., Kirk, J., Fuerstenberg, S. M., Zager, R. A., & Torok-Storb, B. (1994) · Kidney International · doi:10.1038/ki.1994.6 · Cited by 891
Huibregtse, J. M., Scheffner, M., & Howley, P. M. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04990.x · Cited by 879
Metadata
- Authors
- Pirisi, L., Yasumoto, S., Feller, M., Doniger, J., & DiPaolo, J. A.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1987-04
- Volume
- 61
- Issue
- 4
- Pages
- 1061-1066
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1858263251
- Cited by
- 528
- References
- 0
- FWCI
- 37.25
- Type
- journal article
- Language
- en
- Open access
- green
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- Pirisi, L., Yasumoto, S., Feller, M., Doniger, J., & DiPaolo, J. A. (1987). Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA. Journal of Virology. https://doi.org/10.1128/jvi.61.4.1061-1066.1987
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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