Cited by 158Chromatin Remodeling and CancerGenomics and Chromatin DynamicsNuclear Structure and Function

The Swi/Snf Complex Is Important for Histone Eviction during Transcriptional Activation and RNA Polymerase II Elongation In Vivo

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Schwabish, Marc A., Struhl, Kevin·2007·Molecular and Cellular Biology

Abstract

The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.

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Crossref · 10.1128/mcb.00717-07

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  • Tissue-specific gene expression can be dictated by tissue-specific transcription factor binding and by the tissue-specific recruitment of nucleosome-remodeling complexes to transcription factor occupied loci.

    “These data, taken together with our expression analyses, indicate that tissue-specific gene expression can be dictated by tissue-specific transcription factor binding and by the tissue-specific recruitment of nucleosome-remodeling complexes (e.g., SWI/SNF) (Hogan et al. 2006;Schwabish and Struhl 2007) to transcription factor occupied loci.”

    From Locus co-occupancy, nucleosome positioning, and H3K4me1 regulate the functionality of FOXA2-, HNF4A-, and PDX1-bound loci in islets and liver (2010) · cites this paper as Schwabish and Struhl 2007

    The paper’s Results and Discussion (page not provided) show that Swi/Snf is recruited to the SUC2 promoter during activation and is necessary for activator-mediated histone eviction there. This supports the proposed role of a remodeling complex at an activated locus, but the paper does not examine tissue-specific transcription-factor binding or tissue-specific recruitment and gene expression. (LLM, conf=0.88)

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1 of the 64 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. [24]Joshi, A. A., and K. Struhl. 2005. Interaction of the Eaf3 chromodomain with methylated histone H3-K36 mediates preferential histone deacetylation at mRNA coding regions. Mol. Cell 20:971-978. [DOI] [PubMed] [Google Scholar]
  2. [59]Workman, J. L. 2006. Nucleosome displacement in transcription. Genes Dev. 20:2009-2017. [DOI] [PubMed] [Google Scholar]

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Authors
Schwabish, M. A., & Struhl, K.
Affiliations
Harvard University (US)
Corresponding author
Struhl, Kevin
Venue
Molecular and Cellular Biology
Published
2007-10-01
Volume
27
Issue
20
Pages
6987-6995
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2166961829
Cited by
158
References
64
Citations by year
2026: 3 · 2025: 2 · 2024: 7 · 2023: 3 · 2022: 7 · 2021: 11 · 2020: 7 · 2019: 5 · 2018: 8 · 2017: 7 · 2016: 1 · 2015: 7 · 2014: 9 · 2013: 13 · 2012: 15
FWCI
5.04
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (GM30186)
  • National Institute of General Medical Sciences (GM30186, R01GM030186, R37GM030186)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
SWI/SNF complex, histone eviction, nucleosome remodeling, RNA polymerase II elongation, transcriptional activation, FACT complex, internal initiation, enhancers, chromatin structure, preinitiation complex
MeSH terms
Adenosine Triphosphatases, DNA-Binding Proteins, Enhancer Elements, Genetic, Histones, Humans, Peptide Chain Elongation, Translational, Peptide Elongation Factors, Promoter Regions, Genetic, RNA Polymerase II, Saccharomyces cerevisiae, Transcription Factors, Transcriptional Activation, Protein Subunits, Saccharomyces cerevisiae Proteins, beta-Fructofuranosidase, Macromolecular Substances

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