The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA
Open accessAbstract
Previous reports suggest that the hepatitis C virus (HCV) genome RNA terminates with homopolymer tracts of either poly(U) or poly(A). By ligation of synthetic oligonucleotides followed by reverse transcription-PCR, cDNA cloning, and sequence analysis, we determined the 3'-terminal sequence of HCV genome RNA. Our results show that the HCV 3' nontranslated region consists of four elements (positive sense, 5' to 3'): (i) a short sequence with significant variability among genotypes, (ii) a homopolymeric poly(U) tract, (iii) a polypyrimidine stretch consisting of mainly U with interspersed C residues, (iv) a novel sequence of 98 bases. This latter nucleotide sequence is not present in human genomic DNA and is highly conserved among HCV genotypes. The 3'-terminal 46 bases are predicted to form a stable stem-loop structure. Using a quantitative-competitive reverse transcription-PCR assay, we show that a substantial fraction of HCV genome RNAs from a high- specific-infectivity inoculum contain this 3'-terminal sequence element. These results indicate that the HCV genome RNA terminates with a highly conserved RNA element which is likely to be required for authentic HCV replication and recovery of infectious RNA from cDNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Isolation of a cDNA cLone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis Genome
Choo, Q.-L., Kuo, G., Weiner, A. J., Overby, L. R., Bradley, D. W., & Houghton, M. (1989) · Science · doi:10.1126/science.2523562 · Cited by 6,875
The alphaviruses: gene expression, replication, and evolution.
Strauss, J. H., & Strauss, E. G. (1994) · Microbiological Reviews · doi:10.1128/mmbr.58.3.491-562.1994 · Cited by 2,221
FLAVIVIRUS GENOME ORGANIZATION, EXPRESSION, AND REPLICATION
Chambers, T. J., Hahn, C. S., Galler, R., & Rice, C. M. (1990) · Annual Review of Microbiology · doi:10.1146/annurev.mi.44.100190.003245 · Cited by 2,014
Self-citationKato, N., Hijikata, M., Ootsuyama, Y., Nakagawa, M., Ohkoshi, S., Sugimura, T., & Shimotohno, K. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.24.9524 · Cited by 1,191
Barnes, W. M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.6.2216 · Cited by 1,034
Genetic Heterogeneity of Hepatitis C Virus: Quasispecies and Genotypes
Bukh, J., Miller, R., & Purcell, R. (1995) · Seminars in Liver Disease · doi:10.1055/s-2007-1007262 · Cited by 833
Improved predictions of secondary structures for RNA.
Jaeger, J. A., Turner, D. H., & Zuker, M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.20.7706 · Cited by 817
Wimmer, E., Hellen, C. U. T., & Cao, X. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.002033 · Cited by 554
Identification of two flavivirus-like genomes in the GB hepatitis agent.
Simons, J. N., Pilot-Matias, T. J., Leary, T. P., Dawson, G. J., Desai, S. M., Schlauder, G. G., Muerhoff, A. S., Erker, J. C., Buijk, S. L., & Chalmers, M. L. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.8.3401 · Cited by 551
Singh, R., Valcárcel, J., & Green, M. R. (1995) · Science · doi:10.1126/science.7761834 · Cited by 537
Cited by
The HCV Life Cycle: In vitro Tissue Culture Systems and Therapeutic Targets
Gerold, G., & Pietschmann, T. (2014) · Digestive Diseases · doi:10.1159/000360830 · Cited by 4,854
Consensus Proposals for a Unified System of Nomenclature of Hepatitis C Virus Genotypes *
Simmonds, P., Bukh, J., Combet, C., Deléage, G., Enomoto, N., Feinstone, S., Halfon, P., Inchauspé, G., Kuiken, C., Maertens, G., et al. (2005) · Hepatology · doi:10.1002/hep.20819 · Cited by 1,516
Self-citationKissing-Loop Interaction in the 3′ End of the Hepatitis C Virus Genome Essential for RNA Replication
Friebe, P., Boudet, J., Simorre, J.-P., & Bartenschlager, R. (2005) · Journal of Virology · doi:10.1128/jvi.79.1.380-392.2005 · Cited by 371
Novel Insights into Hepatitis C Virus Replication and Persistence
Bartenschlager, R., Frese, M., & Pietschmann, T. (2004) · Advances in Virus Research · doi:10.1016/s0065-3527(04)63002-8 · Cited by 307
Characterization of a canine homolog of hepatitis C virus
Kapoor, A., Simmonds, P., Gerold, G., Qaisar, N., Jain, K., Henriquez, J. A., Firth, C., Hirschberg, D. L., Rice, C. M., Shields, S., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1101794108 · Cited by 288
Self-citation
Metadata
- Authors
- Kolykhalov, A. A., Feinstone, S. M., & Rice, C. M.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1996-06
- Volume
- 70
- Issue
- 6
- Pages
- 3363-3371
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W1914884504
- Cited by
- 468
- References
- 19
- FWCI
- 20.53
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA. https://app.cochat.ai/papers/10.1128/jvi.70.6.3363-3371.1996
- The paper
- Kolykhalov, A. A., Feinstone, S. M., & Rice, C. M. (1996). Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA. Journal of Virology. https://doi.org/10.1128/jvi.70.6.3363-3371.1996
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
