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WASH Knockout T Cells Demonstrate Defective Receptor Trafficking, Proliferation, and Effector Function

Piotrowski, Joshua T., Gomez, Timothy S., Schoon, Renee A., Mangalam, Ashutosh K., Billadeau, Daniel D.·2013·Molecular and Cellular Biology

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Crossref · 10.1128/mcb.01288-12

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  • WASH is recruited to endosomes where it is necessary for branched F-actin deposition, a process essential to maintain endolysosomal network structure, promote fission of membrane tubules, and traffic cargo proteins.

    “WASH is recruited to endosomes where it is necessary for branched F-actin deposition, a process that is essential to maintain the structure of the endolysosomal network, to promote effective fission of membrane tubules and trafficking of cargo proteins37,39,41,43,44.”

    From Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling (2019) · cites this paper as [43]

    The Introduction says WASH localizes to endomembranes and participates in trafficking through localized Arp2/3-dependent F-actin nucleation. The Results show collapsed endosomal and lysosomal compartments and defective cargo trafficking in WASH-deficient T cells. Our copy does not establish that this actin process promotes fission of membrane tubules; the References list an earlier study on endosome fission, but that title alone does not support the more specific claim. (LLM, conf=0.85)

    Experimental — accuracy not yet measured
  • Regulation of branched F-actin formation by WASH is needed for proper morphology of the endolysosomal system and endosomal trafficking of a broad range of cargoes.

    “Regulation of branched F-actin formation by WASH is needed for proper morphology of the endolysosomal system and endosomal trafficking of a broad range of cargoes37,39,41–44”

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Metadata

Authors
Piotrowski, J. T., Gomez, T. S., Schoon, R. A., Mangalam, A. K., & Billadeau, D. D.
Venue
Molecular and Cellular Biology
Published
2013-03-01
Volume
33
Issue
5
Pages
958-973
ISSN
1098-5549
Cited by
101
References
4
Language
en

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