CoChat
Sign Up
Cited by 238

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Recruitment of the endosomal WASH complex is mediated by the extended ‘tail’ of Fam21 binding to the retromer protein Vps35

Harbour, Michael E., Breusegem, Sophia Y., Seaman, Matthew N. J.·2012·Biochemical Journal
DOI 10.1042/bj20111761
Open this paper in CoChat — free account

Abstract

The retromer complex is a conserved endosomal protein sorting complex that sorts membrane proteins into nascent endosomal tubules. The recognition of membrane proteins is mediated by the cargo-selective retromer complex, a stable trimer of the Vps35 (vacuolar protein sorting 35), Vps29 and Vps26 proteins. We have recently reported that the cargo-selective retromer complex associates with the WASH (Wiskott–Aldrich syndrome homologue) complex, a multimeric protein complex that regulates tubule dynamics at endosomes. In the present study, we show that the retromer–WASH complex interaction occurs through the long unstructured ‘tail’ domain of the WASH complex–Fam21 protein binding to Vps35, an interaction that is necessary and sufficient to target the WASH complex to endosomes. The Fam21-tail also binds to FKBP15 (FK506-binding protein 15), a protein associated with ulcerative colitis, to mediate the membrane association of FKBP15. Elevated Fam21-tail expression inhibits the association of the WASH complex with retromer, resulting in increased cytoplasmic WASH complex. Additionally, overexpression of the Fam21-tail results in cell-spreading defects, implicating the activity of the WASH complex in regulating the mobilization of membrane into the endosome-to-cell surface pathway.

Overview

Integrity signals

Registry

Crossref · 10.1042/bj20111761

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • SNX27 regulates cargo recycling by engaging with the Retromer complex, a SNX-BAR dimer, and the WASH complex.

    “SNX27 regulates cargo recycling by engaging with the Retromer complex (VPS35, VPS26A/B, and VPS29), a SNX–Bin/Amphiphysin/Rvs (BAR) dimer (SNX1/2 in complex with SNX5/6), and the WASH complex”

    From PI(3)P coordinates SNX17- and SNX27-dependent protein recycling for long-term synaptic plasticity (2025) · cites this paper as Harbour et al., 2012

  • VPS35 binds to multiple LFa repeats in FAM21 tail and this interaction is suggested to be sufficient and essential to recruit WASH complex to endosomes.

    “The retromer subunit VPS35 binds to multiple LFa repeats in the FAM21 tail and this interaction is suggested to be sufficient and essential to recruit the WASH complex to endosomes20, 21.”

    From Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling (2017) · cites this paper as [20]

  • FAM21 and WASH complex are recruited to endosomes through retromer-dependent and retromer-independent mechanisms.

    “FAM21 and the WASH complex are therefore recruited to endosomes through retromer-dependent20, 21 and retromer-independent mechanism(s).”

    From Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling (2017) · cites this paper as [20]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 44

