The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1
Open accessAbstract
We have used the polymerase chain reaction technique to clone the small multiply spliced mRNA species produced after infection of human cells by a molecular clone of human immunodeficiency virus type 1 (HIV-1). We identified six Rev-expressing mRNAs, which were generated by the use of two splice acceptors located immediately upstream of the rev AUG. The class of small mRNAs included 12 mRNAs expressing Tat, Rev, and Nef. In addition, HIV-1 produced other multiply spliced mRNAs that used alternative splice sites identified by cloning and sequencing. All of these mRNAs were found in the cytoplasm and should be able to produce additional proteins. The coding capacity of the tat, rev, and nef mRNAs was analyzed by transfection of the cloned cDNAs into human cells. The tat mRNAs produced high levels of Tat, but very low levels of Rev and Nef. All the rev mRNAs expressed high levels of both Rev and Nef and were essential for the production of sufficient amounts of Rev. Therefore, HIV-1 uses both alternatively spliced and bicistronic mRNAs for the production of Tat, Rev, and Nef proteins.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
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The pX protein of HTLV-I is a transcriptional activator of its long terminal repeats
Felber, B. K., Paskalis, H., Kleinman-Ewing, C., Wong-Staal, F., & Pavlakis, G. N. (1985) · Science · doi:10.1126/science.2992082 · Cited by 516
Self-citation
Cited by
An analysis of vertebrate mRNA sequences: intimations of translational control.
Kozak, M. (1991) · The Journal of cell biology · doi:10.1083/jcb.115.4.887 · Cited by 1,635
Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes
Huang, J., Wang, F., Argyris, E., Chen, K., Liang, Z., Tian, H., Huang, W., Squires, K., Verlinghieri, G., & Zhang, H. (2007) · Nature Medicine · doi:10.1038/nm1639 · Cited by 768
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Metadata
- Authors
- Schwartz, S., Felber, B. K., Benko, D. M., Fenyö, E. M., & Pavlakis, G. N.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1990-06
- Volume
- 64
- Issue
- 6
- Pages
- 2519-2529
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1840089255
- Cited by
- 423
- References
- 4
- FWCI
- 9.74
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Schwartz, S., Felber, B. K., Benko, D. M., Fenyö, E. M., & Pavlakis, G. N. (1990). Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1. Journal of Virology. https://doi.org/10.1128/jvi.64.6.2519-2529.1990
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
