Cited by 33Genomics and Chromatin DynamicsChromatin Remodeling and CancerNuclear Structure and Function

SWI/SNF and Asf1p Cooperate To Displace Histones during Induction of the Saccharomyces cerevisiae HO Promoter

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Gkikopoulos, Triantafyllos, Havas, Kristina M., Dewar, Hilary, Owen-Hughes, Tom·2009·Molecular and Cellular Biology

Abstract

Regulation of the Saccharomyces cerevisiae HO promoter has been shown to require the recruitment of chromatin-modifying and -remodeling enzymes. Despite this, relatively little is known about what changes to chromatin structure occur during the course of regulation at HO. Here, we used indirect end labeling in synchronized cultures to show that the chromatin structure is disrupted in a region that spans bp -600 to -1800 relative to the transcriptional start site. Across this region, there is a loss of canonical nucleosomes and a reduction in histone DNA cross-linking, as monitored by chromatin immunoprecipitation. The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not. This suggests that the SWI/SNF complex is directly involved in nucleosome removal at HO. We also present evidence indicating that the histone chaperone Asf1 assists in this. These observations suggest that SWI/SNF-related complexes in concert with histone chaperones act to remove histone octamers from DNA during the course of gene regulation.

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Crossref · 10.1128/mcb.00400-09

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Authors
Gkikopoulos, T., Havas, K. M., Dewar, H., & Owen-Hughes, T.
Affiliations
  • University of Dundee (GB)
  • Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
Venue
Molecular and Cellular Biology
Published
2009-08-01
Volume
29
Issue
15
Pages
4057-4066
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2149366614
Cited by
33
References
62
Citations by year
2024: 1 · 2022: 1 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 3 · 2012: 2
FWCI
2.15
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Discovery Research Portal (University of Dundee) (publishedVersion)
Funders
Wellcome Trust (064414)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
SWI/SNF complex, histone displacement, Saccharomyces cerevisiae, chromatin remodeling, histone acetylation, transcriptional regulation, histone octamer
MeSH terms
Chromatin, Chromosomal Proteins, Non-Histone, Histones, Mutation, Nucleosomes, Promoter Regions, Genetic, Protein Binding, RNA, Messenger, Transcription Factors, Deoxyribonucleases, Type II Site-Specific, Gene Expression Regulation, Fungal, Cell Cycle Proteins, Molecular Chaperones, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae Proteins, Chromatin Assembly and Disassembly, Chromatin Immunoprecipitation

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