  1. Jovic, 2010, The early endosome: a busy sorting station for proteins at the crossroads, Histol. Histopathol., 25, 99
  2. Balasubramanian, 2007, Arf6 and microtubules in adhesion-dependent trafficking of lipid rafts, Nat. Cell Biol., 9, 1381, 10.1038/ncb1657 · DOI
  3. Jović, 2007, EHD1 regulates β1 integrin endosomal transport: effects on focal adhesions, cell spreading and migration, J. Cell Sci., 120, 802, 10.1242/jcs.03383 · DOI
  4. Veale, 2011, VAMP3 regulates podosome organisation in macrophages and together with Stx4/SNAP23 mediates adhesion, cell spreading and persistent migration, Exp. Cell Res., 317, 1817, 10.1016/j.yexcr.2011.04.016 · DOI
  5. Rosenbaum, 2011, Niemann–Pick type C disease: molecular mechanisms and potential therapeutic approaches, J. Neurochem., 116, 789, 10.1111/j.1471-4159.2010.06976.x · DOI
  6. Salinas, 2008, Hereditary spastic paraplegia: clinical features and pathogenetic mechanisms, Lancet Neurol., 7, 1127, 10.1016/S1474-4422(08)70258-8 · DOI · Paper page
  7. Blackstone, 2011, Hereditary spastic paraplegias: membrane traffic and the motor pathway, Nat. Rev. Neurosci., 12, 31, 10.1038/nrn2946 · DOI
  8. Burd, 2011, Physiology and pathology of endosome-to-Golgi retrograde sorting, Traffic, 12, 948, 10.1111/j.1600-0854.2011.01188.x · DOI
  9. Toei, 2010, Regulation and isoform function of the V-ATPases, Biochemistry, 49, 4715, 10.1021/bi100397s · DOI
  10. Marshansky, 2008, The V-type H+-ATPase in vesicular trafficking: targeting, regulation and function, Curr. Opin. Cell Biol., 20, 415, 10.1016/j.ceb.2008.03.015 · DOI
  11. Arighi, 2004, Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor, J. Cell Biol., 165, 123, 10.1083/jcb.200312055 · DOI · Paper page
  12. Seaman, 2004, Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer, J. Cell Biol., 165, 111, 10.1083/jcb.200312034 · DOI · Paper page
  13. Canuel, 2008, AP-1 and retromer play opposite roles in the trafficking of sortilin between the Golgi apparatus and the lysosomes, Biochem. Biophys. Res. Commun., 366, 724, 10.1016/j.bbrc.2007.12.015 · DOI
  14. Nielsen, 2007, Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA, Mol. Cell Biol., 27, 6842, 10.1128/MCB.00815-07 · DOI · Paper page
  15. Tabuchi, 2010, Retromer-mediated direct sorting is required for proper endosomal recycling of the mammalian iron transporter DMT1, J. Cell Sci., 123, 756, 10.1242/jcs.060574 · DOI
  16. Seaman, 2005, Recycle your receptors with retromer, Trends Cell Biol., 15, 68, 10.1016/j.tcb.2004.12.004 · DOI · Paper page
  17. Collins, 2008, The structure and function of the retromer protein complex, Traffic, 9, 1811, 10.1111/j.1600-0854.2008.00777.x · DOI
  18. Rojas, 2008, Regulation of retromer recruitment to endosomes by sequential action of Rab5 and Rab7, J. Cell Biol., 183, 513, 10.1083/jcb.200804048 · DOI · Paper page
  19. Seaman, 2009, Membrane recruitment of the cargo-selective retromer subcomplex is catalysed by the small GTPase Rab7 and inhibited by the Rab-GAP TBC1D5, J. Cell Sci., 122, 2371, 10.1242/jcs.048686 · DOI · Paper page
  20. Nothwehr, 2000, Sorting of yeast membrane proteins into an endosome-to-Golgi pathway involves direct interaction of their cytosolic domains with Vps35p, J. Cell Biol., 151, 297, 10.1083/jcb.151.2.297 · DOI
  21. Shi, 2006, The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain, Nat. Struct. Mol. Biol., 13, 540, 10.1038/nsmb1103 · DOI
  22. Collins, 2008, Structure of Vps26B and mapping of its interaction with the retromer protein complex, Traffic, 9, 366, 10.1111/j.1600-0854.2007.00688.x · DOI
  23. Attar, 2010, The retromer complex, Adv. Enzyme Regul., 50, 216, 10.1016/j.advenzreg.2009.10.002 · DOI
  24. Carlton, 2004, Sorting nexin-1 mediates tubular endosome-to-TGN transport through coincidence sensing of high-curvature membranes and 3-phosphoinositides, Curr. Biol., 14, 1791, 10.1016/j.cub.2004.09.077 · DOI · Paper page
