The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Cloning of the Human Cytomegalovirus (HCMV) Genome as an Infectious Bacterial Artificial Chromosome in Escherichia coli : a New Approach for Construction of HCMV Mutants
Open accessAbstract
We have recently introduced a novel procedure for the construction of herpesvirus mutants that is based on the cloning and mutagenesis of herpesvirus genomes as infectious bacterial artificial chromosomes (BACs) in Escherichia coli (M. Messerle, I. Crnković, W. Hammerschmidt, H. Ziegler, and U. H. Koszinowski, Proc. Natl. Acad. Sci. USA 94:14759–14763, 1997). Here we describe the application of this technique to the human cytomegalovirus (HCMV) strain AD169. Since it was not clear whether the terminal and internal repeat sequences of the HCMV genome would give rise to recombination, the stability of the cloned HCMV genome was examined during propagation in E. coli , during mutagenesis, and after transfection in permissive fibroblasts. Interestingly, the HCMV BACs were frozen in defined conformations in E. coli . The transfection of the HCMV BACs into human fibroblasts resulted in the reconstitution of infectious virus and isomerization of the reconstituted genomes. The power of the BAC mutagenesis procedure was exemplarily demonstrated by the disruption of the gpUL37 open reading frame. The transfection of the mutated BAC led to plaque formation, indicating that the gpUL37 gene product is dispensable for growth of HCMV in fibroblasts. The new procedure will considerably speed up the construction of HCMV mutants and facilitate genetic analysis of HCMV functions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Cold Spring Harbor Laboratory; Cold Spring Harbor, NY: 1979 xxxvi + 1388 pages. $120.00
Cavalier-Smith, T. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80323-1 · Cited by 6,273
Selective extraction of polyoma DNA from infected mouse cell cultures
Hirt, B. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90307-5 · Cited by 5,146
Cherepanov, P. P., & Wackernagel, W. (1995) · Gene · doi:10.1016/0378-1119(95)00193-a · Cited by 1,937
Shizuya, H., Birren, B., Kim, U. J., Mancino, V, Slepak, T., Tachiiri, Y., & Simon, M. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.18.8794 · Cited by 1,671
Analysis of the Protein-Coding Content of the Sequence of Human Cytomegalovirus Strain AD169
Chee, M. S., Bankier, A. T., Beck, S., Bohni, R., Brown, C. M., Cerny, R., Horsnell, T., Hutchison, C. A., Kouzarides, T., Martignetti, J. A., et al. (1990) · Current Topics in Microbiology and Immunology · doi:10.1007/978-3-642-74980-3_6 · Cited by 1,252
Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains
Cha, T. A., Tom, E., Kemble, G. W., Duke, G. M., Mocarski, E. S., & Spaete, R. R. (1996) · Journal of Virology · doi:10.1128/jvi.70.1.78-83.1996 · Cited by 611
Propagation and recovery of intact, infectious Epstein–Barr virus from prokaryotic to human cells
Delecluse, H.-J., Hilsendegen, T., Pich, D., Zeidler, R., & Hammerschmidt, W. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.14.8245 · Cited by 467
Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome
Messerle, M., Crnkovic, I., Hammerschmidt, W., Ziegler, H., & Koszinowski, U. H. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.26.14759 · Cited by 426
Self-citationWagner, M., Jonjić, S., Koszinowski, U. H., & Messerle, M. (1999) · Journal of Virology · doi:10.1128/jvi.73.8.7056-7060.1999 · Cited by 320
Self-citation
Cited by
Coding potential of laboratory and clinical strains of human cytomegalovirus
Murphy, E., Yu, D., Grimwood, J., Schmutz, J., Dickson, M., Jarvis, M. A., Hahn, G., Nelson, J. A., Myers, R. M., & Shenk, T. E. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2136652100 · Cited by 498
Self-citationPeptide-specific recognition of human cytomegalovirus strains controls adaptive natural killer cells
Hammer, Q., Rückert, T., Borst, E. M., Dunst, J., Haubner, A., Durek, P., Heinrich, F., Gasparoni, G., Babic, M., Tomic, A., et al. (2018) · Nature Immunology · doi:10.1038/s41590-018-0082-6 · Cited by 423
Self-citationHuman Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic Genes
Marchini, A., Liu, H., & Zhu, H. (2001) · Journal of Virology · doi:10.1128/jvi.75.4.1870-1878.2001 · Cited by 332
Adler, H., Messerle, M., Wagner, M., & Koszinowski, U. H. (2000) · Journal of Virology · doi:10.1128/jvi.74.15.6964-6974.2000 · Cited by 317
Self-citationGrey, F., Meyers, H., White, E. A., Spector, D. H., & Nelson, J. (2007) · PLoS Pathogens · doi:10.1371/journal.ppat.0030163 · Cited by 263
Metadata
- Authors
- Borst, E.-M., Hahn, G., Koszinowski, U. H., & Messerle, M.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1999-10
- Volume
- 73
- Issue
- 10
- Pages
- 8320-8329
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2141755034
- Cited by
- 384
- References
- 29
- FWCI
- 11.48
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cloning of the Human Cytomegalovirus (HCMV) Genome as an Infectious Bacterial Artificial Chromosome in Escherichia coli : a New Approach for Construction of HCMV Mutants. https://app.cochat.ai/papers/10.1128/jvi.73.10.8320-8329.1999
- The paper
- Borst, E.-M., Hahn, G., Koszinowski, U. H., & Messerle, M. (1999). Cloning of the Human Cytomegalovirus (HCMV) Genome as an Infectious Bacterial Artificial Chromosome in Escherichia coli : a New Approach for Construction of HCMV Mutants. Journal of Virology. https://doi.org/10.1128/jvi.73.10.8320-8329.1999
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
