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Cited by 128Virus-based gene therapy researchParvovirus B19 Infection StudiesVirology and Viral Diseases

The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.

Human Adeno-Associated Virus Type 5 Is Only Distantly Related to Other Known Primate Helper-Dependent Parvoviruses

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Bantel-Schaal, Ursula, Delius, Hajo, Schmidt, Rainer, zur Hausen, Harald·1999·Journal of Virology
DOI 10.1128/jvi.73.2.939-947.1999PMC PMC103913
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Abstract

We have characterized 95% (4,404 nucleotides) of the genome of adeno-associated virus type 5 (AAV5), including part of the terminal repeats and the terminal resolution site. Our results show that AAV5 is different from all other described AAV serotypes at the nucleotide level and at the amino acid level. The sequence homology to AAV2, AAV3B, AAV4, and AAV6 at the nucleotide level is only between 54 and 56%. The positive strand contains two large open reading frames (ORFs). The left ORF encodes the nonstructural (Rep) proteins, and the right ORF encodes the structural (Cap) proteins. At the amino acid level the identities with the capsid proteins of other AAVs range between 51 and 59%, with a high degree of heterogeneity in regions which are considered to be on the exterior surface of the viral capsid. The overall identity for the nonstructural Rep proteins at the amino acid level is 54.4%. It is lowest at the C-terminal 128 amino acids (10%). There are only two instead of the common three putative Zn fingers in the Rep proteins. The Cap protein data suggest differences in capsid surfaces and raise the possibility of a host range distinct from those of other parvoviruses. This may have important implications for AAV vectors used in gene therapy.

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Research works printed but not linked: 2

  1. Berns K. I. Parvoviridae: the viruses and their replication Fundamental virology 3rd ed. Fields B. N. Knipe D. M. Howley P. M. 1995 1017 1041 Lippincott-Raven Publishers Philadelphia Pa
  2. Carter B. J. Trempe J. P. Mendelson E. Adeno-associated virus gene expression and regulation Handbook of parvoviruses Tijsen P. 1 1990 227 254 CRC Press Boca Raton, Fla

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Metadata

Authors
Bantel-Schaal, U., Delius, H., Schmidt, R., & zur Hausen, H.
Affiliations
  • <!--label omitted: 1-->Deutsches Krebsforschungszentrum Heidelberg, Forschungsschwerpunkt Angewandte Tumorvirologie F0400, D-69120 Heidelberg, Germany
  • German Cancer Research Center (DE)
Venue
Journal of Virology
Publisher
American Society for Microbiology
Published
1999-02
Volume
73
Issue
2
Pages
939-947
ISSN
  • 0022-538X (ISSN-L)
  • 1098-5514
DOI
10.1128/jvi.73.2.939-947.1999
PMC
PMC103913
OpenAlex
W2119056879
Cited by
128
References
61
Citations by year
2026: 2 · 2025: 7 · 2024: 5 · 2023: 3 · 2022: 6 · 2020: 4 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 1
FWCI
5.28
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Journal of Virology (publishedVersion)
  • PubMed Central (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
AAV serotypes, sequence homology, capsid protein, Rep protein, zinc finger, terminal repeats, host range, gene therapy vectors, parvovirus
MeSH terms
Dependovirus, Amino Acid Sequence, Animals, Base Sequence, Capsid, DNA-Binding Proteins, DNA, Viral, HeLa Cells, Helper Viruses, Humans, Molecular Sequence Data, Primates, Viral Proteins, Genome, Viral, Sequence Homology, Amino Acid, Parvovirus

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