CoChat
Sign Up
Cited by 22T-cell and B-cell ImmunologyLymphoma Diagnosis and TreatmentNF-κB Signaling Pathways

Phospholipase Cγ2 Contributes to Light-Chain Gene Activation and Receptor Editing

Open access
Bai, Li, Chen, Yuhong, He, Yinghong, Dai, Xuezhi, Lin, Xueyan, Wen, Renren, Wang, Demin·2007·Molecular and Cellular Biology
DOI 10.1128/mcb.02273-06PMC PMC1952164
Open this paper in CoChat — free account

Abstract

Phospholipase Cgamma2 (PLCgamma2) is critical for pre-B-cell receptor (pre-BCR) and BCR signaling. Current studies discovered that PLCgamma2-deficient mice had reduced immunoglobulin lambda (Iglambda) light-chain usage throughout B-cell maturation stages, including transitional type 1 (T1), transitional type 2 (T2), and mature follicular B cells. The reduction of Iglambda rearrangement by PLCgamma2 deficiency was not due to specifically increased apoptosis or decreased proliferation of mutant Iglambda+ B cells, as lack of PLCgamma2 exerted a similar effect on apoptosis and proliferation of both Iglambda+ and Igkappa+ B cells. Moreover, PLCgamma2-deficient IgHEL transgenic B cells exhibited an impairment of antigen-induced receptor editing among both the endogenous lambda and kappa loci in vitro and in vivo. Importantly, PLCgamma2 deficiency impaired BCR-induced expression of IRF-4 and IRF-8, the two transcription factors critical for lambda and kappa light-chain rearrangements. Taken together, these data demonstrate that the PLCgamma2 signaling pathway plays a role in activation of light-chain loci and contributes to receptor editing.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/mcb.02273-06

Cites

74 of 74 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • PLCγ2-deficient and inducible PLCγ2−/− mice lack the ability to respond to antigens.

    “Indeed, PLCγ2-deficient and inducible PLCγ2−/− mice have been widely shown to lack the ability to respond to antigens (24, 26, 27, 28).”

    From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [28]

    The Discussion (page unavailable) reports impaired antigen-induced receptor editing in PLCγ2-deficient mice, and the Results (page unavailable) report severely impaired antigen-induced IRF-4 and IRF-8 expression. These are specific impaired responses, not evidence that the mice cannot respond to antigens at all. Our copy also does not establish the claim for inducible PLCγ2−/− mice. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • PLCγ2−/− mice show a block in B cell maturation from transitional 2 to follicular B cells, with increased BCR-induced apoptosis.

    “The first PLCγ2−/− mouse studies showed a block in B cell maturation from transitional 2 to follicular B cells, with these cells also exhibiting increased levels of BCR-induced apoptosis (28, 32).”

    From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [28]

    The Discussion (page not provided) says PLCγ2 is essential for maturation from T2 to follicular B cells, citing previous studies. It does not report increased BCR-induced apoptosis in those cells; the Results (page not provided) instead examine apoptosis in freshly isolated cells, which does not test that claim. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • PLCγ2 is important for light-chain loci activation, required for recombination and receptor editing of autoreactive B cells.

    “The importance of PLCγ2 was additionally shown in terms of light-chain loci activation, required for recombination and receptor editing of autoreactive B cells (28).”

    From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [28]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. [10]Engel, H., A. Rolink, and S. Weiss. 1999. B cells are programmed to activate kappa and lambda for rearrangement at consecutive developmental stages. Eur. J. Immunol. 29:2167-2176. [DOI] [PubMed] [Google Scholar]

Cited by

Sorted by citations

Related

Authors
Bai, L., Chen, Y., He, Y., Dai, X., Lin, X., Wen, R., & Wang, D.
Affiliations
  • Sun Yat-sen University (CN)
  • Versiti Blood Center of Wisconsin (US)
  • Medical College of Wisconsin (US)
Corresponding author
Wang, Demin
ORCID
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
2007-09
Volume
27
Issue
17
Pages
5957-5967
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.02273-06
PMC
PMC1952164
OpenAlex
W2033272706
Cited by
22
References
74
Citations by year
2023: 2 · 2021: 1 · 2019: 1 · 2017: 3 · 2015: 4 · 2014: 2 · 2013: 1
FWCI
0.74
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (R01HL073284)
  • National Institute of Allergy and Infectious Diseases (R01AI052327, R01 AI52327)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Lymphoma Diagnosis and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
Keywords
PLCγ2, lambda light chain, immunoglobulin kappa light chain, receptor editing, B cell receptor signaling, pre-B cell receptor signaling, transitional B cells, follicular B cells, IRF4, IRF8, B cell maturation
MeSH terms
Interferon Regulatory Factor-8, Interferon Regulatory Factor-4, Animals, B-Lymphocytes, Gene Expression Regulation, Immunoglobulin Light Chains, Mice, Inbred C57BL, Mice, Transgenic, Gene Rearrangement, B-Lymphocyte, Signal Transduction, Transcriptional Activation, Apoptosis, Mice, Knockout, Cell Proliferation, Interferon Regulatory Factors, Mice, Phospholipase C gamma, Pre-B Cell Receptors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review