  25. Harbour, 2010, The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics, J. Cell Sci., 123, 3703, 10.1242/jcs.071472 · DOI · Paper page
  26. Viklund, 2009, WAFL, a new protein involved in regulation of early endocytic transport at the intersection of actin and microtubule dynamics, Exp. Cell Res., 15, 1040, 10.1016/j.yexcr.2008.12.004 · DOI
  27. Nakajima, 2006, FKBP133: a novel mouse FK506-binding protein homolog alters growth cone morphology, Biochem. Biophys. Res. Commun., 346, 140, 10.1016/j.bbrc.2006.05.113 · DOI
  28. Gomez, 2009, A FAM21-containing WASH complex regulates retromer-dependent sorting, Dev. Cell, 17, 699, 10.1016/j.devcel.2009.09.009 · DOI · Paper page
  29. Derivery, 2009, The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex, Dev. Cell, 17, 712, 10.1016/j.devcel.2009.09.010 · DOI · Paper page
  30. Carnell, 2011, Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis, J. Cell Biol., 193, 831, 10.1083/jcb.201009119 · DOI · Paper page
  31. Jia, 2010, WASH and WAVE actin regulators of the Wiskott–Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes, Proc. Natl. Acad. Sci. U.S.A., 107, 10442, 10.1073/pnas.0913293107 · DOI · Paper page
  32. Hernandez-Valladares, 2010, Structural characterization of a capping protein interaction motif defines a family of actin filament regulators, Nat. Struct. Mol. Biol., 17, 497, 10.1038/nsmb.1792 · DOI
  33. Tiwari, 1987, Functional equivalents of interferon-mediated signals needed for induction of an mRNA can be generated by double-stranded RNA and growth factors, EMBO J., 6, 3373, 10.1002/j.1460-2075.1987.tb02659.x · DOI
  34. Strochlic, 2007, Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling, J. Cell Biol., 177, 115, 10.1083/jcb.200609161 · DOI · Paper page
  35. Harterink, 2011, A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion, Nat. Cell Biol., 13, 914, 10.1038/ncb2281 · DOI · Paper page
  36. Hierro, 2007, Functional architecture of the retromer cargo-recognition complex, Nature, 449, 1063, 10.1038/nature06216 · DOI · Paper page
  37. Korolchuk, 2007, Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation, J. Cell Sci., 120, 4367, 10.1242/jcs.012336 · DOI · Paper page
  38. Chapman, 1994, Retrieval of TGN proteins from the cell surface requires endosomal acidification, EMBO J., 13, 2305, 10.1002/j.1460-2075.1994.tb06514.x · DOI
  39. Fritz-Laylin, 2010, The genome of Naegleria gruberi illuminates early eukaryotic versatility, Cell, 140, 631, 10.1016/j.cell.2010.01.032 · DOI
  40. Formstecher, 2005, Protein interaction mapping: a Drosophila case study, Genome Res., 15, 376, 10.1101/gr.2659105 · DOI · Paper page
  41. Damer, 2007, Copine A is required for cytokinesis, contractile vacuole function, and development in Dictyostelium, Eukaryot. Cell, 6, 430, 10.1128/EC.00322-06 · DOI
  42. Tomsig, 2003, Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif, J. Biol. Chem., 278, 10048, 10.1074/jbc.M212632200 · DOI
  43. Temkin, 2011, SNX27 mediates retromer tubule entry and endosome-to-plasma membrane trafficking of signalling receptors, Nat. Cell Biol., 13, 715, 10.1038/ncb2252 · DOI · Paper page
  44. Gokool, 2007, Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex, Biochem. J., 408, 287, 10.1042/BJ20070555 · DOI

Cited by

Sorted by citations

Metadata

Authors
Harbour, M. E., Breusegem, S. Y., & Seaman, M. N. J.
Affiliations
Cambridge Institute for Medical Research (CIMR)/Department of Clinical Biochemistry, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 0XY, U.K.
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
2012-01-27
Volume
442
Issue
1
Pages
209-220
ISSN
  • 0264-6021
  • 1470-8728
DOI
10.1042/bj20111761
Cited by
238
References
1
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review