The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Human Adeno-Associated Virus Type 5 Is Only Distantly Related to Other Known Primate Helper-Dependent Parvoviruses
Open accessAbstract
We have characterized 95% (4,404 nucleotides) of the genome of adeno-associated virus type 5 (AAV5), including part of the terminal repeats and the terminal resolution site. Our results show that AAV5 is different from all other described AAV serotypes at the nucleotide level and at the amino acid level. The sequence homology to AAV2, AAV3B, AAV4, and AAV6 at the nucleotide level is only between 54 and 56%. The positive strand contains two large open reading frames (ORFs). The left ORF encodes the nonstructural (Rep) proteins, and the right ORF encodes the structural (Cap) proteins. At the amino acid level the identities with the capsid proteins of other AAVs range between 51 and 59%, with a high degree of heterogeneity in regions which are considered to be on the exterior surface of the viral capsid. The overall identity for the nonstructural Rep proteins at the amino acid level is 54.4%. It is lowest at the C-terminal 128 amino acids (10%). There are only two instead of the common three putative Zn fingers in the Rep proteins. The Cap protein data suggest differences in capsid surfaces and raise the possibility of a host range distinct from those of other parvoviruses. This may have important implications for AAV vectors used in gene therapy.
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“AAV5 has the most distinct sequence of the capsids (AAV5: 61.2% amino acid sequence identity to AAV2, AAV8: 83.6% amino acid sequence identity to AAV2, AAV9: 82.7% sequence identity to AAV2),55,56”
From Antibody neutralization poses a barrier to intravitreal adeno-associated viral vector gene delivery to non-human primates (2015) · cites this paper as [56]
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Metadata
- Authors
- Bantel-Schaal, U., Delius, H., Schmidt, R., & zur Hausen, H.
- Affiliations
- <!--label omitted: 1-->Deutsches Krebsforschungszentrum Heidelberg, Forschungsschwerpunkt Angewandte Tumorvirologie F0400, D-69120 Heidelberg, Germany
- German Cancer Research Center (DE)
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1999-02
- Volume
- 73
- Issue
- 2
- Pages
- 939-947
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- PMC
- PMC103913
- OpenAlex
- W2119056879
- Cited by
- 128
- References
- 61
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 5 · 2023: 3 · 2022: 6 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 1
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Virology (publishedVersion)
- PubMed Central (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- AAV serotypes, sequence homology, capsid protein, Rep protein, zinc finger, terminal repeats, host range, gene therapy vectors, parvovirus
- MeSH terms
- Dependovirus, Amino Acid Sequence, Animals, Base Sequence, Capsid, DNA-Binding Proteins, DNA, Viral, HeLa Cells, Helper Viruses, Humans, Molecular Sequence Data, Primates, Viral Proteins, Genome, Viral, Sequence Homology, Amino Acid, Parvovirus
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