The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Human Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic Genes
Open accessAbstract
Much evidence suggests that the major immediate-early (IE) transactivator of human cytomegalovirus (HCMV), IE-2, is likely to be critical for efficient viral replication; however, the lack of an IE-2 mutant HCMV has precluded an experimental test of this hypothesis. As an initial step toward characterizing an IE-2 mutant, we first cloned the HCMV Towne genome as a bacterial artificial chromosome (BAC) and analyzed the ability of transfected Towne-BAC DNA (T-BACwt) to produce plaques following introduction into permissive human fibroblasts. Like Towne viral DNA, transfected T-BACwt DNA was infectious in permissive cells, and the resulting virus stocks were indistinguishable from Towne virus. We then used homologous recombination in Escherichia coli to delete the majority of UL122, the open reading frame encoding the unique portion of IE-2, from T-BACwt. From this deleted BAC, a third BAC clone in which the deletion was repaired with wild-type UL122 was created. In numerous transfections of permissive human foreskin fibroblast cells with these three BAC DNA clones, the rescued BAC and T-BACwt consistently yielded plaques, while the UL122 mutant BAC never generated plaques, even after 4 weeks. Protein and mRNA of other IE genes were readily detected from transfected UL122 mutant BAC DNA; however, reverse transcription-PCR failed to detect mRNA expression from any of five early genes examined. The generalized failure of this mutant to express early genes is consistent with expectations from in vitro assays which have demonstrated that IE-2 transactivates most HCMV promoters. These experiments provide the first direct demonstration that IE-2 is required for successful HCMV infection and indicate that virus lacking IE-2 arrests early in the replication cycle.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Analysis of the Protein-Coding Content of the Sequence of Human Cytomegalovirus Strain AD169
Chee, M. S., Bankier, A. T., Beck, S., Bohni, R., Brown, C. M., Cerny, R., Horsnell, T., Hutchison, C. A., Kouzarides, T., Martignetti, J. A., et al. (1990) · Current Topics in Microbiology and Immunology · doi:10.1007/978-3-642-74980-3_6 · Cited by 1,252
Retinoblastoma protein recruits histone deacetylase to repress transcription
Brehm, A., Miska, E. A., McCance, D. J., Reid, J. L., Bannister, A. J., & Kouzarides, T. (1998) · Nature · doi:10.1038/35404 · Cited by 1,230
de Lorenzo, V., & Timmis, K. N. (1994) · Methods in Enzymology · doi:10.1016/0076-6879(94)35157-0 · Cited by 879
Potential Role of Human Cytomegalovirus and p53 Interaction in Coronary Restenosis
Speir, E., Modali, R., Huang, E.-S., Leon, M. B., Shawl, F., Finkel, T., & Epstein, S. E. (1994) · Science · doi:10.1126/science.8023160 · Cited by 753
Fowler, K. B., McCollister, F. P., Dahle, A. J., Boppana, S., Britt, W. J., & Pass, R. F. (1997) · The Journal of Pediatrics · doi:10.1016/s0022-3476(97)70248-8 · Cited by 549
Human cytomegalovirus IE1 and IE2 proteins block apoptosis
Zhu, H., Shen, Y., & Shenk, T. (1995) · Journal of Virology · doi:10.1128/jvi.69.12.7960-7970.1995 · Cited by 468
Self-citationLongitudinal Investigation of Hearing Disorders in Children with Congenital Cytomegalovirus
Dahle, A. J., Fowler, K. B., Wright, J. D., Boppana, S. B., Britt, W. J., & Pass, R. F. (2000) · Journal of the American Academy of Audiology · doi:10.1055/s-0042-1748054 · Cited by 442
Borst, E.-M., Hahn, G., Koszinowski, U. H., & Messerle, M. (1999) · Journal of Virology · doi:10.1128/jvi.73.10.8320-8329.1999 · Cited by 384
Zhang, H. S., Postigo, A. A., & Dean, D. C. (1999) · Cell · doi:10.1016/s0092-8674(00)80714-x · Cited by 349
Wathen, M. W., & Stinski, M. F. (1982) · Journal of Virology · doi:10.1128/jvi.41.2.462-477.1982 · Cited by 305
Cited by
The Antiviral Activities of Artemisinin and Artesunate
Efferth, T., Romero, M. R., Wolf, D. G., Stamminger, T., Marin, J. J. G., & Marschall, M. (2008) · Clinical Infectious Diseases · doi:10.1086/591195 · Cited by 503
Coding potential of laboratory and clinical strains of human cytomegalovirus
Murphy, E., Yu, D., Grimwood, J., Schmutz, J., Dickson, M., Jarvis, M. A., Hahn, G., Nelson, J. A., Myers, R. M., & Shenk, T. E. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2136652100 · Cited by 498
Landolfo, S., Gariglio, M., Gribaudo, G., & Lembo, D. (2003) · Pharmacology & Therapeutics · doi:10.1016/s0163-7258(03)00034-2 · Cited by 358
Stanton, R. J., Baluchova, K., Dargan, D. J., Cunningham, C., Sheehy, O., Seirafian, S., McSharry, B. P., Neale, M. L., Davies, J. A., Tomasec, P., et al. (2010) · Journal of Clinical Investigation · doi:10.1172/jci42955 · Cited by 256
Zhen, H., Fang, F., Ye, D.-Y., Shu, S.-N., Zhou, Y.-F., Dong, Y.-S., Nie, X.-C., & Li, G. (2006) · Antiviral Research · doi:10.1016/j.antiviral.2006.03.017 · Cited by 73
Metadata
- Authors
- Marchini, A., Liu, H., & Zhu, H.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2001-02-15
- Volume
- 75
- Issue
- 4
- Pages
- 1870-1878
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2117505476
- Cited by
- 332
- References
- 26
- FWCI
- 13.79
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Human Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic Genes. https://app.cochat.ai/papers/10.1128/jvi.75.4.1870-1878.2001
- The paper
- Marchini, A., Liu, H., & Zhu, H. (2001). Human Cytomegalovirus with IE-2 (UL122) Deleted Fails To Express Early Lytic Genes. Journal of Virology. https://doi.org/10.1128/jvi.75.4.1870-1878.2001
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
