SWI/SNF and Asf1p Cooperate To Displace Histones during Induction of the Saccharomyces cerevisiae HO Promoter
Open accessAbstract
Regulation of the Saccharomyces cerevisiae HO promoter has been shown to require the recruitment of chromatin-modifying and -remodeling enzymes. Despite this, relatively little is known about what changes to chromatin structure occur during the course of regulation at HO. Here, we used indirect end labeling in synchronized cultures to show that the chromatin structure is disrupted in a region that spans bp -600 to -1800 relative to the transcriptional start site. Across this region, there is a loss of canonical nucleosomes and a reduction in histone DNA cross-linking, as monitored by chromatin immunoprecipitation. The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not. This suggests that the SWI/SNF complex is directly involved in nucleosome removal at HO. We also present evidence indicating that the histone chaperone Asf1 assists in this. These observations suggest that SWI/SNF-related complexes in concert with histone chaperones act to remove histone octamers from DNA during the course of gene regulation.
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SWI/SNF modulates nucleosome position to regulate the expression of a subset of genes including stress-responsive genes.
“SWI/SNF modulates nucleosome position to regulate the expression of a subset of genes including stress-responsive genes20,37–40.”
From SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genes (2022) · cites this paper as [40]
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- Authors
- Gkikopoulos, T., Havas, K. M., Dewar, H., & Owen-Hughes, T.
- Affiliations
- University of Dundee (GB)
- Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
- ORCID
- Havas, Kristina M.
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2009-08-01
- Volume
- 29
- Issue
- 15
- Pages
- 4057-4066
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC2715812
- OpenAlex
- W2149366614
- Cited by
- 33
- References
- 62
- Citations by year
- 2024: 1 · 2022: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 2
- FWCI
- 2.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (publishedVersion)
- Funders
- Wellcome Trust (064414)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- SWI/SNF complex, histone displacement, Saccharomyces cerevisiae, chromatin remodeling, histone acetylation, transcriptional regulation, histone octamer
- MeSH terms
- Chromatin, Chromosomal Proteins, Non-Histone, Histones, Mutation, Nucleosomes, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Transcription Factors, Deoxyribonucleases, Type II Site-Specific, Gene Expression Regulation, Fungal, Cell Cycle Proteins, Molecular Chaperones, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae Proteins, Chromatin Assembly and Disassembly, Chromatin Immunoprecipitation
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Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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