The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Epstein-Barr virus nuclear antigen 2 transactivates latent membrane protein LMP1
Open accessAbstract
Several lines of evidence are compatible with the hypothesis that Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA-2) or leader protein (EBNA-LP) affects expression of the EBV latent infection membrane protein LMP1. We now demonstrate the following. (i) Acute transfection and expression of EBNA-2 under control of simian virus 40 or Moloney murine leukemia virus promoters resulted in increased LMP1 expression in P3HR-1-infected Burkitt's lymphoma cells and the P3HR-1 or Daudi cell line. (ii) Transfection and expression of EBNA-LP alone had no effect on LMP1 expression and did not act synergistically with EBNA-2 to affect LMP1 expression. (iii) LMP1 expression in Daudi and P3HR-1-infected cells was controlled at the mRNA level, and EBNA-2 expression in Daudi cells increased LMP1 mRNA. (iv) No other EBV genes were required for EBNA-2 transactivation of LMP1 since cotransfection of recombinant EBNA-2 expression vectors and genomic LMP1 DNA fragments enhanced LMP1 expression in the EBV-negative B-lymphoma cell lines BJAB, Louckes, and BL30. (v) An EBNA-2-responsive element was found within the -512 to +40 LMP1 DNA since this DNA linked to a chloramphenicol acetyltransferase reporter gene was transactivated by cotransfection with an EBNA-2 expression vector. (vi) The EBV type 2 EBNA-2 transactivated LMP1 as well as the EBV type 1 EBNA-2. (vii) Two deletions within the EBNA-2 gene which rendered EBV transformation incompetent did not transactivate LMP1, whereas a transformation-competent EBNA-2 deletion mutant did transactivate LMP1. LMP1 is a potent effector of B-lymphocyte activation and can act synergistically with EBNA-2 to induce cellular CD23 gene expression. Thus, EBNA-2 transactivation of LMP1 amplifies the biological impact of EBNA-2 and underscores its central role in EBV-induced growth transformation.
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Cites
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SPECTRUM OF EPSTEIN-BARR VIRUS–ASSOCIATED DISEASES
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Self-citationNasopharyngeal carcinoma—Review of the molecular mechanisms of tumorigenesis
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Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation
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Metadata
- Authors
- Wang, F., Tsang, S. F., Kurilla, M. G., Cohen, J. I., & Kieff, E.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1990-07
- Volume
- 64
- Issue
- 7
- Pages
- 3407-3416
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1812374189
- Cited by
- 303
- References
- 36
- FWCI
- 8.87
- Type
- journal article
- Language
- en
- Open access
- green
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