The Swi/Snf Complex Is Important for Histone Eviction during Transcriptional Activation and RNA Polymerase II Elongation In Vivo
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The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.
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Tissue-specific gene expression can be dictated by tissue-specific transcription factor binding and by the tissue-specific recruitment of nucleosome-remodeling complexes to transcription factor occupied loci.
“These data, taken together with our expression analyses, indicate that tissue-specific gene expression can be dictated by tissue-specific transcription factor binding and by the tissue-specific recruitment of nucleosome-remodeling complexes (e.g., SWI/SNF) (Hogan et al. 2006;Schwabish and Struhl 2007) to transcription factor occupied loci.”
From Locus co-occupancy, nucleosome positioning, and H3K4me1 regulate the functionality of FOXA2-, HNF4A-, and PDX1-bound loci in islets and liver (2010) · cites this paper as Schwabish and Struhl 2007
The paper’s Results and Discussion (page not provided) show that Swi/Snf is recruited to the SUC2 promoter during activation and is necessary for activator-mediated histone eviction there. This supports the proposed role of a remodeling complex at an activated locus, but the paper does not examine tissue-specific transcription-factor binding or tissue-specific recruitment and gene expression. (LLM, conf=0.88)
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- Authors
- Schwabish, M. A., & Struhl, K.
- Affiliations
- Harvard University (US)
- Corresponding author
- Struhl, Kevin
- ORCID
- Struhl, Kevin
- Venue
- Molecular and Cellular Biology
- Publisher
- Informa UK Limited
- Published
- 2007-10-01
- Volume
- 27
- Issue
- 20
- Pages
- 6987-6995
- ISSN
- 0270-7306 (ISSN-L)
- 1098-5549
- PMC
- PMC2168902
- OpenAlex
- W2166961829
- Cited by
- 158
- References
- 64
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 7 · 2023: 3 · 2022: 7 · 2021: 11 · 2020: 7 · 2019: 5 · 2018: 8 · 2017: 7 · 2016: 1 · 2015: 7 · 2014: 9 · 2013: 13 · 2012: 15
- FWCI
- 5.04
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular and Cellular Biology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (GM30186)
- National Institute of General Medical Sciences (GM30186, R01GM030186, R37GM030186)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- SWI/SNF complex, histone eviction, nucleosome remodeling, RNA polymerase II elongation, transcriptional activation, FACT complex, internal initiation, enhancers, chromatin structure, preinitiation complex
- MeSH terms
- Adenosine Triphosphatases, DNA-Binding Proteins, Enhancer Elements, Genetic, Histones, Humans, Peptide Chain Elongation, Translational, Peptide Elongation Factors, Promoter Regions, Genetic, RNA Polymerase II, Saccharomyces cerevisiae, Transcription Factors, Transcriptional Activation, Protein Subunits, Saccharomyces cerevisiae Proteins, beta-Fructofuranosidase, Macromolecular Substances
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