Posttranscriptional Regulation of Cell-Cell Interaction Protein-Encoding Transcripts by Zfs1p in Schizosaccharomyces pombe
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Members of the tristetraprolin (TTP) family of CCCH tandem zinc finger proteins can bind directly to AU-rich elements in mRNAs and promote transcript deadenylation and decay. The yeast Schizosaccharomyces pombe expresses a single TTP family member, Zfs1p. In this study, we identified probable Zfs1p target mRNAs by comparing transcript levels in wild-type yeast and zfs1Δ mutants, using deep sequencing and microarray approaches. We also used direct RNA sequencing to determine polyadenylation site locations and to confirm the presence of potential Zfs1p target sequences within the target mRNA. These studies identified a set of transcripts containing potential Zfs1p binding sites that accumulated significantly in the zfs1Δ mutants; a subset of these transcripts decayed more slowly in the zfs1Δ mutants and bound directly to Zfs1p in coimmunoprecipitation assays. One apparent direct target encodes the transcription factor Cbf12p, which is known to increase cell-cell adhesion and flocculation when overexpressed. Studies of zfs1Δ cbf12Δ double mutants demonstrated that the increased flocculation seen in zfs1Δ mutants is due, at least in part, to a direct effect on the turnover of cbf12 mRNA. These data suggest that Zfs1p can both directly and indirectly regulate the levels of transcripts involved in cell-cell adhesion in this species.
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The only other fungus with a functionally described Cth2 ortholog, S. pombe, displays no transcriptional alterations of its iron homeostasis genes when this gene is deleted.
“The only other fungus with a functionally described Cth2 ortholog, Schizosaccharomyces pombe, also displays no transcriptional alterations of its iron homeostasis genes when this gene is deleted (46, 47).”
From A novel hybrid iron regulation network combines features from pathogenic and nonpathogenic yeasts (2016) · cites this paper as [47]
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A novel hybrid iron regulation network combines features from pathogenic and nonpathogenic yeasts
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- Authors
- Wells, M. L., Huang, W., Li, L., Gerrish, K. E., Fargo, D. C., Ozsolak, F., & Blackshear, P. J.
- Affiliations
- National Institutes of Health (US)
- Helicos Biosciences (United States) (US)
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2012-10-01
- Volume
- 32
- Issue
- 20
- Pages
- 4206-4214
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC3457339
- OpenAlex
- W2127992808
- Cited by
- 24
- References
- 40
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Environmental Health Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- tristetraprolin, CCCH zinc finger proteins, AU-rich elements, post-transcriptional regulation, mRNA degradation, polyadenylation sites, cell-cell adhesion, flocculation, Schizosaccharomyces pombe, mRNA turnover
- MeSH terms
- Base Sequence, Binding Sites, Cell Adhesion, Cell Communication, Molecular Sequence Data, Mutation, Nuclear Proteins, RNA, Messenger, Schizosaccharomyces, Transcription Factors, Transcription, Genetic, RNA Stability, Polyadenylation, Schizosaccharomyces pombe Proteins
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